Determination of inorganic arsenic in white fish using microwave-assisted alkaline alcoholic sample dissolution and HPLC-ICP-MS

Larsen, Erik Huusfeldt; Engman, Joakim; Sloth, Jens Jørgen; Hansen, Marianne; Jorhem, Lars

doi:10.1007/s00216-004-2815-7

Cited by 50 publications

(23 citation statements)

References 32 publications

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“…Differences in total As content for benthis-feeding and pelagic-schooling 116 species in the North Sea have been previously observed by Larsen et al [14] They found that plaice (mean…”

supporting

confidence: 63%

Arsenic speciation in bodily fluids of harbor seals (Phoca vitulina) and harbor porpoises (Phocoena phocoena)

Kuenstl

Griesel²,

Prange³

et al. 2009

Environ. Chem.

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11 12Environmental context. Marine mammals as top predators ingest a lot of arsenic from their food. In this 13 study liver samples and body fluids of marine mammals from the North and Baltic Seas were investigated 14 for arsenic speciation in order to get some information about their feeding habits. Only organic arsenic 15 compounds with low toxicity were detected in all investigated samples. Although a high quantitative 16 variability was observed, the same compounds were determined in all fluids examined. 18Abstract. The total arsenic concentrations in whole blood of harbor seals (Phoca vitulina) and in liver 19 tissues of harbor porpoises (Phocoena phocoena) were determined using TXRF (total-reflection X-ray 20 fluorescence spectrometry). The total arsenic concentration found in whole blood was lower for fish-fed ). In porpoise liver the arsenic concentrations were higher from carcases found in the North Sea (217- ). 25Furthermore the total arsenic content and arsenic species in urine, plasma, and gastric juice of seals and 26 the urine of porpoises, which were collected from animals at different areas in the North and Baltic Seas, 27were determined using ICPMS (inductively coupled plasma mass spectrometry . The arsenic is almost entirely present in inorganic forms.[1] 49Marine organisms are known to accumulate and convert inorganic arsenic to organic arsenic compounds. 50Mammals such as porpoises and seals living in the marine environment and feeding exclusively on marine 51 organisms are in an exceptional position. As top predators they could accumulate arsenic due to their 52 position in the food web. Goessler et al., reported in 1997 the arsenic concentrations and speciation in a 53 three organism marine food chain starting with a seaweed followed by two gastropods. They showed that 54 arsenic was accumulated and metabolized within this food chain.[2] These results were in contrast to those 55of Kubota et al., who found lower arsenic concentrations in marine mammals (seals and porpoises) 56 compared to those of lower tropic level marine organisms from other studies.[3] Furthermore Kubota et al. 57investigated a possible correlation between arsenic accumulation and gender, age (or body length), or 58 feeding habits in liver tissues of 16 different marine mammal species. The determined arsenic 59concentrations varied widely among species and individuals ranging from 100 to 7680 µg kg -1 dry mass. 60Marine mammals feeding on fish contained lower arsenic concentrations compared to animals feeding on 61cephalopods and crustaceans but no trend with gender or age could be seen.[3] 62Seals feed with regional and seasonal variations on fish, crustaceans, and cephalopods, [4] in contrast to 63 harbor porpoises, which feed mainly on fish and less frequently on molluscs and squids.[5] 64Arsenic contents in tissues of field-collected marine mammals, fish, and invertebrates have been reported 65 in quantities ranges from less than 1000 up to 30000 µg As kg -1 dry mass. [6][7] Total arsenic content in 66 whole blood of...

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“…Differences in total As content for benthis-feeding and pelagic-schooling 116 species in the North Sea have been previously observed by Larsen et al [14] They found that plaice (mean…”

supporting

confidence: 63%

Arsenic speciation in bodily fluids of harbor seals (Phoca vitulina) and harbor porpoises (Phocoena phocoena)

Kuenstl

Griesel²,

Prange³

et al. 2009

Environ. Chem.

