Determination of iodine and bromine in plasma and urine by inductively coupled plasma mass spectrometry

Abstract

The simultaneous determination of iodine and bromine in plasma and urine by inductively coupled plasma mass spectrometry, using a Nermag prototype instrument, is described. The sample preparation involves only a 10-fold dilution with a diluent containing europium as an internal standard followed by direct nebulisation in the plasma. The iodine, bromine and europium ions are measured at m/z = 127, 79, and 153, respectively. The sensitivity of the method, with detection limits of 1.6 and 52 micrograms l-1 for iodine and bromine, respectively, is satisfactory for clinical applications. The calibration graphs were linear over the ranges 0-400 micrograms l-1 and 0-40 mg l-1 for iodine and bromine, respectively, which are wide enough for most assays. The recoveries were close to 100% with coefficients of variation of less than 3%. The within-day and between-day reproducibility was about 5%. The concentrations of iodine and bromine in the plasma of 26 healthy individuals were 58 +/- 12 micrograms l-1 and 4.1 +/- 0.9 mg l-1, respectively. The amounts of iodine and bromine eliminated in urine were 94 +/- 97 micrograms per 24 h (range 27-403 micrograms per 24 h) and 3.6 +/- 1.7 mg per 24 h, respectively. These results are in agreement with reported values.

Determination of Total Iodine in Soils by Inductively Coupled Plasma Mass Spectrometry

“…The degree of ionization of iodine is about 29% at absolute temperature, 7,500 K, and electron density, 1 • 1015 cm -3 (Houk 1986). However, iodine at ,ug L -1 level can be analyzed by ICP-MS because of its high sensitivity and selectivity (Allain et al 1990;Baumann 1990;Akabane and Shimizu 1991;Schramel and Hasse 1994).…”

Use of iodized salt in the households improves the iodine status of pregnant women and school-age children in Donetsk, Ukraine: A double-blind randomized controlled trial

“…Для измерения концентрации йода в моче была использована масс спектрометрия с индуктивно связанной плазмой [1] в лаборатории "Биомедици на" (Донецк).…”

Validation and Comparison of Two Calibration Methods for the Measurement of Stable Iodine in the Urinary Matrix by ICP-MS: Standard Addition vs. External Calibration

“…Among them, Allain et al determined a LOD (calculated as twice the standard deviation of the background signal) in urine of 1.6 µg·L −1 with a 10-fold dilution in acid media using external calibration method [19]. Other authors have carried out measurements on twenty urine samples with a 50-fold dilution factor in alkaline media using external calibration and matrix-matched calibration solutions [28].…”

“…Iodine measurement by ICP-MS in various matrices (such as foodstuffs or biological fluids) has been already addressed in previous studies [19] [20] [21] [22]. For all these reasons, this analytical technique was naturally chosen for the measurement of iodine in the urinary matrix which was carried out by standard additions (referred as calibration 1) and external calibration methods (thereafter abbreviated as calibration 2).…”