Determination of leaf pigments by HPLC after extraction with N,N-dimethylformamide: Ecophysiological applications

Bergweiler, Paul

doi:10.1016/0098-8472(86)90031-6

Cited by 20 publications

(8 citation statements)

References 3 publications

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“…Samples were removed between 0900 and 1100 hours under full sun conditions, on the last day of the field work. Because liquid nitrogen was not available and electricity was only sporadically available, leaf samples were immediately placed into 1 ml of dimethylformamide (DMF) in glass vials (Bergweiler and Lütz 1986) and transported to the laboratory on ice within a picnic cooler. The transit time was 2 days and ice was periodically replaced.…”

Section: Xanthophyll Pigmentsmentioning

confidence: 99%

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Photosynthetic responses of tree seedlings in grass and under shrubs in early-successional tropical old fields, Costa Rica

Loik

Holl

2001

Oecologia

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Only recently have studies addressed the effect of early-colonizing vegetation on tree seedling survival and growth during secondary succession in tropical old fields, and few studies have elucidated the physiological responses of tree seedlings to different vegetational communities. We compared growth and various photosynthetic parameters for seedlings of four rain-forest tree species, Cedrela tonduzii, Inga punctata, Ocotea whitei, and Tapirira mexicana, growing in areas of pasture grass and shrubs in early-successional abandoned pasture in Costa Rica; in addition, we made measurements for two species in forest gaps. We tested the general hypothesis that early-colonizing shrubs facilitate growth of forest tree seedlings. Specifically, we measured microclimate, growth, CO assimilation, stomatal conductance, photosystem II quantum yield (ΦPSII), and xanthophyll pigment pools for all seedlings. Photosynthetic flux density (PFD) was higher under grass than shrubs or forest gaps, but was highly variable in each growth environment. For three of the four species, height growth was greatest in the grass compared to the shrubs and forest gaps; growth was similar below grass and shrubs for O. whitei. Photosynthetic capacity, apparent quantum yield, and stomatal conductance did not vary across habitats, but light compensation point and PFD at light saturation tended to be higher in the grass compared to forest and shrub growth environments. Water use efficiency differed across growth environments for three of the species. For plants in ambient PFD and dark-adapted plants, the efficiency of excitation energy transfer through PSII was lowest for plants in the grass compared to shrubs and forest gaps and also differed across species. Measurement of steady-state responses of ΦPSII to increasing PFD indicated a significant effect of growth environment at low PFD for all species and significant effects at high PFD only for I. punctata. All species exhibited a high degree of midday xanthophyll de-epoxidation in the different growth environments. Xanthophyll pigment pool size on an area basis was highest in the grass compared to shrubs and forest gaps for all four species. The results suggest that shrubs do not provide a facilitative effect for growth or photosynthesis for ~1.5-year-old seedlings of these four species. We conclude that site differences in success of tree seedlings during succession are a result of complex interactions of facilitation and competition and are not simply based on physiological responses to PFD.

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Section: Xanthophyll Pigmentsmentioning

confidence: 99%

“…Pigments were separated and quantified using high-performance liquid chromatography, using modified methods of Bergweiler and Lütz (1986) and Thayer and Björkman (1990). Pigment extracts in DMF were mixed with 2 volumes of 50% methanol before injection of 100 µl into a Perkin-Elmer Series 4 liquid chromatograph.…”

Section: Xanthophyll Pigmentsmentioning

confidence: 99%

Photosynthetic responses of tree seedlings in grass and under shrubs in early-successional tropical old fields, Costa Rica

Loik

Holl

2001

Oecologia

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“…Pigment contents were determined spectrophotometrically following extraction with dimethylformamide containing 0.1% (v/v) -tocopherol (Bergkeiler and Lu¨tz, 1986). Photosynthetic capacity (A ) was determined by infrared gas analysis with a minicuvette system (Walz, Effeltrich, Germany) under light and CO saturation, whereas net photosynthesis was determined under ambient exposure chamber conditions (Lange et al, 1986).…”

Section: Photosynthetic Parameters and Assessment Of Visible Symptomsmentioning

confidence: 99%

Memory Effects in the Action of Ozone on Conifers

Langebartels

Heller

Führer³

et al. 1998

Ecotoxicology and Environmental Safety

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“…N,N-dimethylformamide has been used successfully for pigment extraction in previous studies, [1,[13][14][15][16][17][18] suggesting it may be an acceptable substitute for acetone. N,N-dimethylformamide has been used successfully for pigment extraction in previous studies, [1,[13][14][15][16][17][18] suggesting it may be an acceptable substitute for acetone.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Acetone and N,N-Dimethylformamide for Pigment Extraction in Turfgrass

Stiegler¹,

Bell

Maness

2004

Comm Soil Sci Plant An

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The extraction and isolation of plant pigments are critical parts of the procedures used for pigment analyses by plant scientists. Various organic solvents have been used to liberate chlorophylls and carotenoids, including xanthophylls, from plant tissue. Acetone extraction is the most common extractant, but other solvents, such as N,N-dimethylformamide (DMF), have been used successfully. The objectives of this study were to compare the repeatability of acetone and DMF extraction for turfgrass pigment analysis and to investigate which extraction method is more appropriate for sample preparation before analysis using high-performance liquid chromatography (HPLC). Leaf tissue was collected from three plots ORDER REPRINTS of a mixed sward of creeping bentgrass (Agrostis stolonifera L.) and annual bluegrass (Poa annua L.) that were differentially treated in the field with magnesium (Mg), iron (Fe), and/or a plant growth regulator to encourage a range of leaf pigment concentrations. Variation among tissue samples, pigment extraction, and the HPLC process was used to compare the precision of acetone with DMF extraction. Results indicated that both acetone and DMF extraction procedures exhibited acceptable variation, but DMF extraction was more consistent at each procedural level. Procedural levels included variation among tissue samples (coefficient of variation ¼ 11.2 < 12.1), variation among HPLC measurements (coefficient of variation ¼ 2.1 < 2.9), and variation among extracted samples (coefficient of variation ¼ 4.8 < 12.7). The DMF extraction also yielded 84% more neoxanthin, 53% more violaxanthin, 47% more lutein, 104% more chlorophyll b, 145% more chlorophyll a, and 14% more b-carotene than acetone. Overall, pigment extraction with DMF produced more quantitative and more reliable results than acetone extraction.

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Determination of leaf pigments by HPLC after extraction with N,N-dimethylformamide: Ecophysiological applications

Cited by 20 publications

References 3 publications

Photosynthetic responses of tree seedlings in grass and under shrubs in early-successional tropical old fields, Costa Rica

Photosynthetic responses of tree seedlings in grass and under shrubs in early-successional tropical old fields, Costa Rica

Memory Effects in the Action of Ozone on Conifers

Comparison of Acetone and N,N-Dimethylformamide for Pigment Extraction in Turfgrass

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