Determination of Methocarbamol in Equine Serum and Urine by High-Performance Liquid Chromatography with Ultraviolet Detection and Atmospheric Pressure Ionization-Mass Spectrometric Confirmation*

Koupai-Abyazani, Mohammed R.; Esaw, Barbara; Laviolette, Barbara

doi:10.1093/jat/21.4.301

Cited by 17 publications

(5 citation statements)

References 8 publications

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“…Its pharmacodynamic effects following a single intravenous pre-anesthetic dose to horses have been studied briefly, and high doses (>100 mg/kg) are well tolerated (Hubbell et al, 1980). However, there are no reports of its disposition following the administration of MCBL to horses, although the metabolism of MCBL to GGE and the excretion of GGE in urine after oral and intravenous administration of MCBL to horses have been known for several decades (Muir et al, 1984;Koupai-Abyazani et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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The pharmacokinetics of methocarbamol and guaifenesin after single intravenous and multiple‐dose oral administration of methocarbamol in the horse

Rumpler

Colahan

Samś

2013

Vet Pharm & Therapeutics

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A simple LC/MSMS method has been developed and fully validated to determine concentrations and characterize the concentration vs. time course of methocarbamol (MCBL) and guaifenesin (GGE) in plasma after a single intravenous dose and multiple oral dose administrations of MCBL to conditioned Thoroughbred horses. The plasma concentration-time profiles for MCBL after a single intravenous dose of 15 mg/kg of MCBL were best described by a three-compartment model. Mean extrapolated peak (C0 ) plasma concentrations were 23.2 (± 5.93) μg/mL. Terminal half-life, volume of distribution at steady-state, mean residence time, and systemic clearance were characterized by a median (range) of 2.96 (2.46-4.71) h, 1.05 (0.943-1.21) L/kg, 1.98 (1.45-2.51) h, and 8.99 (6.68-10.8) mL/min/kg, respectively. Oral dose of MCBL was characterized by a median (range) terminal half-life, mean transit time, mean absorption time, and apparent oral clearance of 2.89 (2.21-4.88) h, 2.67 (1.80-2.87) h, 0.410 (0.350-0.770) h, and 16.5 (13.0-20) mL/min/kg. Bioavailability of orally administered MCBL was characterized by a median (range) of 54.4 (43.2-72.8)%. Guaifenesin plasma concentrations were below the limit of detection in all samples collected after the single intravenous dose of MCBL whereas they were detected for up to 24 h after the last dose of the multiple-dose oral regimen. This difference may be attributed to first-pass metabolism of MCBL to GGE after oral administration and may provide a means of differentiating the two routes of administration.

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Section: Introductionmentioning

confidence: 99%

“…Several investigators have described the detection of MCBL or GGE in plasma or serum (Hubbell et al, 1980;Weng et al, 1994;Koupai-Abyazani et al, 1997;Zha & Zhu, 2010). Naidong et al (1994) described a LC/UV method for both compounds.…”

Section: Introductionmentioning

confidence: 99%

The pharmacokinetics of methocarbamol and guaifenesin after single intravenous and multiple‐dose oral administration of methocarbamol in the horse

Rumpler

Colahan

Samś

2013

Vet Pharm & Therapeutics

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“…The United States Pharmacopoeia (USP) recommends a spectrophotometric method for the determination of methocarbamol raw material and high‐performance liquid chromatography (HPLC) for its tablets. Several analytical methods have been reported for the determination of methocarbamol in tablets and human plasma such as spectrofluorimetry , spectrophotometry , electrochemical method , H 1 ‐NMR spectroscopy , GC , HPLC and supercritical fluid chromatography .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of methocarbamol and aspirin by RP‐HPLC using fluorescence detection with time programming: its application to pharmaceutical dosage form

2012

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A new simple, rapid and sensitive reversed‐phase liquid chromatographic method was developed and validated for the simultaneous determination of methocarbamol (MET) and aspirin (ASP) in their combined dosage form. The separation of these compounds was achieved within 6.0 min on a CLC Shim‐pack C8 column (250 × 4.6 mm, 5 µm particle size) using isocratic mobile phase consisting of acetonitrile and 0.02 M dihydrogenphosphate buffer (30:70, v/v) at pH = 5.0. The analysis was performed at a flow rate of 1.0 mL/min with fluorescence detection at 277/313 nm for MET and 298/410 nm for ASP using real‐time programming. The selectivity, linearity of calibration, accuracy, inter‐ and intra‐day precision and recovery were examined as parts of the method validation. The concentration–response relationship was linear over concentration ranges of 0.02‐0.20 and 0.02‐0.40 µg/mL for MET and ASP, respectively, with a limit of detection of 6 and 32 ng/mL for MET and ASP, respectively. The proposed method was successfully applied for the analysis of both MET and ASP in prepared tablets with average recoveries of 99.88 ± 0.65% for MET and 100.44 ± 0.78% for ASP. The results were favourably compared to those obtained by a reference method. Copyright © 2012 John Wiley & Sons, Ltd.

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“…Koupai-Abyazani et al applied HPLC with UV detection to determine the methocarbamol in equine serum and confirmed with LC-MS. The extraction method needed two steps; enzyme hydrolysis followed by liquid-liquid extraction [10].…”

Section: Introductionmentioning

confidence: 99%

Determination of methocarbamol concentration in human plasma by high performance liquid chromatography–tandem mass spectrometry

Zha

Zhu

2010

Journal of Chromatography B

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Determination of Methocarbamol in Equine Serum and Urine by High-Performance Liquid Chromatography with Ultraviolet Detection and Atmospheric Pressure Ionization-Mass Spectrometric Confirmation*

Cited by 17 publications

References 8 publications

The pharmacokinetics of methocarbamol and guaifenesin after single intravenous and multiple‐dose oral administration of methocarbamol in the horse

The pharmacokinetics of methocarbamol and guaifenesin after single intravenous and multiple‐dose oral administration of methocarbamol in the horse

Simultaneous determination of methocarbamol and aspirin by RP‐HPLC using fluorescence detection with time programming: its application to pharmaceutical dosage form

Determination of methocarbamol concentration in human plasma by high performance liquid chromatography–tandem mass spectrometry

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