Determination of insecticide resistance of European grapevine moth [Lobesia botrana (Denis & Schiffermüller) (Lepidoptera: Tortricidae)] populations in vineyards of Manisa province

Abstract

SummaryThis study was carried out to determine the resistance to insecticides of European Grapevine Moth, the main pest of vineyards between 2011-2014. Using the diet method, Lethal Concentration (LC) values of four different insecticides were determined in 10 different European Grapevine Moth populations and then the resistance coefficients were calculated comparing these values with susceptible population. LC50 values were about 6 fold for chlorpyrifos-ethyl in Sarıgöl-2 population, about 5 fold for deltamethrin in Salihli population, about 6 fold for indoxacarb in Ahmetli population, about 6.5 fold for spinosad in Sarıbey population. These results suggest that these populations have developed resistance against the used insecticides. Enzyme activities of AChE, EST and GST were determined in 10 populations. No significant difference was detected in GST enzyme activities of the populations. In Sarıgöl-2 population, AChE enzyme activity was 5 fold while EST enzyme activity was 1.5 fold higher than the susceptible population.

Evaluation of Insecticide Toxicity and Enzymatic Detoxification in Neonate Larvae of European Grapevine Moth, Lobesia botrana Denis & Schiff. (Lepidoptera: Tortricidae)

“…The difference in LC50 values suggests that AL has more susceptibility to chlorpyrifos-ethyl than SAR population. Previously, a similar trend was observed among the field populations, which suggests that L. botrana susceptibility varies in different grapevine production regions (Hatipoglu et al 2015). This might indicate that production areas in SAR or surrounding areas could potentially face a resistant population against chlorpyrifos-ethyl in the near future.…”

“…Several reports are available on insecticide efficacy of L. botrana developmental stages and detoxification enzymatic levels (Irigaray et al 2005;Vassiliou 2011;Civolani et al 2014;Pavan et al 2014;Hatipoglu et al 2015;Navarro-Roldan et al 2017;Pasquini et al 2018;Navarro-Roldan et al 2020). Several reports are available regarding insecticide toxicity on L. botrana (Irigaray et al 2005;Vassiliou 2011;Pavan et al 2014;Hatipoglu et al 2015;Pasquini et al 2018) however, up until now, there is only one insecticide resistance report available for L. botrana (Civolani et al 2014). For that reason, it is important to monitor the insecticide efficacy to provide optimal L. botrana control and mitigate the potential resistance development.…”

“…The toxicity of insecticides has been evaluated both in the field and laboratory on L. botrana stages (egg, pupae, and adult) (Irigaray et al 2005;Ioriatti et al 2011;Civolani et al 2014;Hatipoglu et al 2015). Some of the insecticides were found more effective than others (e.g., emamection benzoate was highly effective compared to indoxacarb) (Civolani et al 2014).…”

“…Furthermore, detoxification enzyme levels can be measured via in vitro methods (Brown & Brogdon 1987;Navarro-Roldan et al 2020) to monitor insecticide resistance levels. Reports about L. botrana enzymatic activity levels are limited (Hatipoglu et al 2015;Navarro-Roldan et al 2020).…”

Comparison of different bioassay methods for determining insecticide resistance in European Grapevine Moth, Lobesia botrana (Denis & Schiffermüller) (Lepidoptera: Tortricidae)

“…In the current study, testing four different insecticides against ten EGM populations from Manisa vineyards using diet incorporation showed that Ahmetli and Alasehir populations were potentially resistant to indoxacarb (Hatipoglu et al, 2015). A high resistance was also detected in Italy using the same bioassay method (Civolani et al, 2014).…”