Determination of Memantine in Human Plasma by LC–MS–MS: Application to a Pharmacokinetic Study

Pan, Ryh‐Nan; Chian, Ting-Yi; Kuo, Benjamin Pei-Chung; Pao, Li-Heng

doi:10.1365/s10337-009-1231-8

Cited by 19 publications

(21 citation statements)

References 17 publications

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“…[6] High performance liquid chromatographic methods coupled with fluorescence detection have been reported in the literature for the quantification of MEM in rat plasma, [7,8] human plasma. [9][10][11] Determination of memantine in human plasma by liquid chromatography with mass spectrometry (LC-MS), [12][13][14][15] gas chromatography with mass spectrometry (GC-MS) [16] and micellar electro kinetic chromatography (MEKC) [17], extractive-Spectrophotometric [18] oxidation-reduction reaction-spectrophotometric [19] or Potentiometrically [20,21].…”

Section: Scheme 1 Structure Of Memantine Hydrochloride (Mem)mentioning

confidence: 99%

Spectrophotometric Determination of Alzheimer’s Drug, Memantine Hydrochloride in Biological Samples Using Ninhydrin and Ferric Chloride

Omara¹

2017

IOSR JAC

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Section: Scheme 1 Structure Of Memantine Hydrochloride (Mem)mentioning

confidence: 99%

Spectrophotometric Determination of Alzheimer’s Drug, Memantine Hydrochloride in Biological Samples Using Ninhydrin and Ferric Chloride

Omara¹

2017

IOSR JAC

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“…Similarly, for IS m/z 180.10 precursor ion to the m/z 110.10 was used for quantification purpose. As the earlier publications [6][7][8] have discussed extensively on fragmentation pattern of MEM and the data pertaining to this was not presented here.…”

Section: Optimization Of Liquid Chromatography and Mass Spectrometry mentioning

confidence: 99%

“…The mean terminal half-life (t ½ ) is around 60-80 h. MEM and its metabolites are primarily eliminated through urine (75-90%) and the remaining recovered through bile and feces. 4,5 Majority of the LC-MS/MS methods have been reported for estimation of MEM in clinical studies [6][7][8][9][10][11][12] and one method to support preclinical study. Among these methods the lowest reported lower limit of quantification (LLOQ) was 0.05 ng/ml using 100 µl plasma.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive LC-MS/MS Method for Determinationof Memantine in Rat Plasma: Application to Pharmacokinetic Studies in Rats

Suresh¹,

Mullangi²,

Kumar³

2014

IJPER

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Background: A rapid and highly sensitive assay method has been developed and validated for the estimation of memantine (MEM) in rat plasma using liquid chromatography coupled to tandem mass spectrometry with electro spray ionization in the positive-ion mode. Method validation was performed as per United States Food and Drug Administration (US FDA) guidelines. Methods: The assay procedure involves a simple liquid-liquid extraction of MEM and phenacetin (internal standard, IS) from rat plasma using methyl tert-butyl methyl ether. Chromatographic separation was achieved with 0.2% formic acid: acetonitrile (40:60, v/v) at a flow rate of 0.50 ml/min on an Atlantis dC18 column with a total run time 3.0 min. The MS/MS ion transitions monitored were 180.10 ® 163.30 for MEM and 180.10 ® 110.10 for IS. Results: The results met the acceptance criteria mentioned in the guidelines. The lower limit of quantitation achieved was 0.025 ng/ml and the linearity was observed from 0.025 to 220 ng/ml. The intra-and inter-day precision was in the range of 0.88-6.84 and 2.57-6.79%, respectively. Conclusions: An LC-ESI-MS/MS for the determination of MEM in rat plasma employing simple liquidliquid extraction was developed and validated with high sensitivity and selectivity. This novel method has been applied to a pharmacokinetic study in rats.

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“…Determination of memantine in human plasma by liquid chromatography with mass spectrometry (LC-MS) [9][10][11], gas chromatography with mass spectrometry (GC-MS) [11] and micellar electro kinetic chromatography (MEKC) [13], or Potentiometrically [14,15]. Though the above mentioned chromatographic methods are sensitive.…”

Section: Introductionmentioning

confidence: 99%

Extractive-Spectrophotometric Methods for Determination of Alzheimer’s Drug in Pharmaceutical Formulations and in Biological Samples Using Sulphonphthalein Acid Dyes

2016

Can Chem Trans

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A simple, accurate and highly sensitive spectrophotometric methods are proposed for the rapid and accurate determination of Memantine HCl (MEM) using Bromophenol blue (BPB), Bromocressol purple (BCP) and Methyl orange (MO). The developed methods involve formation of stable yellow colored chloroform extractable ion-associate complexes of the amino derivative (basic nitrogen) of the MEM with three sulphonphthalein acid dyes, namely; BPB, BCP and MO, in phthalate buffer of pH range (3.0-3.8). The ion-associates exhibit absorption maxima at 415, 410 and 422 nm for BPB, BCP and MO, respectively. MEM can be determined up to 1.1-12.5, 0.8-13.1 and 0.6-14 µg/mL, respectively. The effect of optimum conditions via pH, reagent concentration, time, and solvent were studied. The low relative standard deviation values indicate good precision and high recovery values. These methods have been successfully applied for the assay of MEM in pharmaceutical formulations. Statistical comparison of the results with the reference method shows excellent agreement and indicates no significant difference in accuracy and precision.

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Determination of Memantine in Human Plasma by LC–MS–MS: Application to a Pharmacokinetic Study

Cited by 19 publications

References 17 publications

Spectrophotometric Determination of Alzheimer’s Drug, Memantine Hydrochloride in Biological Samples Using Ninhydrin and Ferric Chloride

Spectrophotometric Determination of Alzheimer’s Drug, Memantine Hydrochloride in Biological Samples Using Ninhydrin and Ferric Chloride

Highly Sensitive LC-MS/MS Method for Determinationof Memantine in Rat Plasma: Application to Pharmacokinetic Studies in Rats

Extractive-Spectrophotometric Methods for Determination of Alzheimer’s Drug in Pharmaceutical Formulations and in Biological Samples Using Sulphonphthalein Acid Dyes

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