Determination of mangiferin in rat plasma by liquid–liquid extraction with UPLC–MS/MS

Han, Dandan; Chen, Chengjun; Zhao, Cong; Zhang, Yu; Tang, Xing

doi:10.1016/j.jpba.2009.07.021

Cited by 97 publications

(72 citation statements)

References 10 publications

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“…This phenomenon was consistent with the research results by Liu et al (2010). Han et al (2010) reported that mangiferin was poorly absorbed and its bioavailability was only 1.2%. In the present study, the plasma maximum concentration (C max ) and area under the concentration-time curve (AUC 0 -24 ) of mangiferin after oral administration to conventional rats were 715.04 Ϯ 600.14 ng/ml and 3799.68 Ϯ 1833.24 ng ⅐ h/ml, respectively.…”

Section: Resultssupporting

confidence: 93%

Metabolism and Pharmacokinetics of Mangiferin in Conventional Rats, Pseudo-Germ-Free Rats, and Streptozotocin-Induced Diabetic Rats

Liu

Pan

et al. 2012

Drug Metab Dispos

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ABSTRACT:To clarify the role of the intestinal flora in the absorption and metabolism of mangiferin and to elucidate its metabolic fate and pharmacokinetic profile in diabetic rats, a systematic and comparative investigation of the metabolism and pharmacokinetics of mangiferin in conventional rats, pseudo-germ-free rats, and streptozotocin (STZ)-induced diabetic rats was conducted. Forty-eight metabolites of mangiferin were detected and identified in the urine, plasma, and feces after oral administration (400 mg/kg). Mangiferin underwent extensive metabolism in conventional rats and diabetic rats, but the diabetic rats exhibited a greater number of metabolites compared with that of conventional rats. When the intestinal flora were inhibited, deglycosylation of mangiferin and sequential biotransformations would not occur. Pharmacokinetic studies indicated a 2.79-and 2.35-fold increase in the plasma maximum concentration and the area under the concentration-time curve from 0 to 24 h of mangiferin in diabetic rats compared with those for conventional rats, whereas no significant differences were observed between conventional rats and pseudo-germ-free rats. Further real-time quantitative reverse transcription-polymerase chain reaction results indicated that the multidrug resistance (mdr) 1a level in the ileum increased, whereas its level in the duodenum and the mdr1b mRNA levels in the duodenum, jejunum, and ileum decreased in diabetic rats compared with those in conventional rats. With regard to the pseudo-germ-free rats, up-regulated mdr1a mRNA levels and down-regulated mdr1b mRNA levels in the small intestines were observed. The diabetic status induced increased UDP-glucuronosyltransferase (UGT) 1A3, UGT1A8, UGT2B8, and sulfotransferase (SULT) 1A1 mRNA levels and decreased catechol-Omethyltransferase (COMT), UGT2B6, UGT2B12, and SULT1C1 mRNA levels. These results might partially explain the different pharmacokinetic and metabolic disposition of mangiferin among conventional and model rats.

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Section: Resultssupporting

confidence: 93%

Metabolism and Pharmacokinetics of Mangiferin in Conventional Rats, Pseudo-Germ-Free Rats, and Streptozotocin-Induced Diabetic Rats

Liu

Pan

et al. 2012

Drug Metab Dispos

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“…213 and 167, GAL and DPH) were selected in quadrupole 3 (Q3). The quantifier precursor to product ion (M+H) + transition of 288-to-213 m/z for GAL is shown in Figure 3 [31,32,34,35]. organic extraction solvent was used the P' for that solvent consisted of three main contributions, x e (interaction with ethanol -proton acceptors), x d (interaction with dioxane -proton donors) and x n (interaction with nitromethane -dipole-dipole interactions) that could be fine tuned for chromatographic separation integrity and extraction recovery selectivity.…”

Section: Mass Spectrometric Positive Ion Turbo Ionspray Mode Quantifimentioning

confidence: 99%

An Extraction Assay Analysis for Galanthamine in Guinea Pig Plasma and its Application to Nerve Agent Countermeasures

Steiner¹

2012

J Anal Bioanal Techniques

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Galanthamine hydrobromide (GAL HBr), approved material for treatment of mild to moderate Alzheimer's disease, is a centrally-acting reversible acetylcholinesterase inhibitor (AChEI) that is currently under evaluation as a therapeutic countermeasure against organophosphorus G-and V-Series nerve agents, which can induce rapid lethality in guinea pigs and humans. It has been shown that upon combination with atropine (ATR) and pyridine-2-aldoxime methochloride (2-PAM), a single dose of GAL administered before or soon after the acute exposure to a lethal dose of organophosphorus compounds can safely counteract toxicity in guinea pigs. To that end a new sample preparation extraction method analysis assay has been explored to enable future high-throughput, reproducible, and sensitive assays for the extraction of galanthamine in guinea pig plasma. Samples were prepared with diphenhydramine hydrochloride (DPH HCl) internal standard and recovered with 10 min liquid-liquid trichloromethane extractions. Samples were analyzed with a reversed phase liquid chromatographic column interfaced to a triple quadrupole mass spectrometer (LC/MS/MS) operating in the positive ion multiple reaction monitoring (MRM) Turbo Ionspray mode. Precursor to product ion (M+H) + transitions of 288-to-213 m/z and 256-to-167 m/z for GAL and DPH were observed, respectively. Sample run times of 1.50 min were achieved.

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“…The metabolism and pharmacokinetic profiles of mangiferin have recently been examined [16][17][18]. Pharmacokinetic studies have indicated that mangiferin exhibits poor oral bioavailability (1.2%) when orally administered with the isolated compound directly [19]. However, very few studies have been published on the pharmacokinetics and…”

Section: Introductionmentioning

confidence: 99%

The Pharmacokinetics and Metabolism of Neomangiferin, a Major Bioactive Component in Anemarrhena asphodeloides

Gao¹,

Liu²

2016

Med chem

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Determination of mangiferin in rat plasma by liquid–liquid extraction with UPLC–MS/MS

Cited by 97 publications

References 10 publications

Metabolism and Pharmacokinetics of Mangiferin in Conventional Rats, Pseudo-Germ-Free Rats, and Streptozotocin-Induced Diabetic Rats

Metabolism and Pharmacokinetics of Mangiferin in Conventional Rats, Pseudo-Germ-Free Rats, and Streptozotocin-Induced Diabetic Rats

An Extraction Assay Analysis for Galanthamine in Guinea Pig Plasma and its Application to Nerve Agent Countermeasures

The Pharmacokinetics and Metabolism of Neomangiferin, a Major Bioactive Component in Anemarrhena asphodeloides

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