Determination of indinavir in human plasma and its use in pharmacokinetic study

Gonçalves, Talita Mota; Pires, Breno Xavier Fernandes; Bedor, Danilo César Galindo; Souza, Vanessa Cristina de; Abreu, Luis Renato Pires de; Santana, Davi Pereira de

doi:10.1590/s1516-93322007000400018

Cited by 6 publications

(9 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, the proposed method allows monitoring of the therapeutic drug levels in plasma, bioavailability and pharmacokinetic studies (Figures 2 and 3) ( Table 8). The obtained results are with good agreement with the reported plasma concentrations [10,12,31].…”

Section: Application To Patient Samplessupporting

confidence: 90%

“…The results shown in Table 3 are in good agreement with those obtained by the official USP method [13] for the determination of lamivudine, ketoconazole comparison methods [31] for the determination of indinavir. The proposed method is fairly sensitive compared with the official and comparison methods.…”

Section: Application To Dosage Formssupporting

confidence: 82%

“…Official method (13) Proposed method Official method (13) Proposed method Comparison method (31) Table 8: Determination of the studied drugs in µg/mL in patient plasma.…”

Section: Proposed Methodsmentioning

confidence: 99%

“…Several HPLC [31][32][33] and LC-MS [34] methods have been reported for the determination of indinavir. The comparison method [31] uses an analytical column of a Phenomenex Luna® C8 10 mm and a mobile phase was a mixture of di-sodium hydrogen phosphate (0.01 M)+ammonium chloride (0.4%) with pH adjusted to 4.5 (solvent A) and acetonitrile(solvent B), 68:32, v/v, pumped at a flow rate of 1.8 mL/min through the column.…”

Section: Lamivudinementioning

confidence: 99%

See 3 more Smart Citations

Micellar Liquid Chromatographic Determination of Lamivudine, Indinavir and Ketoconazole in Dosage Forms and Biological Fluids

2015

Pharm Anal Acta

View full text Add to dashboard Cite

A simple, reversed phase high performance liquid chromatographic method has been developed for the determination of lamivudine, indinavir and ketokonazole in pharmaceutical preparations, human plasma and urine. The method was conducted using A Shim-pack VP-ODS (150×4.6 mm i.d) stainless steel column at ambient temperature with ultra violet detection at 225 nm. Micellar mobile phase consisted of 0.07 M sodium dodecyl sulphate , 10% n-propanol, 0.3% triethylamine in 0.02 M phosphoric acid (pH 4.5) was used and pumped at a flow rate of 1.2 mL/ min . The calibration curve was rectilinear over the concentration range of (0.05-1.0) µg/mL and (0.2-5.0) and (0.3-5.0) µg/mL lamivudine , indinavir and ketokonazole respectively. The proposed method was successfully applied to the analysis of these drugs in some dosage forms. The method was extended to the in-vitro,

show abstract

Section: Application To Patient Samplessupporting

confidence: 90%

Section: Application To Dosage Formssupporting

confidence: 82%

“…Official method (13) Proposed method Official method (13) Proposed method Comparison method (31) Table 8: Determination of the studied drugs in µg/mL in patient plasma.…”

Section: Proposed Methodsmentioning

confidence: 99%

Section: Lamivudinementioning

confidence: 99%

See 2 more Smart Citations

Micellar Liquid Chromatographic Determination of Lamivudine, Indinavir and Ketoconazole in Dosage Forms and Biological Fluids

2015

Pharm Anal Acta

View full text Add to dashboard Cite

show abstract

“…It may be used in combination with other medications, such as ritonavir, to boost IND activity during highly active anti-retroviral therapy (HAART) [ 2 ]. IND does not affect the early stages of HIV replication; instead, it disrupts the production of infectious HIV, decreasing the contagiousness of the virus [ 3 ]. Therefore, IND works better for controlling HIV infection.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical, spectroscopic, and molecular docking studies of the interaction between the anti-retroviral drug indinavir and dsDNA

Mollarasouli

Dogan‐Topal

Çağlayan

et al. 2020

Journal of Pharmaceutical Analysis

View full text Add to dashboard Cite

In this study, an electrochemical DNA biosensor was developed using a straightforward methodology to investigate the interaction of indinavir with calf thymus double-stranded deoxyribonucleic acid (ct-dsDNA) for the first time. The decrease in the oxidation signals of deoxyguanosine (dGuo) and deoxyadenosine (dAdo), measured by differential pulse voltammetry, upon incubation with different concentrations of indinavir can be attributed to the binding mode of indinavir to ct-dsDNA. The currents of the dGuo and dAdo peaks decreased linearly with the concentration of indinavir in the range of 1.0–10.0 μg/mL. The limit of detection and limit of quantification for indinavir were 0.29 and 0.98 μg/mL, respectively, based on the dGuo signal, and 0.23 and 0.78 μg/mL, respectively, based on the dAdo signal. To gain further insights into the interaction mechanism between indinavir and ct-dsDNA, spectroscopic measurements and molecular docking simulations were performed. The binding constant (K b ) between indinavir and ct-dsDNA was calculated to be 1.64 × 10 8 M −1 , based on spectrofluorometric measurements. The obtained results can offer insights into the inhibitory activity of indinavir, which could help to broaden its applications. That is, indinavir can be used to inhibit other mechanisms and/or hallmarks of viral diseases.

show abstract

Correlating physico-chemical properties of analytes with Hansen solubility parameters of solvents using machine learning algorithm for predicting suitable extraction solvent

Mostafa,

Azim,

ElZaher

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Artificial neural networks (ANNs) are biologically inspired algorithms designed to simulate the way in which the human brain processes information. In sample preparation for bioanalysis, liquid–liquid extraction (LLE) represents an important step with the extraction solvent selection is the key laborious step. In the current work, a robust and reliable ANNs model for LLE solvent prediction was generated which could predict the suitable solvent for analyte extraction. The developed ANNs model takes a set of chosen descriptors for the cited analyte as an input and predicts the corresponding Hansen solubility parameters of the suitable extraction solvent as a model output. Then, from the solvent combination’s appendix, the analyst can identify the proposed extraction solvents' combination for the cited analyte easily and efficiently. For the experimental validation of the model prediction capabilities, twenty structurally diverse drugs belonging to different pharmacological classes were extracted from human plasma. The extraction process was performed using the predicted extraction solvent combination for each drug and quantitively estimated by HPLC/UV methods to assess their extraction recovery. The developed LLE solvent prediction model is in- line with the global trend towards green chemistry since it limits the consumption of organic solvents.

show abstract

Determination of indinavir in human plasma and its use in pharmacokinetic study

Cited by 6 publications

References 28 publications

Micellar Liquid Chromatographic Determination of Lamivudine, Indinavir and Ketoconazole in Dosage Forms and Biological Fluids

Micellar Liquid Chromatographic Determination of Lamivudine, Indinavir and Ketoconazole in Dosage Forms and Biological Fluids

Electrochemical, spectroscopic, and molecular docking studies of the interaction between the anti-retroviral drug indinavir and dsDNA

Correlating physico-chemical properties of analytes with Hansen solubility parameters of solvents using machine learning algorithm for predicting suitable extraction solvent

Contact Info

Product

Resources

About