Determination of Lipoprotein Z-Specific IgA in Tuberculosis and Latent Tuberculosis Infection

Xiao, Jianwei; Xiong, Yanqing; Chen, Yingying; Xiao, Yang-jiong; Ji, Ping; Yong, Li; Wang, Shujun; Zhao, Guoping; Cheng, Qin; Lu, Shuaiyao; Wang, Ying

doi:10.3389/fcimb.2017.00495

Cited by 12 publications

(18 citation statements)

References 44 publications

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“…Another previous study found that anaerobic bacteria such as Fusobacterium, treponema, Tannerella and Prevotella rapidly increased in mature plaque over 4 days, and they were associated with gingivitis and periodontitis [21]. Moreover, periodontopathic plaque shows higher richness compared to healthy plaque, and this increases with the FIGURE 3 Relative abundance of the phylum-level community (A) and the genus-level community (B) identified in non-RF and RF plaque samples; genera marked with an asterisk (*) exhibited significant differences in relative abundance (Wilcoxon rank-sum test, P < .05) FIGURE 4 Linear discriminant analysis (LDA) effect size (LEfSe) analysis of bacterial taxa present in RF plaque (red bar) and non-RF plaque (gray bar) at the genus level periopathogenicity [16,22,23]. It is therefore reasonable that the red fluorescence emitted by dental plaque indicates increased maturity and pathogenicity.…”

Section: Discussionmentioning

confidence: 99%

Detection of dental plaque and its potential pathogenicity using quantitative light‐induced fluorescence

Lee

Jong

Kim

2019

Journal of Biophotonics

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Quantitative light‐induced fluorescence (QLF) technology can detect some dental plaque as red fluorescence. This in vivo study aimed to identify the microbial characteristics of red fluorescent (RF) dental plaque using 16S rRNA gene sequencing and evaluate the correlations between RF plaque and the clinical symptoms of dental diseases. Paired supragingival plaque samples collected from each 10 subjects and consisted of RF and non‐RF dental plaques as observed by QLF technology using a 405 nm blue light source for excitation. The characteristics of the bacterial communities in the RF and non‐RF plaque samples were compared by sequencing analysis. An increase in microbial diversity was observed in RF plaque compared with the non‐RF plaque. There were significant differences in the community compositions between the 2 types of dental plaque. Periodontopathic bacteria were significantly more abundant in the RF plaque than non‐RF plaque. The fluorescence intensity of RF plaque was significantly related to the proportion of the periodontopathic bacterial community and the presence of gingival inflammation. In conclusion, the plaque red fluorescence is associated with changes in the microbial composition and enrichment of periodontopathic pathogens, which suggests that RF plaque detected by QLF technology could be used as a risk indicator for gingival inflammation.

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Section: Discussionmentioning

confidence: 99%

Detection of dental plaque and its potential pathogenicity using quantitative light‐induced fluorescence

Lee

Jong

Kim

2019

Journal of Biophotonics

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“…Our previous work has also reported that LppZ-specific IgA levels in TB patients and latent TB infection (LTBI) individuals were dramatically higher than that in healthy controls (HCs). LppZ-specific IgA level decreased substantially along with the anti-TB treatment (Xiao et al, 2017). What’s more, its potential as a new TB vaccine candidate antigen was evaluated with enhanced immune protection against M.tb challenge in mouse models (under revision manuscript).…”

Section: Introductionmentioning

confidence: 99%

Identification of HLA-A2-Restricted Mycobacterial Lipoprotein Z Peptides Recognized by T CellsFrom Patients With ActiveTuberculosis Infection

Liu

Wang

et al. 2018

Front. Microbiol.

