Determination of Lead, Cadmium and Mercury in Microwave- digested Foodstuffs by RP-HPLC with an On-line Enrichment Technique

Abstract

A new method for the simultaneous determination of lead, cadmium and mercury ions in microwave-digested foodstuffs by reversed-phase high-performance liquid chromatography combined with on-line enrichment technique has been developed. The foodstuff samples were digested by microwave digestion. The lead, cadmium and mercury ions can be precolumn derivatized with 5,10,15,20-tetrakis(3-aminophenyl)porphine (T3APP) to form color chelates; then, the Hg-T3APP, Cd-T3APP and Pb-T3APP chelates can be enriched and separated on a valve switching HPLC system combined with on-line enrichment technique. The linearity ranges are 0.01 -120 µg/l for each metal ion. The detection limits (S/N = 3) of lead, cadmium and mercury are 1.2 ng/l, 0.5 ng/l and 0.8 ng/l, respectively. This method was applied to the determination of lead, cadmium and mercury in foodstuffs with good results.

Determination of cobalt, nickel and iron at trace level in natural water samples by in-column chelation-reversed phase high-performance liquid chromatography

“…Up to now, various chelating agents, such as thiosemicarbazones , pyridylazo derivatives (Oszwaldowski and Okada 1999), oxines (Takeuchi et al 2001), resorcinol derivatives (Takahashi et al 2006), porphyrin (Huang et al 2003), and dithiocarbamates (Dilli and Tong 1999) have been used as derivatization agents for multimetal analysis by RP-HPLC. Among these agents, only few studies are available on the use of 4-(2-pyridylazo) resorcinol (PAR) to form 4-(2-pyridylazo)resorcinolato complex with pre-column derivatization for the determination of multimetal ions using RP-HPLC (Takahashi et al 2006;Peng et al 2005).…”

Colorimetric Assay for Determination of Lead (II) Based on Its Incorporation into Gold Nanoparticles during Their Synthesis

“…Several methods for Pb 2+ analysis have been developed in the past decade, including ones based on atomic absorption spectrometry (AAS), atomic emission spectrometry (AES), inductively coupled plasma mass spectrometry (ICP-MS), anodic stripping voltammetry, and reversed-phase high-performance liquid chromatography coupled with UV-vis or fluorescence detection [12–17]. With regard to sensitivity and accuracy, these methods are all efficient tools for Pb 2+ detection, but they are time-consuming, expensive, and/or require sophisticated equipment.…”

Study on the Solid Phase Extraction of Hg(II)-SBDTR Chelate with C18Disks and Its Application to the Determination of Mercury in Tobacco and Tobacco Additive

“…For this reasons, a wide variety of spectrophotometric methods for the determination of mercury have been reported. [9][10][11][12][13][14][15][16][17][18][19][20][21] Each chromogenic system has its advantages and disadvantages with respect to sensitivity, selectivity and rapidity.…”