Determination of Lactoferrin in Camel Milk by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry Using an Isotope-Labeled Winged Peptide as Internal Standard

Abstract

An ultrahigh-performance liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of lactoferrin in camel milk based on the signature peptide. The camel lactoferrin was purified by heparin affinity chromatography and then used to screen tryptic signature peptides. The signature peptide was selected on the basis of sequence database search and identified from the tryptic hydrolysates of purified camel lactoferrin by ultrahigh-performance liquid chromatography and quadrupole time-of-flight tandem mass spectrometry. The pretreatment procedures included the addition of isotope-labeled winged peptide and the disposal of lipids and caseins followed by an enzymatic digestion with trypsin. Analytes were separated on an Acquity UPLC BEH 300 C18 column and then detected on a triple-quadrupole mass spectrometer in 7 min. The limits of detection and quantification were 3.8 mg kg−1 and 11 mg kg−1, respectively. The recoveries ranged from 74.5% to 103.6%, with relative standard deviations below 7.7%. The validated method was applied to determine the lactoferrin in ten samples collected from Xinjiang Province.

Anti-Oxidative and Immuno-Protective Effect of Camel Milk on Radiation-Induced Intestinal Injury in C57BL/6 J Mice

“…CM has higher lactoferrin content than milk from cows, goats, and buffaloes. 34 A study revealed that lactoferrin could increase the survival rate of mice exposed to X-ray irradiation by decreasing the levels of serum IL-6 and TNF-a and reducing the expression of radiation-induced IKKa/b and NF-kB. 19 In conclusion, our findings demonstrated that CM reduces the level of inflammatory cytokines by regulating the HMGB/ TLR4/NF-kB/MyD88 pathway and the antioxidant capacity to decrease radiation-induced intestinal damage (Figure 7).…”

“…CM has higher lactoferrin content than milk from cows, goats, and buffaloes. 34 A study revealed that lactoferrin could increase the survival rate of mice exposed to X-ray irradiation by decreasing the levels of serum IL-6 and TNF-α and reducing the expression of radiation-induced IKKα/β and NF-κB. 19 …”

An Efficient Heparin Affinity Column Purification Method Coupled with Ultraperformance Liquid Chromatography for the Quantification of Native Lactoferrin in Breast Milk

“…e sample extract was added after treatment with the buffer. After the sample solution was eluted completely, the column was rinsed with 10 [22]. e eluent was collected and centrifuged at 9000 rpm for 5 min before UPLC analysis.…”

Isolation, Purification and Characterization of a-Lactalbumin from Camel Milk and Study its Antioxidant Activity in Oils

“…Camel milk whey proteins, caseins, and LAB have been the subject of a number of studies looking at their potential antioxidant, immune-regulating, anti-inflammatory, and anti-microbial effects. Antioxidant properties were found in camel milk in all studies [4,5]. The oxidation reaction in foods is one of the most difficult challenges facing the food and medical sciences because it is directly related to food safety and consumer health.…”