Determination of mercury in fish by photochemical vapor generation graphite furnace atomic absorption spectrometry

Potes, Mariana da Luz; Kolling, Leandro; Jesus, Alexandre de; Dessuy, Morgana B.; Vale, Maria Goreti R.; Silva, Márcia Messias da

doi:10.1039/c6ay02342h

Cited by 16 publications

(2 citation statements)

References 35 publications

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“…Currently, the classical analytical methods used for detecting mercury are given as atomic fluorescence spectrometry 4 (AFS), inductively coupled plasma mass spectrometry 5 (ICP-MS), and atomic absorption spectrometry 6 (AAS). Even these traditional methods exhibit high accuracy and sensitivity, but they require expensive equipment and trained professionals.…”

Section: ■ Introductionmentioning

confidence: 99%

Engineering the Ultrasensitive Visual Whole-Cell Biosensors by Evolved MerR and 5′ UTR for Detection of Ultratrace Mercury

Zhu,

Chen,

Cai

et al. 2023

Environ. Sci. Technol.

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The existing mercury whole-cell biosensors (WCBs, parts per billion range) are not able to meet the real-world requirements due to their lack of sensitivity for the detection of ultratrace mercury in the environment. Ultratrace mercury is a potential threat to human health via the food chain. Here, we developed an ultrasensitive mercury WCB by directed evolution of the mercury-responsive transcriptional activator (MerR) sensing module to detect ultratrace mercury. Subsequently, the mutant WCB (m4-1) responding to mercury in the parts per trillion range after 1 h of induction was obtained. Its detection limit (LOD) was 0.313 ng/L, comparable to those of some analytical instruments. Surprisingly, the m4-1 WCB also responded to methylmercury (LOD = 98 ng/L), which is far more toxic than inorganic mercury. For more convenient detection, we have increased another green fluorescent protein reporter module with an optimized 5′ untranslated region (5′ UTR) sequence. This yields two visual WCBs with an enhanced fluorescence output. At a concentration of 2.5 ng/L, the fluorescence signals can be directly observed by the naked eye. With the combination of mobile phone imaging and image processing software, the 2GC WCB provided simple, rapid, and reliable quantitative and qualitative analysis of real samples (LOD = 0.307 ng/L). Taken together, these results indicate that the ultrasensitive visual whole-cell biosensors for ultratrace mercury detection are successfully designed using a combination of directed evolution and synthetic biotechnology.

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Section: ■ Introductionmentioning

confidence: 99%

Engineering the Ultrasensitive Visual Whole-Cell Biosensors by Evolved MerR and 5′ UTR for Detection of Ultratrace Mercury

Zhu,

Chen,

Cai

et al. 2023

Environ. Sci. Technol.

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“…16 as an alternate method of vapour generation for mercury analysis using AFS. Since then, PVG has been applied to total mercury measurements using UV with formic acid [17][18][19][20][21] and acetic acid 22 , eg. utilizing matrix assisted photochemical vapour generation to determine total mercury in white vinegar.…”

Section: Introductionmentioning

confidence: 99%

Determination of methylmercury using liquid chromatography – photochemical vapour generation – atomic fluorescence spectroscopy (LC-PVG-AFS): a simple, green analytical method

Lancaster

Brombach²,

Corns³

et al. 2019

J. Anal. At. Spectrom.

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Rapid and Low Cost Determination of Total Mercury in Cat Foods by Photochemical Vapor Generation Coupled to Atomic Absorption Spectrometry

Silva

Nobre

Lopes

2020

Biol Trace Elem Res

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Determination of mercury in fish by photochemical vapor generation graphite furnace atomic absorption spectrometry

Abstract: Hg was determined in fish samples treated with TMAH, using n-propanol to assist the photochemical vapor generation.

Cited by 16 publications

References 35 publications

Engineering the Ultrasensitive Visual Whole-Cell Biosensors by Evolved MerR and 5′ UTR for Detection of Ultratrace Mercury

Engineering the Ultrasensitive Visual Whole-Cell Biosensors by Evolved MerR and 5′ UTR for Detection of Ultratrace Mercury

Determination of methylmercury using liquid chromatography – photochemical vapour generation – atomic fluorescence spectroscopy (LC-PVG-AFS): a simple, green analytical method

Rapid and Low Cost Determination of Total Mercury in Cat Foods by Photochemical Vapor Generation Coupled to Atomic Absorption Spectrometry

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