Determination of Lysine, Arginine, and Histidine by High Temperature Paper Chromatography

Ornithine, putrescine and cadaverine in farm silage

“…The following method was adopted finally, the procedure being the same for both eluates. 13 Whatman 3MM, 23 cm paper was prepared by dipping in phosphate buffer of pH 6.8 (200 ml of 0.067~-disodium phosphate and 250 ml of 0 ~067~-potassium dihydrogen phosphate) and allowing to dry at room temperature. A measured sample of eluate was applied to the starting line as a short band 2.5 cm wide with an 'Agla' micrometer syringe.…”

An Enzyme‐Free Amperometric Sensor Based on Self‐Assembling Ferrocene‐Conjugated Oligopeptide for Specific Determination of L‐Arginine

“…Conventional techniques for the assay of L‐ Arg included high performance liquid chromatography (HPLC), [ 8 ] mass spectrometry (MS), [ 9 ] ion exchange paper chromatography, [ 10 ] thin‐layer chromatography, [ 11 ] high temperature paper chromatography, [ 12 ] etc . However, these methods generally involve rigorous sample treatment, long analysis time, high cost, low sensitivity, and skilled operators.…”

Biochemical Analysis

“…Separation of the enol and keto tautomers of aromatic pyruvic acids was accomplished on paper (317), as was also the separation of trace amounts of strontium and calcium in biological materials (118). High temperature paper chromatography served to determine lysine, arginine, and histidine (328). Glass paper chromatography was used for the determination of aldosterone in urine (337), of plasma cholesterol (345), and for the separation of glycerides of mixed fatty acid chain length (273)-the glass paper in this latter publication having been impregnated with silicic acid.…”

“…Except for the book by Martin and James (726), all current monographs on the general topic of chromatography (209,328,467,650,1097) have been written, characteristically, by more than two authors. This also applies to a comprehensive treatise on paper chromatography (437).…”