Determination of Marbofloxacin residues in Rabbit Tissues by HPLC

Abstract

The present study was designed to evaluate marbofloxacin residues in different rabbit tissues after multiple intramuscular administrations. For that purpose, rabbits were divided into two groups; the first group (n=21) administered 2mg/kg marbofloxacin for five successive days, while the second group (n=3) were untreated and served as controls. th and 21 st day after the last dose post administration of the drug. Liver, kidneys, pectoral muscle and thigh muscles, spleen, heart, blood and lung from each rabbit were taken, extracted and marbofloxacin residues were analyzed using high-performance liquid chromatographic method with ultraviolet detection. Results indicated a widespread distribution of marbofloxacin in the most tested tissues. It remained within detectable level till the 5 th day in liver and serum while it continues till the 7 th in kidneys day following the last dose. Therefore, muscles of rabbits treated with marbofloxacin could be consumed safely following the 1 st day post treatment, while liver and kidneys could be consumed safely in the 3 rd day after treatment without any hazards on consumers as the residual level below the recommended MRL (150µg/kg).

Determination of antibiotics in meat samples using analytical methodologies: A review

“…Their extraction involves organic solvents such as ACN (Bozkurt et al, 2016;Iveta Pugajeva et al, 2018;H. Yu et al, 2019) combined with other organic solvents or aqueous solutions (Barreto et al, 2017;Chung et al, 2018;Gaolape, 2017;Hyung, Lee, & Kim, 2017;Moghadam et al, 2018;Pagani & Ibañez, 2019;Rasheed et al, 2017;Wen et al, 2020;, MeOH (Wang, Tong, Xu, & Zhang, 2018) containing acids solutions (Aziz Abdel, El-Nabtity, El Barawy, & Saleh, 2017;Ha, Chung, & Bae, 2016;, EDTA-McIlvaine (Lu et al, 2019) or ethanol (Chaowana & Bunkoed, 2019). Moreover, aqueous solutions containing sodium dodecyl sulfate (SDS; Peris-Vicente et al, 2017; Peris-Vicente, Iborra-Millet, Albiol-Chiva, Carda-Broch, & Esteve-Romero, 2019; Terrado-Campos, Tayeb-Cherif, Peris-Vicente, Carda-Boshc, & Esteve-Romero, 2017) or PBS (Sun et al, 2016) have been employed to extract Qs from meats.…”

“…Yu et al, 2019), dual-template molecularly imprinted polymer-stir bar sorptive extraction (DMI-SBSE; K. , DLLME (Moghadam et al, 2018) and hollow fiber-supported liquid membrane (Gaolape, 2017) have been used as purification and enrichment step before their quantification in meat samples. For this purpose, the use of LC with UV (Aziz Abdel et al, 2017;Moghadam et al, 2018;Sun et al, 2016;Wang et al, 2018;, FLD (Bozkurt et al, 2016;Peris-Vicente et al, 2017, 2019Terrado-Campos et al, 2017) or LC coupled to MS/MS detection (Barreto et al, 2017;Chung et al, 2018;Gaolape, 2017;Hyung et al, 2017;Lu et al, 2019;Pugajeva et al, 2018;Wen et al, 2020; H. Yu et al, 2019) have been described. However, others F I G U R E 5 Schematic fabrication process of Fe 3 O 4 @COF(TpBD)@Au-MPS and the MSPE procedure for the determination of FQs (reproduced with permission from Wen et al, 2020) Abbreviations: BD, benzidine; COF, covalent organic framework; FQs, fluoroquinolones; MPS, 3-mercaptopropanesulfonate; MSPE, magnetic solid phase extraction; NPs, nanoparticles; Tp, 1,3,5-triformylphloroglucinol methods based on UV-Vis (Rasheed et al, 2017), biochips (Ha et al, 2016) and fluorescence detection (Chaowana & Bunkoed, 2019;Pagani & Ibañez, 2019) have been also employed.…”