2007
DOI: 10.1590/s0100-879x2007000100009
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Determination of low tetanus or diphtheria antitoxin titers in sera by a toxin neutralization assay and a modified toxin-binding inhibition test

Abstract: A method for the screening of tetanus and diphtheria antibodies in serum using anatoxin (inactivated toxin) instead of toxin was developed as an alternative to the in vivo toxin neutralization assay based on the toxin-binding inhibition test (TOBI test). In this study, the serum titers (values between 1.0 and 19.5 IU) measured by a modified TOBI test (Modi-TOBI test) and toxin neutralization assays were correlated (P < 0.0001). Titers of tetanus or diphtheria antibodies were evaluated in serum samples from gui… Show more

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Cited by 15 publications
(9 citation statements)
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“…Additionally, were also measured the potencies of the D and T components of DTP and DTPlow, by means of Toxin Binding assays. 20 , 21 Both preparations induced high and comparable levels of neutralizing antibodies against tetanus toxin (DTP 23.8 IU/ml and DTPlow 46.4 IU/ml; p = 0.27) and diphtheria toxin (DTP 66 IU/ml and DTPlow 32 IU/ml; p = 0.12), demonstrating that the LOS extraction had no effect on the immunogenicity against T and D antigens induced by the combined vaccine (data not shown).…”
Section: Resultsmentioning
confidence: 95%
“…Additionally, were also measured the potencies of the D and T components of DTP and DTPlow, by means of Toxin Binding assays. 20 , 21 Both preparations induced high and comparable levels of neutralizing antibodies against tetanus toxin (DTP 23.8 IU/ml and DTPlow 46.4 IU/ml; p = 0.27) and diphtheria toxin (DTP 66 IU/ml and DTPlow 32 IU/ml; p = 0.12), demonstrating that the LOS extraction had no effect on the immunogenicity against T and D antigens induced by the combined vaccine (data not shown).…”
Section: Resultsmentioning
confidence: 95%
“…In vitro tests for measuring tetanus and diphtheria antitoxin levels in serum were carried out by a standardized modified toxin-binding inhibition (TOBI) test as described by Hendriksen et al (13) but employing diphtheria toxoid instead of toxin (19,31,32).…”
Section: Methodsmentioning
confidence: 99%
“…Serosurveys could be used to monitor progress toward the achievement and maintenance of MNTE given challenges of reliably assessing TT coverage through vaccination history (14). For in vitro assays of tetanus seroprotection, gold standards are the DAE (double-antigen enzyme-linked immunosorbent assay [ELISA]) and toxin binding inhibition (ToBI) tests, which are not commercially available (15)(16)(17)(18)(19). Indirect tetanus ELISAs exist, but they overestimate antibody levels in the low seroprotective range (0.01 to 0.2 IU/ml) (15,(19)(20)(21).…”
mentioning
confidence: 99%