Determination of kallikrein by radioimmunoassay in human body fluids

Abstract

A sensitive and specific radioimmunoassay for human urinary kallikrein was developed, which allows tissue kallikrein determination in human urine, saliva, pancreatic juice, bile and sweat. In several body fluids a kallikrein-like antigen was found, but not in gastric juice and breast milk. According to gel filtration studies, complex formation of kallikrein with serum proteins or different molecular weight forms of kallikrein in serum and urine may be assumed. Pancreatic kallikrein secretion follows the same pattern after stimulation with secretin and cholecystokinin as trypsin and chymotrypsin in normal individuals. In chronic pancreatitis the kinetic behaviour remains unchanged with respect to the enzyme secretion, but the secretion of kallikrein is reduced to about 20%.

Kinin B2 Receptor-Coupled Signal Transduction in Human Cultured Keratinocytes

“…The kinin-forming enzyme, tissue kallikrein, has been localized in the secretory fundus of sweat glands (Poblete et al, 1991), and several authors have described its biochemical properties (Mann et al, 1980;Mayfield et al, 1989;Hibino et al, 1994). Recently, the mRNA for true kallikrein (KLK1, tissue kallikrein, kininogenase) and those for the B1R and B2R have been reported for the normal human skin, and in biopsies of patients with some skin diseases (Schremmer-Danninger et al, 1999).…”

Activation of kinin B1receptor triggers differentiation of cultured human keratinocytes

“…Several components of the kinin system have been found to occur in the human skin. The kinin‐forming enzyme, tissue kallikrein hk1 (kininogenase) has been localized to the secretory fundus of sweat glands 1 and its major biochemical properties have been described 2–4 . Under normal conditions, the immunoreactive kinin‐forming substrate (kininogen) locates in the interstitial tissue space between keratinocytes, 5 making feasible the concept that kinins are formed in the skin.…”

Proceedings of the Physiological Society, 30‐31 March 1990,University College London Meeting: Communications

“…(Fraki et al 1970). More recently, immunoreactive tissue kallikrein has been measured in human sweat (Mann et al 1980). This finding has been confirmed by Mayfield et al (1989), who suggested that eccrine sweat glands responding to heat or pilocarpine stimulation secrete readily measurable levels of tissue kallikrein.…”