Determination of mercury (thimerosal) in vaccines using different oxidants and cold vapor atomic fluorescence spectrometry in dilute acids

Abstract

A simple and fast method was developed for the determination of thimerosal (TH) in vaccines based on decomposition through the action of oxidizing systems of the organic mercury compound to...

Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages

“…To some extent the reports of Gawor et al, 211 de Oliveira et al 50 and Vojtek et al 32 support Timerbaev's observations. Gawor et al 211 assert that 20-25% of all commercial pharmaceuticals are uorinated compounds, some F-containing compounds are harmless to health, but some are toxic and there are few available procedures for measuring concentrations of F in biological samples.…”

“…The study by Fu et al, 66 demonstrated greater sensitivity and lower background with this gas mixture compared with the more commonly used NH 3 /He gases. The LODs for Ti in human serum ranged from 0.78 ng L À1 for 48 Ti to 7.20 ng L À1 for less abundant 50 Ti and RSDs were between 2.0 and 4.2%. Results were veried by comparison with SF-ICP-MS measurements.…”

“…The assay parameters were reported as LOD: 0.03 mg g À1 , LOQ: 0.08 mg g À1 , linearity: r ¼ 0.9998, accuracy: 86-110%, precision: <10%, extraction recovery: 89-110%. Chicken tissue samples enriched with 53 Cr III and 50 Cr VI isotopes were analysed to investigate possible species interconversion during the procedure. The Cr III was stable during all steps of developed analytical approach, while about 30% of added Cr VI was reduced by the sample matrix components.…”

“…The system was used to investigate the kinetics of interactions between Cr III and Cr VI with serum constituents. Human serum samples were doubly spiked with enriched 53 Cr III and 50 Cr VI and incubated at 37 C. Samples were taken for chromatographic separation and detection at intervals from 5 min to 48 h. It was seen that 53 Cr III rapidly interacted with transferrin and that by 48 h more than 90% was bound, with the remainder associated with albumin. The 50 Cr VI was reduced to 50 Cr III and was bound to transferrin while 20% remained as 50 Cr VI .…”

Atomic spectrometry update: review of advances in the analysis of metals, chemicals and materials

“…A simple and rapid method for determining thimerosal (also known as thiomersal) in vaccines was developed by de Oliveira et al 81 It is added as a preservative, but being an organomercury compound, is toxic. The method of detection was cold vapour-atomic fluorescence spectrometry.…”