Determination of interleukin‐5 secretion from drug‐specific activated <i>ex vivo</i> peripheral blood mononuclear cells as a test system for the <i>in vitro</i> detection of drug sensitization

Sachs, B.; Erdmann, Stephan; Baron, Jens Malte; Neis, Mark M.; Masaoudi, Tames Al; Merk, Hans F.

doi:10.1046/j.1365-2222.2002.01382.x

Cited by 67 publications

(72 citation statements)

References 42 publications

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“…9,42,43 Another test used in research concerns the assessment of serum IL-5 levels secreted by peripheral blood mononuclear cells in the presence of the analyzed drug. 44 This test has a diagnostic sensitivity of 92%, which is higher than that of the lymphocyte transformation test (78%) or of the patch tests (55%). This test is not performed as a routine practice.…”

Section: Clinical Picture and Diagnosismentioning

confidence: 93%

Reações alérgicas a medicamentos

Nagão-Dias¹,

Barros-Nunes²,

Coêlho³

et al. 2004

J. Pediatr. (Rio J.)

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Objective: This review addresses the most recent published literature regarding drug allergy, in order to provide physicians with a background for a better understanding of this problem of great relevance for public health. Sources of data:The sources of data for obtaining the original and review articles published in the last 10 years were MEDLINE, Pubmed and Lilacs. The articles chosen for this review relate drug allergy to immunological mechanisms, epidemiology, clinical and laboratory evaluation, skin lesions, clinical management, and re-exposure to the drug.Summary of the findings: Allergic reactions represent one third of adverse drug reactions. They are considered rare but with high morbimortality. Gell & Coombs definition has been useful for classifying some types of drug allergic reactions; however, some still remain without classification because of poor knowledge of the mechanisms involved. The existence of T cell subpopulations with diverse characteristics reveals the complexity of the subject and, at the same time, elucidates several questions raised about it. It was recently postulated a new concept of chemically inert drug presentation to T cells, restricted to the major histocompatibility complex, but in a non-covalent and labile way. In clinical practice, without adequate laboratory tests, it is difficult to correlate clinical symptoms and immunological mechanisms. In vitro and in vivo skin tests have been employed in cases of suspected drug allergy reaction. However, there are very few commercially available reagents.Conclusions: Drug allergy constitutes an important problem in adverse drug reactions because of its potential of morbidity and mortality. It is necessary to emphasize the relevance of pharmacovigilance during treatment of patients, as well as the identification of possible immunological mechanisms involved in the events, through laboratory tests and detailed history and clinical evaluation.

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Section: Clinical Picture and Diagnosismentioning

confidence: 93%

Reações alérgicas a medicamentos

Nagão-Dias¹,

Barros-Nunes²,

Coêlho³

et al. 2004

J. Pediatr. (Rio J.)

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“…For CD69 upregulation, HSA can be used instead of AB serum and cells need to be cultured for only 2-3 days [76]. Alternatively, as used in several studies, cytokines can be measured in the cell culture supernatant: the determination of IL-5 in PBMCs from ten patients with exanthema from amoxicillin showed a sensitivity of 92% and a specificity of 100% [77]. In another study, IFN-c was measured in 36 patients with cutaneous drug reactions [78] and showed a sensitivity of 78%.…”

Section: Topic 2: Drug-reactive T Cellsmentioning

confidence: 99%

T-cell recognition of chemicals, protein allergens and drugs: towards the development of in vitro assays

Martin

Esser

Schmucker

et al. 2010

Cell. Mol. Life Sci.

133

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Chemicals can elicit T-cell-mediated diseases such as allergic contact dermatitis and adverse drug reactions. Therefore, testing of chemicals, drugs and protein allergens for hazard identification and risk assessment is essential in regulatory toxicology. The seventh amendment of the EU Cosmetics Directive now prohibits the testing of cosmetic ingredients in mice, guinea pigs and other animal species to assess their sensitizing potential. In addition, the EU Chemicals Directive REACh requires the retesting of more than 30,000 chemicals for different toxicological endpoints, including sensitization, requiring vast numbers of animals. Therefore, alternative methods are urgently needed to eventually replace animal testing. Here, we summarize the outcome of an expert meeting in Rome on 7 November 2009 on the development of T-cell-based in vitro assays as tools in immunotoxicology to identify hazardous chemicals and drugs. In addition, we provide an This article is dedicated to the memory of our colleague and friend Reinhard Wanner, died 2 April 2010. Cellular and Molecular Life Sciences overview of the development of the field over the last two decades.

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“…B. Typ-1-Sofort-Reaktion vs. Typ-IV-delayed-typehypersensitivity-Reaktion), jetzt auch durch die Aktivierung verschiedener TZell-Subpopulationen (TH2 bzw. TH1) und die Produktion ihrer spezifischen Zytokinmuster pathophysiologisch erklärt werden [4,5]. Die Untersuchung, ob bestimmte Umweltstoffe eher Makrophagen-/Monozyten-Zytokine, TH1-oder TH2-Zytokine induzieren, könnte daher künftig helfen, mögliche Reaktionsmuster gegenüber solchen Substanzen vorherzusagen bzw.…”

Section: Aufklärung Von Pathomechanismenunclassified

“…Eine umfangreiche wissenschaftliche Literatur weist auf die Beteiligung von Zytokinen auch bei Umweltexpositionen und -krankheiten hin.Wegen der aktuellen Bedeutung der Zytokine und der zunehmenden Verfügbar-keit von Untersuchungskits erscheint es zunächst logisch, dass Zytokinbestimmungen im Rahmen der Diagnostik von Umwelterkrankungen vorgenommen und propagiert werden [1, 2,3], zumal sie im Rahmen der Allergiediagnostik bereits einen gewissen Stellenwert haben [4,5,6,7,8]. Jedoch sind die Zytokinmuster sehr variabel und reagieren auf viele Einflüsse, sodass einzelnen Bestimmungen im Blut in der Regel kein diagnostischer Aussagewert zukommt.…”

Section: Introductionunclassified

Bedeutung von Zytokinbestimmungen in der umweltmedizinischen Praxis

2004

Bundesgesundheitsbl - Gesundheitsforsch - Gesundheitsschutz

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Determination of interleukin‐5 secretion from drug‐specific activated ex vivo peripheral blood mononuclear cells as a test system for the in vitro detection of drug sensitization

Abstract: These data suggest a role for the determination of drug-specific IL-5 secretion by ex vivo peripheral blood mononuclear cells for the in vitro detection of drug-sensitization in drug-induced maculopapular exanthems.

Cited by 67 publications

References 42 publications

Reações alérgicas a medicamentos

Reações alérgicas a medicamentos

T-cell recognition of chemicals, protein allergens and drugs: towards the development of in vitro assays

Bedeutung von Zytokinbestimmungen in der umweltmedizinischen Praxis

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