Determination of medullasin levels for the diagnosis of multiple sclerosis

Aoki, Yosuke; Saida, Takahiko; Nakano, Imaharu; Saito, Toyokazu; Ikeguchi, Kunihiko; Urabe, Takao; Nishiguchi, E.; Suzuki, Hidekazu; Takahashi, Kihito; Katsuragi, Hisashi; Mizuno, Yoshikuni

doi:10.1034/j.1600-0404.2000.102004218.x

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2006

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“…Medullasin activity in granulocytes was elevated in patients with various inflammatory diseases such as multiple sclerosis 17) , 18) and rheumatoid arthritis. 19)…”

Section: Discussionmentioning

confidence: 99%

Azurocidin induces elastinolytic activity of medullasin

Aoki

2006

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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Addition of azurocidin, a protein in granulocytes similar to serine proteases but has no protease activity because of replacement of the active serine residue by glycine, to the incubation mixture containing medullasin induced elastinolytic activity of medullasin. Both medullasin and human leukocyte elastase were already shown to have negligible elastinolytic activity (Aoki, Y. et al. J. Biochem. 114, 122, 1993). Elastinolytic activity of medullasin was induced dose-dependently by the addition of azurocidin. Medullasin activity determined by using apo-ornithine transaminase or casein as substrates or that by N-methoxy-succinyl-(Ala)2-Pro-Val-p-nitroanilide as substrate remained unchanged when azurocidin was added to the tube containing medullasin. Therefore, azurocidin is considered to cause an appearance of elastinolytic activity of medullasin without affecting the protease activity of it.

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“…Medullasin activity in granulocytes was elevated in patients with various inflammatory diseases such as multiple sclerosis 17) , 18) and rheumatoid arthritis. 19)…”

Section: Discussionmentioning

confidence: 99%

Azurocidin induces elastinolytic activity of medullasin

Aoki

2006

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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show abstract

Determination of medullasin levels for the diagnosis of multiple sclerosis

Abstract: This newly developed enzyme immunoassay method for medullasin is considered to be a useful paraclinical test for the diagnosis of multiple sclerosis.

Cited by 1 publication

References 6 publications

Azurocidin induces elastinolytic activity of medullasin

Azurocidin induces elastinolytic activity of medullasin

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