Determination of Levofloxacin, Norfloxacin, and Moxifloxacin in Pharmaceutical Dosage Form or Individually Using Derivative UV Spectrophotometry

Abstract

The first, second, third, and fourth-order derivative spectrophotometric methods by using the peak—zero (P - O) and peak-peak (P - P) techniques of measurement have been developed for the determination of levofloxacin, norfloxacin, and moxifloxacin in their combined pharmaceutical dosage form or individually for levofloxacin, norfloxacin, and moxifloxacin. Linearity was achieved at 2–20 µg/ml. The procedure is Simple, quick, and precise. The developed method is sensitive and precise. simple, accurate, and cost-effective, and exhibited a good correlation coefficient (R2 = 0.9998) and excellent mean recovery (99.20–100.08%), indicating a high degree of precision of the methods. This approach was successfully used to determine the levofloxacin, norfloxacin, and moxifloxacin content in marketed pharmaceutical dosages. The method's linearity, and accuracy, were confirmed statistically and through recovery tests. The results showed that the method can be used for routine analysis in bulk and commercial formulations.

Determination of levofloxacin, norfloxacin, and moxifloxacin in pharmaceutical dosage form or individually using derivative UV spectrophotometry

“…Derived UV-spectrophotometry is a method that provides both qualitative and quantitative information from spectra in disputable bands. It involves using the first or higher derivatives of absorbance concerning wavelength for analysis purposes [1]. Derivative spectroscopy was first introduced in the 1950s and demonstrated various advantages; however, it received limited attention due to the challenges of generating derivative spectra with early UV-Visible spectrophotometers.…”