Determination of mercury in natural waters and effluents by flameless atomic absorption spectrophoto- metry

Omang, Sverre H.

doi:10.1016/s0003-2670(01)82101-2

Cited by 100 publications

(17 citation statements)

References 5 publications

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“…6 Tryptophan, 0.1% Casamino acids) containing mercurial compounds of 10 mM, 1 mM, 0.1 mM of mercury chloride (MC) or 5 mM, 0.5 mM, 0.05 mM of phenylmercury acetate (PMA) and were incubated at 37 °C for some hours. For the luciferase assay, an aliquot (290 µl) of culture was transferred to a luminometric cuvette and the biological luminescence reaction was started by adding an aliquot (10 µl) of 10 % 1-decanal (v/v, dissolved in ethanol).…”

Section: Bio-luminescence Assaysmentioning

confidence: 99%

“…Because the mercurial compounds tend to be accumulated through biological food chain systems, detection and removal of those bio-affecting pollutants at a low level of contamination are the major goals to control the mercury poisoning problems. For the detection of the mercurial compounds, physicochemical analysis methods such as atomic absorption spectrophotometry [6] and cold vapour atomic fluorometry [7] have been developed. Besides the laborious preparation of samples, however, these procedures are not useful in detecting mercurial compounds and distinguishing those bioaffecting forms from non-bioaffecting inert forms or organomercurial from inorganic mercurial forms.…”

Section: Introductionmentioning

confidence: 99%

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Bioluminescence Biosensor for the Detection of Organomercury Contamination

Endo

Yamagata

Narita

et al. 2003

Acta Biotechnologica

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To selectively detect organomercurial compounds in the environment, in this study a bioluminescence biosensor for organomercurials was developed using a bacterial gene expression system for the mercury resistance determinant. merB 3 -Luciferase (mer-lux) transcriptional fusion plasmids pHYΒ3Lux and pHY∆Β3Lux were constructed to evaluate the gene expression system with a new organomercury lyase gene merB 3 from Bacillus megaterium strain MB1, which is resistant to a broad spectrum of mercury compounds, and with its 3'end-deleted defective merB 3 , respectively. Another plasmid, pGR 1 A, encoding an operator/promoter sequence, merR 1 , merE, merT, merP and merA from the same bacterial strain was constructed and used as a transacting gene expression vector which combines the gene expression vector of mer-lux transcriptional fusion plasmids in the same Escherichia coli cells. The transformants that carried a set of the two plasmids were used as biological sensors for the detection of organomercurials. Transformant (E. coli DH5α/pHYB3Lux, pGR1A) is available to distinguish the organomercury from inorganic mercury, since inorganic mercurials can induce the bioluminescence of both the bacterial strain lines with pHY∆B3Lux and pHYB3Lux, whereas organomercurials can only induce the bioluminescence of the lines with pHYB3Lux. These experimental results showed that the transformant with a merB 3 -defective fusion plasmid, and the gene expression vector responded to only mercury chloride. On the other hand, the transformant with an intact merB 3 fusion plasmid and the gene expression vector responded to mercury chloride and all organomercurials tested in the study. The sensor system responded to the existence of the phenyl mercury acetate of 50 nanomolar and was more sensitive than that of inorganic mercury (100 nanomolar). The result also indicated the capability of the system to detect bio-affecting inorganic mercury from several hundred nanomolar to several ten micromolar.

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Section: Bio-luminescence Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioluminescence Biosensor for the Detection of Organomercury Contamination

Endo

Yamagata

Narita

et al. 2003

Acta Biotechnologica

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“…Blanks and standard solutions were treated in a similar way. In the analysis of Hg, the vapour generation method of Omang (1970) was employed ; mercury hydride was generated by the addition of tin(II) chloride solution into the water samples .…”

Section: Analysis Of Water Samplesmentioning

confidence: 99%

The determination of heavy metals in water, suspended materials and sediments from Langat River, Malaysia

Sarmani

1989

Hydrobiologia

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The distributions of heavy metals in the Langat River were studied for a period of six months between September 1984 and February 1985 . Heavy metals such as arsenic, cadmium, cerium, cobalt, chromium, caesium, lanthanum, rubidium, antimony, scandium, thorium and zinc were determined in water, suspended materials and sediment samples from the Langat River by neutron activation and atomic absorption spectrometry . The concentrations of arsenic, cadmium, cerium, cobalt, scandium, antimony, and zinc were generally highest in the suspended materials, whereas the concentrations of chromium, rubidium and thorium were always highest in the sediments : Arsenic concentrations in the river were slightly higher than the natural concentration, while other elements were generally at their natural concentration levels . The use of arsenical herbicides in plantations along the river could be a source of arsenic pollution . 233

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“…To prevent the environmental mercury poisoning incidents, measuring and monitoring of mercury in the environment are necessitated. Classical methods such as atomic absorption spectrometry, 1) cold-vapor atomic fluorescence detection 2) and inductively coupled plasma mass spectrometry 1,3) are widely used due to their sensitive. However, these methods need sophisticated instrumentation and highly skilled personnel, complicated sample preparation and a long measuring time period.…”

Section: Introductionmentioning

confidence: 99%