Determination of mercury(II) by its inhibitory effect on the enzymic reaction of ethanol oxidation using flow injection

Abstract

A flow injection procedure is described for the determination of Hg*+, based on its non-competitive inhibition of the catalytic effect of alcohol dehydrogenase o n the oxidation of ethanol b y the coenzyme nicotinamide adenine dinucleotide. Mercury can be determined within the range 0.5-20 ppm with a relative standard deviation of I-1.5% and at a sampling rate of 120-150 h-1. Only Ag+ was observed t o interfere.

Kinetic Determination of Mercury(II) at Trace Level from Its Catalytic Effect on a Ligand Substitution Process

“…Most of these involve oxidation reactions [11][12][13][14][15][16][17][18][19], while references based on ligand substitution reactions are scarce [20][21][22][23][24]. In many cases, the inhibitory effect of mercury(II) on catalytic indicator systems [11][12][13][14][15] or of the catalytic action of mercury(II) on slow redox processes [16][17][18][19] has been utilized to develop CKMs for the determination of mercury(II). Pirvutoiu et al [13] developed a highly sensitive method for the determination of mercury(II); however, the method uses a costly enzyme as a substrate and a hard-to-find thermometric detection system.…”

Kinetic‐Catalytic and Spectrophotometric Determination of Hg(II) Using its Catalytic Effect on Ligand Substitution Reaction between Hexacyanoferrate(II) and Pyrazine

“…These methods have been found to be cheaper and more reliable alternative to the instrumental methods of analysis for trace determinations. [15][16][17][18][19][20][21][22][23] On contrary, the references on mercury(II) determination based on ligand substitution reactions are limited. [15][16][17][18][19][20][21][22][23] On contrary, the references on mercury(II) determination based on ligand substitution reactions are limited.…”

“…14 Numerous indicator reactions have been exploited for trace determination of mercury by kinetic methods and majority of them involve oxidation reactions. [24][25][26][27][28] In many cases inhibitory effect on catalytic indicator system [13][14][15][16][17][18][19] or catalytic action on slow redox processes [20][21][22][23] has been used to develop a kinetic-catalytic method for trace determination of mercury(II). [24][25][26][27][28] In many cases inhibitory effect on catalytic indicator system [13][14][15][16][17][18][19] or catalytic action on slow redox processes [20][21][22][23] has been used to develop a kinetic-catalytic method for trace determination of mercury(II).…”

“…-Effect of diverse ions on the determination of 5.07 ng mL -1 of Hg(II) using A15 Calibration graph under the experimental conditions given inTABLE 2. Na + , K + , Li + , Bi 3+ , Pb 2+ , Co 2+ Ag + , Fe 2+ , Mn 2+ , Mg 2+ , Pd 2+ , 998.10…”