Determination of L‐trantinterol in rat plasma by using chiral liquid chromatography‐tandem mass spectrometry

Abstract

A simple, sensitive, and rapid method for determination of L-trantinterol in rat plasma was developed for the first time by using LC coupled to MS/MS based on chiral stationary phase. A baseline separation of the enantiomers of trantinterol was achieved on a Chirobiotic V column, using a mixture of acetonitrile-methanol-ammonia-acetic acid (80:20:0.01:0.02, v/v/v/v) as the mobile phase. The detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring mode via ESI. The calibration curve was linear in concentration range from 0.270 to 108 ng/mL in plasma with the lower limit of quantification of 0.270 ng/mL. The intra- and interday precision (relative standard deviation) values were within 10.9% and the accuracy (relative error) was from 2.6 to 9.2% at all quality control levels. The method has been successfully applied to a study of L-trantinterol pharmacokinetics in rats.

Bidirectional Chiral Inversion of Trantinterol Enantiomers After Separate Doses to Rats

“…However, the chiral inversion of phenylethanolamine derivatives has not been documented. Our previous study found that a chiral inversion of trantinterol occurred to some extent in rats . The purpose of this article is to report our observation of substantial bidirectional chiral inversion after oral and intravenous administration of (−)‐ and (+)‐trantinterol to rats.…”

“…A previously validated stereospecific assay was modified. An aliquot of IS solution (100 μL) was pipetted into a 10‐mL glass tube and evaporated to dryness.…”

An LC–MS/MS method for simultaneous determination of trantinterol and its major metabolite in rat plasma and its application to a comparative pharmacokinetic study

“…However, currently, only a handful of methods have been reported for the quantification of trantinterol in biological samples based on LC-MS/MS [9][10][11][12][13]. Furthermore, all the previous in vivo pharmacokinetics studies of trantinterol after oral administration had http://dx.doi.org/10.1016/j.jchromb.2015.07.022 1570-0232/© 2015 Elsevier B.V. All rights reserved.…”

Simultaneous quantification of trantinterol and its metabolites in human urine by ultra performance liquid chromatography–tandem mass spectrometry

“…Few methods have been reported for the quantification of trantinterol in biological fluids [3][4][5]. And only one published literature reported a LC-MS/MS method [6] for the simultaneous determination of trantinterol and its major metabolites in rat urine and feces.…”