Determination of irinotecan and its metabolite SN-38 in dried blood spots using high-performance liquid-chromatography with fluorescence detection

Hahn, Roberta Zilles; Arnhold, Priscila Costa; Andriguetti, Natália Bordin; Schneider, Anelise; Klück, Helena Moreira; Reis, Simone L. dos; Bastiani, Marcos Frank; Kael, Igor; Silva, Anne Caroline Cezimbra da; Schwartsmann, Gilberto; Antunes, Marina Venzon; Linden, Rafael

doi:10.1016/j.jpba.2017.11.079

Cited by 23 publications

(22 citation statements)

References 19 publications

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“…Not all of the published methods involving DBS have reported the assessment of the influence of hematocrit on the assay performance. However, many reports published recently have incorporated the testing of the influence of hematocrit on quantitative data gathered on the analytes in question (Antunes, Raymundo, de Oliveira, et al, ; Antunes, Raymundo, Wagner, et al, ; Boons et al, ; Hahn et al, ; Hawwa et al, ; Irie et al, ; Jager, Rosing, Schellens, & Beijnen, ; Kralj et al, ; Nijenhuis et al, ; Renata et al, ; Saini, Sulochana, Kiran, et al, ; Saini, Sulochana, Zainuddin, & Mullangi, ; Singhal, Shah, Shah, Sharma, & Shrivastav, ; Xie et al, ; Xu et al, ). The DBS assay may be significantly impacted by hematocrit values since hematocrit is a sole determinant that has an effect on the viscosity of the blood; the viscosity of blood plays a role in the flux and diffusive properties of the analytes that are present in blood through DBS sampling system.…”

Section: Key Considerations For Dbs Methods Developmentmentioning

confidence: 99%

“…Hahn et al () used blood with a hematocrit value of 40% to test the effect of spot volume of 30, 40 and 55 μL for the simultaneous determination of irinotecan and its active metabolite, SN‐38, in patient samples. The reason for using a 30–55 μL spotting volume was to mimic the likely drop size from finger prick sampling in the patients.…”

Section: Key Considerations For Dbs Methods Developmentmentioning

confidence: 99%

“…The calibration cure was prepared using a blood spot volume of 50 μL. The analytical data suggested that the accuracy of the determination of either irinotecan or SN‐38 was not influenced by the spot blood volume at two levels of QC samples (Hahn et al, ).…”

Section: Key Considerations For Dbs Methods Developmentmentioning

confidence: 99%

“…Generally sensitivity of a fluorescence detector is 10–1000 times higher than that of a UV detector. Two methods (Hahn et al, ; Renata et al, ) have been reported for the quantification of etoposide and irinotecan and its metabolite using HPLC with fluorescence detection in clinical samples. Renata et al () adopted a simple liquid extraction using a combination of MeOH–ACN–H 2 O and separation was performed using a reverse‐phase column connected to a fluorescence detector.…”

Section: Literature Data Compilation and Summarymentioning

confidence: 99%

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Review of DBS methods as a quantitative tool for anticancer drugs

Sulochana

Daram

Srinivas

et al. 2018

Biomedical Chromatography

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An overview of published dried blood spot (DBS) methods for the quantitation of various classes of anticancer drugs from clinical and preclinical studies is presented. The increased reporting of DBS methods in the literature for quantitation of various classes of drugs is a testimony to their utility in bioanalytical applications. While DBS offers several advantages as compared with conventional wet sampling techniques, there remain a number of nuances that may impede the assay adaptability of DBS method in routine quantitative bioanalysis. This review covers several case studies of DBS application in the quantitation of anticancer drugs. Some perspectives are provided on the optimization of the DBS method with respect to the selection of DBS card, spot volume, hematocrit effect and other regular validation parameters, which are essential in quantitative bioanalysis. Some thoughts are provided on the existing gaps in the DBS method and possible remedial measure(s) to address such gaps.Although DBS methods have great potential, there is the need for a global consensus including regulatory support on the type of validation experiments to be performed to support quantitative data.

