Determination of kinetic parameters of 1,3-propanediol fermentation by Clostridium diolis using statistically optimized medium

Abstract

1,3-propanediol (1,3-PD) is a chemical compound of immense importance primarily used as a raw material for fiber and textile industry. It can be produced by the fermentation of glycerol available abundantly as a by-product from the biodiesel plant. The present study was aimed at determination of key kinetic parameters of 1,3-PD fermentation by Clostridium diolis. Initial experiments on microbial growth inhibition were followed by optimization of nutrient medium recipe by statistical means. Batch kinetic data from studies in bioreactor using optimum concentration of variables obtained from statistical medium design was used for estimation of kinetic parameters of 1,3-PD production. Direct use of raw glycerol from biodiesel plant without any pre-treatment for 1,3-PD production using this strain investigated for the first time in this work gave results comparable to commercial glycerol. The parameter values obtained in this study would be used to develop a mathematical model for 1,3-PD to be used as a guide for designing various reactor operating strategies for further improving 1,3-PD production. An outline of protocol for model development has been discussed in the present work.

Strategies for Large-scale Production of Polyhydroxyalkanoates

“…In addition, it does not account for interactions between the various factors, and is therefore unable to depict their combined effect on the responses under investigation. 29,82 When dealing with complex, multi-variable systems such as biological processes, such an approach is not useful, as it is unable to screen the significant process factors and optimize their values. This limitation can be overcome by the Design-of-Experiment (DoE) methodology or statistical experimental planning, which involves generation of well-defined output factors or responses, such as biomass/product concentration or productivity, from a set of defined input factors.…”

Continuous bioproduction of 1,3‐propanediol from biodiesel raw glycerol: Operation with free and immobilized cells of Clostridium butyricum DSM 10702

“…The parameters were quite similar to those reported for various Clostridium butyricum strains cultivated on crude glycerol. [8,[24][25][26] The maximum theoretical biomass yields (Y ' X / S ) reported in the literature range from 0.03 to 0.057 g/g whereas the coefficient Y 1,3-PD / S ranges from 0.52 to 0.57 and the yield Y 1,3-PD/X takes values from 9.4 to 18.05 g/g.…”

Influence of fluid dynamic conditions on 1,3‐propanediol production from glycerol by Shimwellia blattae: carbon flux and cell response

“…Biomass growth rate is described as usual by the logistic equation . The production of 1,3‐propanediol is described according to a hyperbolic expression, while mixed acid route by‐products were lumped into a single compound, as usually done in the kinetic modeling of complex reacting systems and also described by a hyperbolic model …”