Determination of methionine-enkephalin and leucine-enkephalin by LC-MS in human plasma: Study of pre-analytical stability

Özalp, Aliye; Barroso, Begoña; Meijer, John; Beld, Cas van den

doi:10.1016/j.ab.2018.07.001

Cited by 10 publications

(4 citation statements)

References 19 publications

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“…Fatty acid examples include 3-hydroxydodecanoic acid ( m/z , 199.169; RT, 7.10 min; medium-chain fatty acid associated with fatty acid metabolic disorders, potentially acquired from the microbial genera Pseudomonas , Moraxella , and Acinetobacter [ 65 , 66 ]), and palmitoleic acid ( m/z , 237.001; RT, 6.42 min; fatty acid commonly found in human adipose tissue; also acquired in diet from human breast milk [ 67 ]). Additional metabolites include cholesterol ( m/z , 369.352; RT, 10.5 min; essential sterol found in animals [( 6 ]), methionine ( m/z , 105.058; RT, 0.33 min; amino acid), and leucine enkephalin ( m/z , 336.192; RT, 3.21 min; peptide naturally produced in animal brains, including humans [ 6 , 68 ]). While a number of the shared metabolite features listed above provide key biological functions, some metabolites appear to be derived from dietary sources.…”

Section: Resultsmentioning

confidence: 99%

Untargeted Fecal Metabolomic Analyses across an Industrialization Gradient Reveal Shared Metabolites and Impact of Industrialization on Fecal Microbiome-Metabolome Interactions

et al. 2022

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Section: Resultsmentioning

confidence: 99%

Untargeted Fecal Metabolomic Analyses across an Industrialization Gradient Reveal Shared Metabolites and Impact of Industrialization on Fecal Microbiome-Metabolome Interactions

et al. 2022

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“…To acquire the absolute concentration of neuropeptides, (a) a calibration curve is required (Chung‐Davidson et al, 2020; Schmerberg et al, 2015; Song & Liu, 2008; Wang et al, 2014); (b) a synthetic, isotopic internal standard, also known as an AQUA peptide is added (Bozzacco et al, 2011; Ozalp et al, 2018; Salem et al, 2018); or (c) a peptide standard similar to the peptide of interest (Dong et al, 2018) to be used as a proxy. Several software packages assist in processing these large datasets, including commercial software packages (SIEVE, PEAKS, Proteome Discoverer, etc.)…”

Section: Advances In Quantitationmentioning

confidence: 99%

Recent advances in mass spectrometry analysis of neuropeptides

Phetsanthad

et al. 2021

Mass Spectrometry Reviews

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Due to their involvement in numerous biochemical pathways, neuropeptides have been the focus of many recent research studies. Unfortunately, classic analytical methods, such as western blots and enzyme‐linked immunosorbent assays, are extremely limited in terms of global investigations, leading researchers to search for more advanced techniques capable of probing the entire neuropeptidome of an organism. With recent technological advances, mass spectrometry (MS) has provided methodology to gain global knowledge of a neuropeptidome on a spatial, temporal, and quantitative level. This review will cover key considerations for the analysis of neuropeptides by MS, including sample preparation strategies, instrumental advances for identification, structural characterization, and imaging; insightful functional studies; and newly developed absolute and relative quantitation strategies. While many discoveries have been made with MS, the methodology is still in its infancy. Many of the current challenges and areas that need development will also be highlighted in this review.

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“…Fatty acid examples include 3-hydroxydodecanoic acid ( m/z 199.169; RT 7.10 min; medium chain fatty acid associated with fatty acid metabolic disorders, potentially acquired from the microbial genera Pseudomonas, Moraxella, and Acinetobacter 53, 54 ) and palmitoleic acid ( m/z 237.001; RT 6.42 min; fatty acid commonly found in human adipose tissue and associated with obesity 55 ; also acquired in diet from human breast milk 56 ). Additional metabolites include cholesterol ( m/z 369.352; RT 10.5 min; essential sterol found in animals 6 ), methionine ( m/z 105.058; RT 0.33 min; amino acid), and leucine enkephalin ( m/z 336.192; RT 3.21 min; peptide naturally produced in animal brains, including humans 6, 57 ). While a number of these shared metabolite features listed above provide key biological functions, some metabolites appear to be derived from dietary sources.…”

Section: Manuscriptmentioning

confidence: 99%

The Core Human Fecal Metabolome

Haffner

Katemauswa

Kagoné

et al. 2021

Preprint

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Among the biomolecules at the center of human health and molecular biology is a system of molecules that define the human phenotype known as the metabolome. Through an untargeted metabolomic analysis of samples from Africa and the Americas, the birthplace and the last continental expansion of our species, we present the characterization of the core human fecal metabolome. The majority of detected metabolite features were ubiquitous across populations, despite any geographic, dietary, or behavioral differences. Such shared metabolite features included hyocholic acid and cholesterol. However, any characterization of the core human fecal metabolome is insufficient without exploring the influence of industrialization. Here we show chemical differences along an industrialization gradient, where the degree of industrialization correlates with metabolomic changes. We identified differential metabolite features like leucyl-leucine dipeptides and urobilin as major metabolic correlates of these behavioral shifts. Our results indicate that industrialization significantly influences the human fecal metabolome, but diverse human lifestyles and behavior still maintain a core human fecal metabolome. This study represents the first characterization of the core human fecal metabolome through untargeted analyses of populations along an industrialization gradient.

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Determination of methionine-enkephalin and leucine-enkephalin by LC-MS in human plasma: Study of pre-analytical stability

Cited by 10 publications

References 19 publications

Untargeted Fecal Metabolomic Analyses across an Industrialization Gradient Reveal Shared Metabolites and Impact of Industrialization on Fecal Microbiome-Metabolome Interactions

Untargeted Fecal Metabolomic Analyses across an Industrialization Gradient Reveal Shared Metabolites and Impact of Industrialization on Fecal Microbiome-Metabolome Interactions

Recent advances in mass spectrometry analysis of neuropeptides

The Core Human Fecal Metabolome

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