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“…Hence, no irreversible protein binding was formed as reported by Larsen et al [26] and Munoz et al [27], despite the high affinity of As III for thiol-groups in proteins [17]. However, since no certified reference materials are available for iAs, the extraction efficiency of iAs from natural incurred samples is difficult to evaluate [28].…”

Section: Extractionmentioning

confidence: 68%

“…However, since no certified reference materials are available for iAs, the extraction efficiency of iAs from natural incurred samples is difficult to evaluate [28]. Several studies have determined the iAs concentration in the CRM TORT-2 (certified for total arsenic), however, no consensus value (concentration range, 0.09-1.1 mg kg −1 ) is reported as recently compiled by Lefroy et al [19,26]. In contrast, the determination of iAs in rice seems not to depend on the extraction approach applied as recently demonstrated in a large interlaboratory study on iAs determination in rice, where consistent results were obtained among different approaches, including the procedure described in the present study [29].…”

Section: Extractionmentioning

confidence: 97%

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Development and validation of an SPE HG-AAS method for determination of inorganic arsenic in samples of marine origin

et al. 2012

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The present paper describes a novel method for the quantitative determination of inorganic arsenic (iAs) in food and feed of marine origin. The samples were subjected to microwave-assisted extraction using diluted hydrochloric acid and hydrogen peroxide, which solubilised the analytes and oxidised arsenite (As(III)) to arsenate (As(V)). Subsequently, a pH buffering of the sample extract at pH 6 enabled selective elution of As(V) from a strong anion exchange solid-phase extraction (SPE) cartridge. Hydride generation atomic absorption spectrometry (HG-AAS) was applied to quantify the concentration of iAs (sum of As(III) and As(V)) as the total arsenic (As) in the SPE eluate. The results of the in-house validation showed that mean recoveries of 101-104% were achieved for samples spiked with iAs at 0.5, 1.0 and 1.5 mg·kg(-1), respectively. The limit of detection was 0.08 mg kg(-1), and the repeatability (RSD(r)) and intra-laboratory reproducibility (RSD(IR)) were less than 8% and 13%, respectively, for samples containing 0.2 to 1.5 mg kg(-1) iAs. The trueness of the SPE HG-AAS method was verified by confirming results obtained by parallel analysis using high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry. It was demonstrated that the two sets of results were not significantly different (P < 0.05). The SPE HG-AAS method was applied to 20 marine food and feed samples, and concentrations of up to 0.14 mg kg(-1) of iAs were detected.

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“…For the determination of inorganic arsenic a method recently applied for the analysis of fish samples was used (Larsen et al. 2005).…”

mentioning

confidence: 99%

Total arsenic and inorganic arsenic content in Norwegian fish feed products

Sloth¹,

Julshamn²,

Lundebye³

2005

Aquac Nutrition

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Samples of complete feedingstuffs for fish, and fishmeals from the Norwegian Fish Feed Monitoring Programme in 2003 were analysed for their total arsenic and inorganic arsenic contents. Microwave assisted wet digestion with nitric acid and hydrogen peroxide was used for the sample preparation for total arsenic analysis by ICPMS. Microwave assisted alkaline‐alcoholic dissolution of the sample was used for liberation of inorganic arsenic prior to analysis by anion‐exchange chromatography High Performance Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry (HPLC–ICPMS). Concentrations in the ranges of 3.4–8.3 and 0.010–0.061 mg kg−1 for complete feedingstuffs were found for total arsenic and inorganic arsenic, respectively. The results are in accordance with typical arsenic levels for fish reported in the literature and indicate that no transformation of chemical arsenic species occurs during the processing of complete feedingstuffs. Several of the complete feedingstuff samples had total arsenic concentrations above the EU maximum content of 6 mg kg−1 in complete feedingstuffs for fish and complete feedingstuffs for fur animals. However, the species of toxicological relevance, i.e. inorganic arsenic, constitutes less than 1.2% of the total arsenic concentration in these samples. The data illustrate that fish feed with high levels of arsenic, but low levels of inorganic arsenic are at risk of being unnecessarily rejected from the fish feed market. Additionally, the results suggest that the maximum level for total arsenic in fish feed should be re‐evaluated and future maximum levels should be based on the chemical species of toxicological relevance, namely inorganic arsenic, rather than on total arsenic.

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Determination of inorganic arsenic in white fish using microwave-assisted alkaline alcoholic sample dissolution and HPLC-ICP-MS

Cited by 50 publications

References 32 publications

Arsenic speciation in bodily fluids of harbor seals (Phoca vitulina) and harbor porpoises (Phocoena phocoena)

Arsenic speciation in bodily fluids of harbor seals (Phoca vitulina) and harbor porpoises (Phocoena phocoena)

Development and validation of an SPE HG-AAS method for determination of inorganic arsenic in samples of marine origin

Total arsenic and inorganic arsenic content in Norwegian fish feed products

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