Determination of methionine in pharmaceuticals after chromatographic separation

Buhl, Franciszek; Gałkowska, Monika

doi:10.1556/jpc.19.2006.5.12

Cited by 10 publications

(5 citation statements)

References 15 publications

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“…In the literature, there have been reported many spetrophotometric methods for the determination of NAC. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] One group of described methods involves redox reactions with NAC in which a colored compound is formed or decomposed during determination. Among the described indirect spectrophotometric procedures one of the most common approaches to the determination of NAC is based on the coupled redox complexation reaction.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands

Kukoc-Modun¹,

Tsikas²,

Kraljević³

et al. 2017

Croat. Chem. Acta

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Three simple, sensitive and robust kinetic spectrophotometric methods for the determination of a novel lipophilic thiol compound, N-acetyl-L-cysteine ethyl ester (NACET), have been developed and validated. The methods are based on the reduction of Cu(II)-ligand complex to Cu(I)-ligand complex with the analyte. Studied ligands were neocuproine, bicinchoninic acid (BCA) and bathocuproine disulfonic acid (BCS). The development of chromogenic complexes was followed using kinetic setup with spectrophotometric detection at 458, 562 and 483 nm for the reactions of NACET with neocuproine, BCA and BCS, respectively. The calculated reaction orders with respect to NACET concentration were found to be 1.07, 1.01, 1.07, respectively, thus confirming a first order of reaction. The initial rate and fixed time methods were utilized for constructing calibration curves. Assays limits of detection were 1.4 × 10 -7 , 3.2 × 10 −7 and 6.0 × 10 −8 mol L -1 , respectively. The analytical performance of the methods, in terms of accuracy and precision, was established.

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Section: Introductionmentioning

confidence: 99%

Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands

Kukoc-Modun¹,

Tsikas²,

Kraljević³

et al. 2017

Croat. Chem. Acta

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“…1 The European Pharmacopeia recommended iodimetric titration for pharmaceutical formulations analysis. 2 Other reported methods described in the literature comprise: titrimetry, 3,4 spectrophotometry, [5][6][7][8][9] fluorimetry, [10][11][12] chromatography, 13,14 potentiometry, 15 conductometry 16 and voltametry [17][18][19] for the determination of NAC in pure form, in dosage forms and in biological samples.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine Based on the Reduction of Copper(II)-neocuproine Reagent

Radić¹,

Kukoc-Modun²,

Biocic³

2013

Croat. Chem. Acta

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“…In addition, it is being studied and utilized for curing HIV infection, cancer and heart disease. 1 Several analytical methods have been reported for NAC determination in pharmaceutical formulations, including spectrophotometry, [2][3][4][5][6][7] fluorimetry, 8,9 chromatography 10,11 and electrochemistry. 12,13 Some of the methods described above are not simple for direct application in a large-scale routine analysis and require expensive or sophisticated instruments.…”

Section: Introductionmentioning

confidence: 99%

Flow-injection Spectrophotometric Determination of N-acetyl-L-cysteine Based on Coupled Redox-complexation Reaction

2011

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Abstract. A flow-injection spectrophotometric method is developed for determination of N-acetyl-Lcysteine (NAC) in pharmaceutical formulations. The method is based on coupled redox-complexation reaction, the first step of which is the reduction of Fe III by NAC; the second one includes the complexation of Fe . The analytical frequency was 60 h -1 . Usual excipients used as additives in pharmaceuticals do not interfere with the analysis of NAC. The proposed method is simple, rapid, sensitive, accurate and precise (repeatability RSD 0.29 %, n = 10), and can be applied for determination of NAC in pharmaceutical formulations up to nanomole quantity. (doi: 10.5562/cca1753)

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Determination of methionine in pharmaceuticals after chromatographic separation

Cited by 10 publications

References 15 publications

Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands

Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands

Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine Based on the Reduction of Copper(II)-neocuproine Reagent

Flow-injection Spectrophotometric Determination of N-acetyl-L-cysteine Based on Coupled Redox-complexation Reaction

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