Determination of lignans inSchisandra chinensis using micellar electrokinetic capillary chromatography

Abstract

Micellar electrokinetic capillary chromatography (MEKC) has been developed as a promising method for the determination of lignans in plant samples. The separation conditions have been optimized with respect to the different parameters including sodium dodecyl sulfate (SDS) and acetonitrile concentration, pH of the background electrolyte, separation voltage, and capillary temperature. The background electrolyte consisting of 40 mM SDS and 35% acetonitrile in 10 mM tetraborate buffer (pH 9.3) was found to be the most suitable electrolyte for this analysis. The applied voltage of 28 kV (positive polarity) and the capillary temperature 25 degrees C gave the best separation of lignans. The interday reproducibility of the peak areas and the migration times was below 2.0%. The results of MEKC analyses were compared with those obtained by capillary electrochromatography (CEC) and reversed-phase high-performance liquid chromatography (RP-HPLC). The possibilities of using this method for the determination of lignans in drug and in serum samples were also tested.

Simultaneous Separation of Eight Lignans in Forsythia suspensa by β-Cyclodextrin-Modified Capillary Zone Electrophoresis

“…No matter which solvent described in the literature was used as a background electrolyte [10,11], it did not improve the poor separation of compounds 1 – 8 . This fact could be explained by the low-acid property and at least one hydroxy group in each core skeleton of compounds 1 – 8 .…”

“…This fact could be explained by the low-acid property and at least one hydroxy group in each core skeleton of compounds 1 – 8 . In contrast, only methoxy and/or methylenedioxy groups occur in lignans tested in MEKC [10,11]. Thus, CZE was further attempted in this study.…”

“…The micellar electrokinetic chromatography (MEKC) methods have been proposed for the analysis of diarylbutyrolactone-type, tetrahydrofuran-type, diarylbutane-type, aryltetralin-type and dibenzocyclooctene-type lignans in Phyllanthus urinaria , P. niruri and Schisandra chinensis [10,11]. Furthermore, non-aqueous and pressurized CE methods have been reported for analysis of dibenzocyclooctene-type lignans in S. chinensis for quality evaluation [12,13].…”

Dibenzo[a,c]cyclooctadiene lignans of the genus Schisandra: importance, isolation and determination

“…Sterbova et al [97] published a micellar electrokinetic capillary chromatography (MEKC) as a promising method for the determination of lignans in plant samples. The separation conditions were optimized with respect to different parameters, including sodium dodecyl sulfate (SDS) and acetonitrile concentration, pH of the background electrolyte, separation voltage and capillary temperature.…”

Separation procedures applicable to lignan analysis

“…Stěrbová et al reported MEKC method with SDS as a micellar phase for the determination of lignans in Schisandra chinensis [113]. Because of the low solubility of lignans up to 35% acetonitrile had to be added to the background electrolyte consisting of 40 mM SDS in 10 mM tetraborate buffer (pH 9.3).…”