Determination of kynurenic acid in human serum and its correlation with the concentration of certain amino acids

Fukushima, Takeshi; Mitsuhashi, Shogo; Tomiya, Masayuki; Iyo, Masaomi; Hashimoto, Kenji; Toyo’oka, Toshimasa

doi:10.1016/j.cca.2006.09.019

Cited by 33 publications

(25 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In a previous study [14], we used this approach to identify the alkaloid kynurenic acid (KYNA), an endogenous glutamate antagonist with preferential action at the glycine site of the N-methyl d-aspartate-receptor [15], as the main marker of botanical origin of chestnut honey; we confirmed its presence, although in smaller quantities in other honeys from arboreal species, probably because of contamination with chestnut honey (eucalyptus, lime tree, almond).…”

Section: Introductionsupporting

confidence: 75%

A solid-phase extraction procedure coupled to 1H NMR, with chemometric analysis, to seek reliable markers of the botanical origin of honey

Beretta

Caneva

Regazzoni

et al. 2008

Analytica Chimica Acta

View full text Add to dashboard Cite

Section: Introductionsupporting

confidence: 75%

A solid-phase extraction procedure coupled to 1H NMR, with chemometric analysis, to seek reliable markers of the botanical origin of honey

Beretta

Caneva

Regazzoni

et al. 2008

Analytica Chimica Acta

View full text Add to dashboard Cite

“…Column-switching high-performance liquid chromatography (HPLC) [32][33][34] was performed for the fluorescence determination of KYNA levels in the microdialysis samples by using a previously reported technique [31]. The reported KYNA recovery through the probe, with this technique, is 20.4 ± 2.76% [31].…”

Section: Kyna Level Determinationmentioning

confidence: 99%

Changes in Extracellular Kynurenic Acid Concentrations in Rat Prefrontal Cortex After d-Kynurenine Infusion: An In vivo Microdialysis Study

et al. 2009

Self Cite

View full text Add to dashboard Cite

Using a microdialysis technique, we continuously infused D-kynurenine (KYN) (0, 50, and 100 microM) into the prefrontal cortices (PFCs) of male Sprague-Dawley rats. We then used column-switching high-performance liquid chromatography to assess the alterations in the concentration of kynurenic acid (KYNA)-an antagonist of N-methyl-D-aspartate and alpha7 nicotinic acetylcholine receptors-in the extracellular fluid in the PFC. Local infusion of D-KYN into the PFC remarkably increased the extracellular KYNA concentration, indicating that D-KYN is metabolized to KYNA in the PFC. The D-KYN-induced increase in KYNA levels was significantly attenuated by the co-administration of 3-methylpyrazole-5-carboxylic acid (AS057278)-a specific inhibitor of D-amino acid oxidase (DAAO). These results suggest that DAAO may be involved in the production of KYNA from D-KYN in the PFC in vivo.

show abstract

“…10,20 In a plastic vial (0.25 ml), 10 ll of rat plasma was added with 10 ll of H 2 O and 80 ll of 50 mM ammonium acetate in MeOH, and the mixture was vigorously mixed for 2 min by a vortex mixer. After centrifugation at 600g for 5 min, the supernatant was diluted twice with the mobile phase of the HPLC, namely H 2 O/CH 3 CN (95/5) containing 0.1% AcOH.…”

Section: Determination Of Plasma Kynamentioning

confidence: 99%

“…An aliquot (20 ll) of the resultant solution after filtration with Millex 1 (Nihon Millipore K.K., Tokyo, Japan) was injected into the HPLC system. 20,21 KYNA was quantified by a calibration curve constructed by the peak area plotted against the KYNA concentration.…”

Section: Determination Of Plasma Kynamentioning

confidence: 99%

See 1 more Smart Citation

Alteration of kynurenic acid concentration in rat plasma following optically pure kynurenine administration: A comparative study between enantiomers

et al. 2008

Self Cite

View full text Add to dashboard Cite

L-Kynurenine (KYN), a tryptophan metabolite, is metabolized to kynurenic acid (KYNA), which is an antagonist of N-methyl-D-aspartate and alpha7 nicotinic acetylcholine receptors, by kynurenine aminotransferase (KAT) I and KAT II. In this study, optically pure KYN, namely L-KYN or D-KYN, was administered intraperitoneally to male Sprague-Dawley rats (16.3 micromol kg(-1)), and the change in plasma KYNA was investigated by using column-switching high-performance liquid chromatography (HPLC) with fluorescence detection. Unexpectedly, no remarkable alteration in the plasma KYNA was observed when a natural isomer, L-KYN, was administered, whereas plasma KYNA concentration was unequivocally increased when an unnatural isomer, D-KYN, was administered. Serum protein bindings of L-KYN and D-KYN were also studied, and the protein binding of L-KYN (approximately 65%) in rat serum was larger than that of D-KYN (approximately 12%), suggesting that D-KYN may be easily incorporated and metabolized in tissues during blood circulation to generate KYNA in mammals. In addition, the increase in plasma KYNA by the administration of D-KYN was suppressed in rats pretreated with a selective inhibitor of D-amino acid oxidase (DAAO), 5-methylpyrazole-3-carboxylic acid (80 mg/kg). These results suggest that DAAO might be responsible for the production of KYNA from D-KYN in vivo.

show abstract

Determination of kynurenic acid in human serum and its correlation with the concentration of certain amino acids

Cited by 33 publications

References 29 publications

A solid-phase extraction procedure coupled to 1H NMR, with chemometric analysis, to seek reliable markers of the botanical origin of honey

A solid-phase extraction procedure coupled to 1H NMR, with chemometric analysis, to seek reliable markers of the botanical origin of honey

Changes in Extracellular Kynurenic Acid Concentrations in Rat Prefrontal Cortex After d-Kynurenine Infusion: An In vivo Microdialysis Study

Alteration of kynurenic acid concentration in rat plasma following optically pure kynurenine administration: A comparative study between enantiomers

Contact Info

Product

Resources

About