Determination of lipid class concentrations in seawater by thin-layer chromatography with flame ionization detection

Delmas, Roger; Parrish, Christopher C.; Ackman, R. G.

doi:10.1021/ac00272a018

Cited by 67 publications

(22 citation statements)

References 47 publications

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“…TLC/FID was performed with Chromarods S11 and an Iatroscan MK I11 (Iatron Laboratories, Tokyo) using modifications of previously described procedures (Delmas et al 1984, Parrish & Ackman 1985, as detaded in Parrish (1986).…”

Section: Methodsmentioning

confidence: 99%

“…However, it is the monoglycerides that might be expected to accumulate in the environment because lipases tend to act more efficiently on diglycerides than on monoglycerides (Gurr & James 1980). The high levels of dissolved AMPL found in surface waters (Delmas et al 1984, Parrish 1986) could indicate that this does occur. The Rf value of monoglycerides is identical to that of AMPL in the solvent systems used for the Chromarod TLC separations (Parrish 1986).…”

Section: Lipids and Inorganic Nitrate During 1982 And 1984 Bloomsmentioning

confidence: 99%

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Time series of particulate and dissolved lipid classes during spring phytoplankton blooms in Bedford Basin, a marine inlet

Parrish¹

1987

Mar. Ecol. Prog. Ser.

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The Chromarod-Iatroscan TLC/FID system was used to measure h p~d classes in the dissolved and particulate fractions of seawater samples taken dunng spnng blooms In Bedford Basln, a small inlet in eastern Canada. Polar hpids were the major dissolved lipids present during the bloon~s. Dissolved l i p~d t~m e series indicate decreased phytoplankton exudation and increased bacterial uptake of lipid classes at the t~m e of the chlorophyll maximum. There is evidence to suggest algal uptake of dissolved phospholip~ds. T h~s polar l i p~d class may be used by autotrophs as a source of N and P when inorganic nutrient levels are depleted. The energy-rich storage component, triglyceride, became the major particulate lipid class from the time of the chlorophyll maximum onwards. Other particulate hpid classes present after the chlorophyll maxlmunl suggest that in addition to increased energy storage, there was a greater degree of degradahon of hplds and larger numbers of microheterotrophs.

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Section: Methodsmentioning

confidence: 99%

Section: Lipids and Inorganic Nitrate During 1982 And 1984 Bloomsmentioning

confidence: 99%

Time series of particulate and dissolved lipid classes during spring phytoplankton blooms in Bedford Basin, a marine inlet

Parrish¹

1987

Mar. Ecol. Prog. Ser.

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“…The Canadian maritime biogeochemical province, represented in multiple cases by lM of the lipids or more, actually spans heterogeneous coastal, slope, and open water regimes. The cited investigations average data collected in shore or else over the continental shelf (Delmas et al 1984;Parrish et al 1992). Hence they probably reflect enhanced primary production.…”

Section: Data Comparisonsmentioning

confidence: 99%

Global distribution and surface activity of macromolecules in offline simulations of marine organic chemistry

et al. 2015

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Organic macromolecules constitute a high percentage of remote sea spray. They enter the atmosphere through adsorption onto bubbles followed by bursting at the ocean surface, and go on to influence the chemistry of the fine mode aerosol. We present a global estimate of mixed-layer macromolecular distributions, driven by offline marine systems model output. The approach permits estimation of oceanic concentrations and bubble film surface coverages for several classes of organic compound. Mixed layer levels are computed from the output of a global ocean ecodynamics model by relating the macromolecules to standard biogeochemical tracers. Steady state is assumed for labile forms, and for longer-lived components we rely on ratios to existing transported variables. Adsorption is then represented through conventional Langmuir isotherms, with equilibria deduced from laboratory analogs. Open water concentrations locally exceed one micromolar carbon for the total of proteins, polysaccharides and refractory heteropolycondensates. The shorter-lived lipids remain confined to regions of strong biological activity. Results are evaluated against available measurements for all compound types, and agreement is generally well within an order of magnitude. Global distributions are further estimated for both fractional coverage of bubble films at the air-water interface and the two-dimensional concentration excess. Overall, we show that macromolecular mapping provides a novel tool for the comprehension of oceanic surfactant patterns. These results may prove useful in planning

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“…This allowed the residual hydrocarbon to separate from other lipid classes. Polar lipids separation was improved by using a last development in dichloromethane : methanol: water (5 : 4 : 1) according to Delmas et al (1984). It separated phospholipids from non-lipid material which remained at the origin.…”

Section: Methodsmentioning

confidence: 99%

Cellular and extracellular carbohydrates and lipids from marine bacteria during growth on soluble substrates and hydrocarbons

Goutx¹,

Acquaviva²,

Bertrand³

1990

Mar. Ecol. Prog. Ser.

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The variability of extracellular excretions of 4 marine bacterial strains was investigated during growth on soluble (acetate or lactate) and insoluble (hydrocarbons) substrates. Cellular and extracellular protein, carbohydrate and lipid contents were estimated at the end of exponential growth phase. Emulsifying capacity of the cell-free culture medium was estimated. Lipid classes were characterized by using thin layer chromatography coupled with flame ionization detection. All strains were found to excrete organic compounds in their culture medium whatever the substrate. Carbohydrate and lipid compounds were the main products whereas proteins were excreted in lesser amounts. Nevertheless, the adaptation of the marine strains to insoluble substrate was characterized by enhanced lipid excretions (mainly phospholipids) which correlated well with higher emulsifying activity on this substrate. These excretions represented 8.6 to 156.2 and 4.3 to 101.6 yg per mg bacterial carbon, for carbohydrates and lipids respectively, according to a recent estimate of the bacterial protein to carbon ratio. These data show that inputs of dissolved organic compounds through bacterial activity could have important consequences on the physico-chemical characteristics of seawater in area where bacterial biomass is concentrated.

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Determination of lipid class concentrations in seawater by thin-layer chromatography with flame ionization detection

Cited by 67 publications

References 47 publications

Time series of particulate and dissolved lipid classes during spring phytoplankton blooms in Bedford Basin, a marine inlet

Time series of particulate and dissolved lipid classes during spring phytoplankton blooms in Bedford Basin, a marine inlet

Global distribution and surface activity of macromolecules in offline simulations of marine organic chemistry

Cellular and extracellular carbohydrates and lipids from marine bacteria during growth on soluble substrates and hydrocarbons

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