Determination of main taxoids in Taxus species by microwave‐assisted extraction combined with LC‐MS/MS analysis

Abstract

A method based on microwave-assisted extraction (MAE) has been developed for the determination of paclitaxel and five related taxoids, namely 10-deacetylbaccatin III (10-DAB III), cephalomannine, 10-deacetylpaclitaxel (10-DAT), 7-xyl-10- deacetylpaclitaxel (7-xyl-10-DAT), and 7-epi-10-deacetylpaclitaxel (7-epi-10-DAT) in Taxus species in this study. The influential parameters of the MAE procedure were optimized, and the optimal conditions were as follows: extraction solvent 80% ethanol solution, solid/liquid ratio 1:10 (g/mL), temperature 50 degrees C, and three extraction cycles, each cycle 10 min. The method validation for LC-MS/MS analysis was performed. The LOD and LOQ were 3.16-9.20 and 12.20-30.45 ng/mL, respectively. Repeatability and reproducibility for the six taxiods with RSD ranged from 2.78 to 3.85% and from 5.26 to 6.60%. The recoveries of the method for the six taxoids were 92.6-105.6%. The developed MAE-LC-MS/MS method was also successfully applied to determine the contents of six taxoids in different Taxus species.

Improving 10-deacetylbaccatin III-10-β-O-acetyltransferase catalytic fitness for Taxol production

“…Its content in yew trees is as much as 0.5% (dry weight of the bark)18. XDT content is even higher than that of Taxol in nursery cultivated yew trees1920. This analogue is usually discarded during Taxol extraction, causing both resource waste and potential environmental pollution.…”

Optimization of microwave‐assisted extraction followed by RP‐HPLC for the simultaneous determination of oleanolic acid and ursolic acid in the fruits of Chaenomeles sinensis

“…Major advantages of MAE include short extraction time, low energy requirement, high extraction efficiency and minimum degradation of target components 20. MAE has been of interest for the extraction of active compounds including flavonoids 21, phenolic compounds 22, 23, saponins 24 and other components 25, 26 from herbal medicines or TCMs. Up to now, the mechanism of extracting biologically active compounds from TCMs by microwave irradiation is still under study.…”

Simultaneous determination of seven taxoids in rat plasma by UPLC–MS/MS and pharmacokinetic study after oral administration of Taxus yunnanensis extracts

“…The methods used to determine the taxoids in plant extracts mainly include high-performance liquid chromatography (HPLC) with UV detection, enzyme-linked immunosorbent assays (ELISA) and high-speed countercurrent chromatography (HSCCC) [11,[16][17][18]. Recently, a few LC-MS methods were reported to determine paclitaxel and other taxoids in the extract of three Taxus species [8,[19][20][21]. However, no analytical method has been reported for the simultaneous determination of the main taxoids in plasma after oral administration of the extract of Taxus.…”