Determination of indoxyl sulfate by spectrofluorimetric method in human plasma through extraction with deep eutectic solvent
Samira Shafiee,
Siavoush Dastmalchi,
Afshin Gharekhani
et al.
Abstract:A rapid and efficient analytical method was established to quantify indoxyl sulfate (IS) in plasma through extraction technique with a deep eutectic solvent (DES) and spectrofluorimetric method. DES (choline chloride: urea) was mixed with plasma samples for the extraction of IS, followed by the addition of dipotassium hydrogen phosphate (K2HPO4) solution to form an aqueous two-phase system. The fluorescence intensity of IS which was first extracted to the DES-rich-phase and then back-extracted into the salt-ri… Show more
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