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Identification of HLA-restricted peptides derived from mycobacterial antigens that are endowed with high affinity and strong antigenicity is not only of interest in tuberculosis (TB) diagnostics and treatment efficacy evaluation, but might also provide potential candidates for the development of therapeutic vaccines against drug-resistant TB. Our previous work demonstrated that lipoprotein Z (LppZ) displayed high immunogenicity and antigenicity in active TB patients. In the present study, ten HLA-A2-restricted LppZ peptides (LppZp1-10) were predicted by bioinformatics, among which LppZp7 and LppZp10 were verified to possess high affinity to HLA-A2 molecules using T2 cell-based affinity binding assay. Moreover, results from ELISpot assay showed that both LppZp7 and LppZp10 peptides were able to induce more IFN-γ producing cells upon ex vivo stimulation of PBMC from HLA-A2+ active TB (ATB) patients as compared to those from healthy controls (HCs). Also, the numbers of LppZp7 and LppZp10-specific IFN-γ producing cells exhibited positive correlations with those of ESAT-6 peptide (E6p) or CFP-10 peptide (C10p) in ATB. Interestingly, stimulation with LppZp7/p10 mixture was able to induce higher intracellular expression of IFN-γ and IL-2 cytokines in CD8+ and CD4+ T cells from ATB as compared to HC, associated with lower expression of TNF-α in both CD8+ and CD4+ T cells. Taken together, HLA-A2-restricted LppZp7 and LppZp10 peptides display high immunoreactivity in HLA-matched ATB patients demonstrated by high responsiveness in both CD8+ and CD4+ T cells. With the ability to induce strong antigen-specific cellular responses, LppZp7 and LppZp10 are of potential value for the future applications in the prevention and control of TB.

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“…However, our study was not designed to speci cally address the effect of alcohol in humoral response of TB patients, more studies are needed to elucidate these serological ndings. Moreover, lower positive IgA PPE 59 frequency in IT patients may be related to treatment, as recently described for LppZ IgA low response after 2 months of anti-TB treatment [38], however, our study was not designed to follow up the treated patients. No other demographic or clinical characteristic was associated with Ab positivity among Italian/immigrants, especially for IgG, which is mainly due to the low number of individuals who were positive for the test.…”

Section: Discussionmentioning

confidence: 87%

PPE 59 immunoreactivity in tuberculosis patients: IgA immunodominance to that IgG and IgM

Mulinari¹,

Sardella²,

Silva³

et al. 2020

Preprint

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Background: The role of protein families PE/PPE remains relatively enlightening. The gene rv3429, coding the PPE59 protein, is one of the 12 members of the PPE subfamily, and it is absent in all BCG strains. Although elicits low T cell response, there are no clues for the ability to induce a humoral response. Methods: We cloned, expressed, and analyzed by ELISA IgG, IgM and IgA anti PPE59 in 212 sera. Of them, 69 were from tuberculosis patients’ residents in Italy (TBIT, 12 native and 67 immigrants), the remaining 133 are Brazilians citizens (BR). Pulmonary (pTBIT) or extrapulmonary (eTBIT) clinical forms were 54 or 25. The pTBBR were 52 and 81 non-TB patients, including 10 non-tuberculous mycobacteria infected (NTM). Results: Keeping the specificity at 97%, IgA sensitivity decreased from pTBBR (53%) to pTBIT (38%) and eTBIT (28%), with an overall sensitivity of 42.7% and moderate accuracy (61.8 %), while IgG showed significant lower (p<0.0001) performance, 21%, 3%, 0%, 9% and 37%, respectively, at specificity of 83%. Groups were not discriminate by IgM. False-positive NTM IgG was high. Combination 16kDa IgG, previously performed only on Bazilians’ sera, plus PPE 59 IgA results increased sensitivity to 71% at 87.4% specificity. The overall smear microscopy (SM) diagnosis was 70.8% and a combination of SM/IgA increased sensitivity to 74% (p=0.01), mainly among SM- cases. Among pTBBR, all these rapid tests, including SM, sensitivity reach 86.5% (p=0.001). Positive IgA polarization was significant in extensive lung disease (p=0.001) and alcohol users (p=0.04). Conclusion: Although PPE59 IgA independently has moderate accuracy on TB diagnosis, together with other biomarkers contributes to improving its detection. Moreover, the polarized reactivity deserves further investigation.

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Determination of Lipoprotein Z-Specific IgA in Tuberculosis and Latent Tuberculosis Infection

Cited by 12 publications

References 44 publications

Detection of dental plaque and its potential pathogenicity using quantitative light‐induced fluorescence

Detection of dental plaque and its potential pathogenicity using quantitative light‐induced fluorescence

Identification of HLA-A2-Restricted Mycobacterial Lipoprotein Z Peptides Recognized by T CellsFrom Patients With ActiveTuberculosis Infection

PPE 59 immunoreactivity in tuberculosis patients: IgA immunodominance to that IgG and IgM

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