Determination of internal volume and volume distribution of lipid vesicles from dynamic light scattering data

Kojro, Z.; Shi-qing, Lin; Grell, Ernst; Ruf, Horst

doi:10.1016/0005-2736(89)90095-3

Cited by 12 publications

(9 citation statements)

References 27 publications

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“…where Φ is the estimated 'spherical volume' of the vesicles. The value for Φ in the present experimental conditions is negligible [28][29][30][31] and D corr effectively equals D free . From the variables determined above and assuming D vesicles is equal to D bound , rearranging Eq.…”

Section: Resultsmentioning

confidence: 48%

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The association of dimethylsulfoxide and model membranes studied by pulse‐field gradient NMR

Kennedy

Long

Hmel

et al. 2004

Journal of Spectroscopy

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The use of dimethylsulfoxide (DMSO) as a cryoprotectant to reduce cellular injury during freezing is well known, however the intermolecular interactions between this amphiphilic molecule and biological membranes that form the basis of this protection are unknown. DMSO–dipalmitoylphosphatidylcholine (DPPC) vesicle interactions were investigated in pulsed-field gradient NMR (PFGNMR) experiments and spectra analysis allowed for the determination of self-diffusion coefficients for each species present. The mole fraction of DMSO associated with the DPPC vesicles was then calculated from the diffusion coefficients: the mole fraction increased from 14% to 42% as the membrane was heated from below to above the main phase transition temperature.

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Section: Resultsmentioning

confidence: 48%

“…Light scattering, using a 90 • scattering angle, revealed a homogeneous population distribution of vesicles with an average diameter of 100 nm and a narrow size distribution. 31 P NMR experiments demonstrated that the vesicles contained a single bilayer and were therefore unilamellar [26].…”

Section: Resultsmentioning

confidence: 99%

The association of dimethylsulfoxide and model membranes studied by pulse‐field gradient NMR

Kennedy

Long

Hmel

et al. 2004

Journal of Spectroscopy

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“…5 a ). Indeed, the log-normal distribution is a good approximation of the vesicle size distribution ( 9 , 20 , 21 , 22 ). We observe that the increase of the vesicle polydispersity provokes 1) the increase of the average vesicle volume (the curve shift upwards in Fig.…”

Section: Resultsmentioning

confidence: 99%

Physiochemical Modeling of Vesicle Dynamics upon Osmotic Upshift

Gabba

Poolman

2020

Biophysical Journal

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We modeled the relaxation dynamics of (lipid) vesicles upon osmotic upshift, taking into account volume variation, chemical reaction kinetics, and passive transport across the membrane. We focused on the relaxation kinetics upon addition of impermeable osmolytes such as KCl and membrane-permeable solutes such as weak acids. We studied the effect of the most relevant physical parameters on the dynamic behavior of the system, as well as on the relaxation rates. We observe that 1) the dynamic complexity of the relaxation kinetics depends on the number of permeable species; 2) the permeability coefficients (P) and the weak acid strength (pK a-values) determine the dynamic behavior of the system; 3) the vesicle size does not affect the dynamics, but only the relaxation rates of the system; and 4) heterogeneities in the vesicle size provoke stretching of the relaxation curves. The model was successfully benchmarked for determining permeability coefficients by fitting of our experimental relaxation curves and by comparison of the data with literature values (in this issue of Biophysical Journal). To describe the dynamics of yeast cells upon osmotic upshift, we extended the model to account for turgor pressure and nonosmotic volume.

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“…These values are quite comparable to those reported by Kojro et al where liposome internal volumes were calculated from DLS measurements. 23 By knowing the percentage of internalized peptide, relative to the added total amount to the vesicles and the intravesicular volume of liposomes calculated above, the intravesicular concentration of peptide can be determined. An example is Fig.…”

Section: Determination Of the Intravesicular Concentration Of Internamentioning

confidence: 99%

Label-free quantification of cell-penetrating peptide translocation into liposomes

Jobin

Alves

2016

Anal. Methods

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Cell penetrating peptides (CPPs) are small molecules capable of crossing lipid membranes and transporting cargos of varied sizes and nature inside cells. Two cellular uptake mechanisms have been identified: endocytosis and direct translocation through the cellular membrane. Several methods have been proposed to follow and quantify CPP internalization ability both in cells and liposomes, most of them requiring the addition of some sort of label to the CPP. Herein we propose a protocol to test and quantify the translocation ability of CPPs through liposomes by measuring their intrinsic Trp fluorescence and their quenching by acrylamide. Although restrained to CPPs that possess at least one Trp residue in their sequence, the protocol remains applicable to a great number of CPPs. The protocol was applied to three CPPs, including one of the first discovered (penetratin), for their internalization into zwitterionic and anionic liposomes and upon different incubation times. While penetratin and one of its analogs were able to translocate into anionic liposomes, none of the peptides translocated into zwitterionic ones. The newly developed protocol allows for the detection of nanomolar amounts of CPPs inside liposomes. The results obtained are in very good agreement with those determined by other quantification methods.

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Determination of internal volume and volume distribution of lipid vesicles from dynamic light scattering data

Cited by 12 publications

References 27 publications

The association of dimethylsulfoxide and model membranes studied by pulse‐field gradient NMR

The association of dimethylsulfoxide and model membranes studied by pulse‐field gradient NMR

Physiochemical Modeling of Vesicle Dynamics upon Osmotic Upshift

Label-free quantification of cell-penetrating peptide translocation into liposomes

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