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Section: Key Considerations For Dbs Methods Developmentmentioning

confidence: 99%

Section: Key Considerations For Dbs Methods Developmentmentioning

confidence: 99%

Section: Key Considerations For Dbs Methods Developmentmentioning

confidence: 99%

Section: Literature Data Compilation and Summarymentioning

confidence: 99%

See 2 more Smart Citations

Review of DBS methods as a quantitative tool for anticancer drugs

Sulochana

Daram

Srinivas

et al. 2018

Biomedical Chromatography

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“…Therapeutic Drug Monitoring (TDM) represents an important tool to establish the best dose regimen, reduce side effects and personalize the chemotherapeutic treatment. 4 Several methods have been developed to quantify irinotecan and SN-38 in human plasma by HPLC-UV, LC-MS and direct MALDI-MS quantification, 5,6,7,8 however these techniques require specialised personnel, are time consuming and expensive. A point of care device may overcome these drawbacks and allow the patients to monitor directly and personalize the therapy.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of Irinotecan in Human Plasma: Sensitive Fluorescent Nanogels by Molecular Imprinting

Berti¹,

Resmini²,

Toffoli³

et al. 2019

Preprint

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A series of fluorescent molecularly imprinted nanogels to detect irinotecan (CPT11) were prepared and characterized. A set of amino acids and napthalimide polymerisable derivatives allowed to obtain polymers as soluble fluorescent nanoparticles by high dilution imprinted synthesis. The direct detection of irinotecan in human plasma was obtained by fluorescence quenching of the naphtalimide-based imprinted materials. The plasma sample treated with acetonitrile allowed the detection of irinotecan in the 10nM – 30μM range. The LOD was 9.4 nM, with within-run variability 10% and day to day variability 13%.<br>

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Mechanism and optimization of supramolecular complexation‐enhanced fluorescence spectroscopy for the determination of SN‐38 in plasma and cells

et al. 2020

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Quantitative detection of two different forms of SN‐38 in biological samples is, currently, cumbersome and difficult. A revisit to the mechanism of supramolecular complexation‐enhanced fluorescence spectroscopy helps to optimize the determination of SN‐38 in plasma and the cellular pharmacokinetics in A549 cells based on the supramolecular complexation. Firstly, the inclusion mechanism dominated by thermodynamic constants was determined by measuring kinetic/thermodynamic parameters (kon, koff, ΔG, ΔH, ΔS). On this basis, the best effect of fluorescence sensitization was optimized through screening the interaction conditions (cyclodextrin species and concentrations, drug levels, temperature, pH of the buffer, and reaction time). Furthermore, the proportional relationship between the concentration of the inclusion complex and the fluorescence intensity was confirmed. Finally, a highly sensitive, selective spectrofluorimetric method was established and validated for quantitative analysis of the lactone and carboxylate molecular states of SN‐38 plasma levels in rats and cell membrane transfer kinetics in A549 cell lines. The limits of detection for the lactone and carboxylate forms in plasma were found to be 0.44 ng·ml−1 and 0.28 ng·ml−1, respectively. Precision and accuracy met the requirements of biological samples analysis. The proposed detection method provided a reference for elucidating the biodistribution of SN‐38.

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Determination of irinotecan and its metabolite SN-38 in dried blood spots using high-performance liquid-chromatography with fluorescence detection

Cited by 23 publications

References 19 publications

Review of DBS methods as a quantitative tool for anticancer drugs

Review of DBS methods as a quantitative tool for anticancer drugs

Monitoring of Irinotecan in Human Plasma: Sensitive Fluorescent Nanogels by Molecular Imprinting

Mechanism and optimization of supramolecular complexation‐enhanced fluorescence spectroscopy for the determination of SN‐38 in plasma and cells

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