Determination of methotrexate in spiked human blood serum using multi-frequency electrochemical immittance spectroscopy and multivariate data analysis

Phal, Sereilakhena; Lindholm-Sethson, Britta; Geladi, Paul; Shchukarev, Andrey; Tesfalidet, Solomon

doi:10.1016/j.aca.2017.08.034

Cited by 20 publications

(11 citation statements)

References 37 publications

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“…These methods are restricted due to their high cost and complexity of the operation. Besides these classical methods, electrochemical methods are also developed for the analysis of MTX [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. Electroanalytical methods are low cost, simple, selective, sensitive, very fast in response, and easy-to-handle, on-spot measurements.…”

Section: Introductionmentioning

confidence: 99%

“…This is now possible due to the manipulation of materials at the atomic level because of revolutionary advancements in the field of nanotechnology. For this purpose, intensive efforts are used to fabricate earth-abundant and low-cost metal oxide nanostructures such as NiO, CuO, TiO 2 , ZnO, and their hybrid structures with enhanced electrochemical activities [ 35 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ]. Nanostructured materials offer a high surface area for the sensing applications; thus, different morphologies at the nano level have shown outperforming properties in sensing electronics and optoelectronics applications [ 44 , 45 , 46 ].…”

Section: Introductionmentioning

confidence: 99%

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Facile Electrochemical Determination of Methotrexate (MTX) Using Glassy Carbon Electrode-Modified with Electronically Disordered NiO Nanostructures

et al. 2021

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Recently, the oxidative behavior of methotrexate (MTX) anticancer drug is highly demanded, due to its side effects on healthy cells, despite being a very challenging task. In this study, we have prepared porous NiO material using sodium sulfate as an electronic disorder reagent by hydrothermal method and found it highly sensitive and selective for the oxidation of MTX. The synthesized NiO nanostructures were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD) techniques. These physical characterizations delineated the porous morphology and cubic crystalline phase of NiO. Different electrochemical approaches have been utilized to determine the MTX concentrations in 0.04 M Britton–Robinson buffer (BRB) at pH 2 using glassy carbon electrode (GCE)-modified with electronically disordered NiO nanostructures. The linear range for MTX using cyclic voltammetry (CV) was found to be from 5 to 30 nM, and the limit of detection (LOD) and limit of quantification (LOQ) were 1.46 nM and 4.86 nM, respectively, whereas the linear range obtained via linear sweep voltammetry (LSV) was estimated as 15–90 nM with LOD and LOQ of 0.819 nM and 2.713 nM, respectively. Additionally, amperometric studies revealed a linear range from 10 to70 nM with LOD and LOQ of 0.1 nM and 1.3 nM, respectively. Importantly, MTX was successfully monitored in pharmaceutical products using the standard recovery method. Thus, the proposed approach for the synthesis of active metal oxide materials could be sued for the determination of other anticancer drugs in real samples and other biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Facile Electrochemical Determination of Methotrexate (MTX) Using Glassy Carbon Electrode-Modified with Electronically Disordered NiO Nanostructures

et al. 2021

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“…The electroanalytical methods have more preference due to their excellent sensitivity, selectivity, facile features, inexpensive nature, quick in response and very suitable for on spot detection. For this purpose, various modi ed electrodes have been fabricated for the electroanalysis of MTX [14][15][16][17][18][19][20][21][22][23][24][25][26]. At the present, the scientists and researchers are using variety of compounds in electroanalytical methods and investigating their activity in different conditions such as clinical samples.…”

Section: Introductionmentioning

confidence: 99%

Polyaniline as a Sacrificing Template for the Synthesis of Ultra-small Co3O4 Nanoparticles for the Sensitive and Selective Detection of Methotrexate (MTX)

Qureshi

Tahira

Aljadoa

et al. 2021

Preprint

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The successful monitoring of the anticancer drugs using nanostructured materials is very important but very challenging task. Beside this, uniform and ultra-small size of metal oxide nanoparticles is highly needed in order to enhance the catalytic activity which could result into the development of sensitive and selective electrochemical sensors for methotrexate (MTX). For this purpose, we have used a simple approach involving the polyaniline (PANI) as a sacrificing template for the growth of uniform and ultra-small Co3O4 nanoparticles by hydrothermal method. The structure, shape, composition and phase purity were studied by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) and Fourier transform Infrared (FTIR) techniques. The average size of Co3O4 nanoparticles was below 50 nm. The cubic crystallography is confirmed for the Co3O4 nanoparticles. The electrochemical properties of PANI assisted Co3O4 nanoparticles for MTX drug was evaluated by cyclic voltammetry (CV) and linear sweep voltammetry (LSV) in Britton–Robinson buffer (BRB) of pH 3.5. The PANI assisted Co3O4 nanoparticles were found highly sensitive for the MTX drug and exhibited a linear range from 5-75µM of MTX and limit of detection for the modified electrode was estimated 1.98µM. The proposed electrochemical sensor is low cost, simple, highly sensitive and selective towards MTX detection. The synthetic methodology using the conducting polymer as a sacrificing template for the growth of controlled and ultra-small Co3O4 nanoparticles can be utilized for the wide range of electrochemical applications.

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“…Using more than one electrical component for predicting analyte concentration could bring advantages inherent to the multivariate calibration approach such as (i) possibility of providing extra (and often complementary) information to the calibration model for each element added, once different elements may be influenced differently by experimental noise or interferents; (ii) possibility of simultaneous analysis of multiple analytes; and (iii) possibility of detecting the presence of interferents (first‐order advantage) 18 . The use of multivariate calibration applied to EIS data for the development of analytical methods is scarce and is mainly based on latent structures methods such as partial least squares (PLS) and principal component regression (PCR) on EIS full spectrum represented as real or complex numbers 19–22 . PCR and PLS show that they can produce impedimetric methods with good predictive abilities and the advantages associated to multivariate calibration, including calibration sets with a relatively small number of standards (or much smaller than the number of spectral variables).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of multiple linear regression applied to impedimetric sensing

Rodrigues

Fragoso

Lemos

2020

Journal of Chemometrics

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In this work, the application of multiple linear regression (MLR) on the development of electroanalytical methods based on electrochemical impedance spectroscopy (EIS) data was investigated. This approach was proposed considering that more than one element of the equivalent electrical circuit fitted to impedance spectra could keep linear relationship with the analyte concentration and be used in a multivariate calibration model, rather than using only one element of the circuit in a univariate regression, the benchmark procedure in the context of impedimetric (bio)sensing. First, MLR was evaluated in the individual determination of the redox probe ferrocyanide in aqueous solutions, followed by the determination of catechol and hydroquinone in tap water samples. MLR produced better predictions than univariate regression when more complex electrochemical systems (catechol and hydroquinone) and more complex samples (tap water) were analyzed. The determinations of hydroquinone and catechol in the direct analysis of spiked tap water samples were successful, with apparent recoveries ranging from 94% to 136%.

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Determination of methotrexate in spiked human blood serum using multi-frequency electrochemical immittance spectroscopy and multivariate data analysis

Cited by 20 publications

References 37 publications

Facile Electrochemical Determination of Methotrexate (MTX) Using Glassy Carbon Electrode-Modified with Electronically Disordered NiO Nanostructures

Facile Electrochemical Determination of Methotrexate (MTX) Using Glassy Carbon Electrode-Modified with Electronically Disordered NiO Nanostructures

Polyaniline as a Sacrificing Template for the Synthesis of Ultra-small Co3O4 Nanoparticles for the Sensitive and Selective Detection of Methotrexate (MTX)

Evaluation of multiple linear regression applied to impedimetric sensing

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