Determination of kaempferol in extracts from Lilium candidum L. Liliaceae by means of liquid chromatography

Abstract

There is an optimized, validated and implemented method for determination of kaempferol through liquid chromatography in terms of isocratic separation in the proportion: 0.05% phosphoric acid and acetonitril (50:50) v/v. The limits of detection (LOD) and quantification (LOQ) were determined. The content of kaempferol in samples from Lilium candidum L. with the use of different concentrations of extract agent and after releasing kaempferol from dermal semisolid medicaments was investigated. The samples were prepared by various producers. The results obtained proved that the implemented method shows good validated parameters and is favourable for determination of kaempferol in samples from Lilium candidum L. It was kaempferol that evaluated best from the samples that were prepared by extraction of 80% ethanol. The contents in the samples were determined using the method, after releasing kaempferol from dermal semisolid medicaments.

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“…The separations were performed using XBridge TM C18 column (4.60 × 250 mm; particle diameter, 5 µm; Thermo Scientific, U.S). The mobile phase for kaempferol consisted of 0.05 % orthophosphoric acid and acetonitrile at a ratio of 1:1 (v/v) [13], while for quercetin its mobile phase consisted of methanol: acetonitrile: deionized water at a ratio of 3 : 1 : 1 v/v/v. The flow rate of the mobile phase was set at 0.3 mL/min with 20 μL of injection volume [14].…”