Determination of iohexol and iothalamate in serum and urine by capillary electrophoresis

Houcke, Sofie K. Van; Seaux, Liesbeth; Cavalier, Étienne; Speeckaert, Marijn M.; Dumoulin, Els; Lecocq, Elke; Delanghe, Joris

doi:10.1002/elps.201600084

Cited by 7 publications

(3 citation statements)

References 17 publications

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“…The most encountered analytical techniques are liquid chromatography with ultraviolet detection (LC-UV) [6,7] or coupled to tandem mass spectrometry (LC-MS/MS) [8]. Other techniques such as capillary electrophoresis and X-ray fluorescence are also reported in the literature [9,10].…”

Section: Introductionmentioning

confidence: 99%

Determination of iohexol by capillary blood microsampling and UHPLC-MS/MS

Ion

Legoff

Cavalier

et al. 2019

Journal of Pharmaceutical Analysis

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One of the most important tools used to evaluate kidney function in the context of chronic kidney disease or other renal function related pathologies is the exploration of glomerular filtration rate (GFR). Iohexol is up to this moment a good candidate molecule for the GFR assessment since it exhibits minimum protein binding rates and minimum extra-renal clearance, being neither secreted nor reabsorbed at the tubular level. This study proposes and evaluates a new LC-MS/MS method for the iohexol determination from capillary blood, prelevated using volumetric absorbative microsampling (VAMS) systems. As an alternative to VAMS, a brand new HemaPEN ® device for micro-prelevation was also tested. A new high throughput sample preparation protocol adapted for iohexol quantification from whole blood VAMS samples was developed. The medium term stability study of iohexol in dried whole blood VAMS samples that was conducted showed a good stability of this molecule for up to 12 days. By collecting only 10 μL of blood, iohexol can be analyzed from dried whole blood VAMS samples for concentration ranges between 1 and 250 μg/mL. Due to the analyte stability in VAMS for up to 12 days, this approach might be successfully applied for GFR assessment for clinical cases allowing minimum invasiveness and even delayed analysis.

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Section: Introductionmentioning

confidence: 99%

Determination of iohexol by capillary blood microsampling and UHPLC-MS/MS

Ion

Legoff

Cavalier

et al. 2019

Journal of Pharmaceutical Analysis

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“…Клиренс йогексола может осуществляться либо клиренсом из плазмы, либо клиренсом с мочой [46,47]. Анализ йогексола можно проводить с помощью высокоэффективной жидкостной хроматографии (ВЭЖХ), химического измерения, основанного на определении содержания йода, рентгеновской адсорбционной спектроскопии или капиллярного электрофореза [48]. В клинической практике ВЭЖХ является наиболее используемым методом из-за ее чувствительности, специфичности, а также гибкости.…”

Section: экзогенные маркерыunclassified

Modern markers for determining the functional ability of the kidneys in urological practice.

Kazachenko¹,

Voytko²,

Prosyannikov³

et al. 2023

ECU

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Introduction. Over the past decade, there has been a progressive increase in the number of patients with chronic kidney disease (CKD). Early diagnosis of renal insufficiency allows to prevent irreversible consequences leading to disability and significant medical and economic costs. Purpose: to analyze modern markers for determining the functional ability of the kidneys. Materials and methods. A search was carried out in PubMed and Library, according to the keywords creatinine, urea, glomerular filtration rate (GFR), clearance, formulas for calculating creatinine clearance, cystatin C, β2-microglobulin, β-bound protein, NGAL, KIM-1, chronic kidney disease, chronic renal failure, urolithiasis. We found 389 sources no older than 10 years (published after 2012) that were relevant to the topic of the review. 80 articles were selected directly for citation in the review. Results. GFR assessment and detection of albuminuria are the most effective methods, but biomarkers used to calculate GFR are subject to a number of factors and do not allow assessing kidney function in real time. For early prediction of acute renal injury, the most suitable biomarkers are considered to be: NGAL and KIM-1 and IL-18. Equations combining cystatin C and creatinine r work better than equations using only cystatin C or creatinine, especially in situations where it is necessary to confirm chronic kidney disease. The combination of creatinine, cystatin C and the ratio of urine albumin to creatinine improves the stratification of the risk of progression of kidney disease and mortality. The latest developments in biomarkers allow us to hope that in the near future the assessment of the functional ability of the kidneys will be carried out in real time and will become a general clinical practice of a modern doctor. Conclusion. Modern biomarkers of the functional ability of the kidneys allow not only to evaluate the function, but also to screen for diseases, conduct differential diagnosis, and evaluate the effectiveness of the treatment. The modern approach to the diagnosis of renal dysfunction should be based not only on its diagnosis, but also on determining the topic of nephron damage.

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“…Based on personal communications with vendors, HPLC-UV analysers cost $65,000-200,000, while an LC-MS/MS analyser costs $320,000-500,000. Capillary electrophoresis has been used for iohexol measurement but has not been extensively validated [25]. HPLC-UV likely offers the best compromise between technical expertise required, robustness, cost and availability in the African context.…”

Section: Filtration Markersmentioning

confidence: 99%

Measurement of glomerular filtration rate and its current status in African countries GFR measurement and its status in Africa

Holness¹,

Westhuizen²,

Davids³

et al. 2022

AJN

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Glomerular filtration rate (GFR) is usually estimated rather than measured as this only requires measurement of an endogenous filtration marker. In certain clinical settings a more accurate measure of GFR is essential. The most commonly used endogenous filtration marker is creatinine. Exogenous filtration markers include nonradiopharmaceuticals such as inulin, iohexol and unlabelled iothalamate, or radiopharmaceuticals such as 51Crethylenediaminetetraacetic acid (51Cr-EDTA) and 99mTc-diethylenetriaminepentaacetic acid (99mTc-DTPA). Inulin is considered an ideal filtration marker but the clearance of iothalamate, 99mTc-DTPA, 51Cr-EDTA and iohexol have all been shown to have sufficient accuracy for measuring GFR. For radiopharmaceuticals, a well counter is required to measure the amount of activity in patient samples. Iohexol or unlabelled iothalamate require samples to be measured using high performance liquid chromatography with ultraviolet detection (HPLC-UV), liquid chromatography-tandem mass spectrometry (LC-MS/MS) or x-ray fluorescence (XRF). Due to the practical challenges of measuring urinary clearance, measurement of GFR in clinical settings is almost exclusively based on plasma clearance of a filtration marker. This can follow a long-established approach based on the ratio of the tracer administered to the area under the plasma concentration curve. Alternatively, a single plasma sample giving an apparent volume of distribution at a given time point can be used to accurately measure GFR. While techniques exist for the measurement of GFR in a number of African countries, preliminary evidence suggests that facilities are very limited. There is a need for support for both equipment and training to establish GFR measurement facilities in several centres on the continent. Keywords: measured glomerular filtration rate;, mGFR;, Africa

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Determination of iohexol and iothalamate in serum and urine by capillary electrophoresis

Cited by 7 publications

References 17 publications

Determination of iohexol by capillary blood microsampling and UHPLC-MS/MS

Determination of iohexol by capillary blood microsampling and UHPLC-MS/MS

Modern markers for determining the functional ability of the kidneys in urological practice.

Measurement of glomerular filtration rate and its current status in African countries GFR measurement and its status in Africa

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