Determination of minimum biofilm eradication concentrations of orbifloxacin for canine bacterial uropathogens over different treatment periods

Shimizu, Takefumi; Harada, Kazuki

doi:10.1111/1348-0421.12461

Cited by 8 publications

(3 citation statements)

References 28 publications

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“…Interestingly, for trimethoprim, similar findings were made when canine uropathogens were tested against TMP-SMX. 30 This could be because of differences in antibiotic permeability in biofilm form, quorum sensing mechanisms, and cell growth heterogeneity. Likewise, pharmacokinetic/pharmacodynamic factors of host and bacteria may also play a role in treatment outcome.…”

Section: Discussionmentioning

confidence: 99%

In-vitro Investigation of Antibiotics Efficacy Against Uropathogenic Escherichia coli Biofilms and Antibiotic Induced Biofilm Formation at Sub-Minimum Inhibitory Concentration of Ciprofloxacin

Rafaque¹,

Abid²,

Liaqat³

et al. 2020

IDR

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Background: Community-acquired urinary tract infections are associated with significant morbidity, and uropathogenic Escherichia coli (UPEC) alone causes 90% of urinary tract infections. This bacterium retains a diverse armament of virulence factors including fimbria, hemolysins, and siderophores production. In a post invasion scenario, formation of intracellular communities mimic biofilm-like characteristics and are linked to recurrent urinary tract infections. We investigated the effects of different frontline antibiotics on the formation, inhibition, and eradication of biofilms of virulent UPEC strains. Materials and Methods: A total of 155 UPEC strains were scrutinized for various virulence factors including gelatinase, cell surface hydrophobicity, hemagglutination, and serum bactericidal activity. Biofilm formation was confirmed by three different methods: Congo red agar, test tube, and tissue culture plate method. Biofilm inhibition and eradication assays were performed according to the standard protocols. Topographical analysis of biofilms was done by scanning electronic microscopy (SEM). Results: Out of 155 strains, 113 (73%) were strong biofilm formesr, while 37 (24%) produced biofilms at moderate level. Significant differences were observed between MICs of planktonic cells (MIC-p) and MICs of UPEC biofilms (MIC-b). Among tested frontline antibiotics, levofloxacin successfully inhibited biofilms at a concentration of 32 µg/mL, while trimethoprim eradicated biofilms at higher concentrations (512-1024 µg/mL). Ciprofloxacin treatment at sub-MIC level significantly enhanced biofilm formation (P<0.05). Conclusion: The majority of UPEC strains are strong biofilm formers and show higher tolerance towards frontline antibiotics in biofilm form. We observed significant inhibitory effects of levofloxacin (32 µg/mL) on UPEC biofilms, while treatment with sub-minimal concentrations of ciprofloxacin significantly enhanced biofilm formation. Out of all tested antibiotics, trimethoprim (512-1024 µg/mL) eradicated UPEC biofilms.

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Section: Discussionmentioning

confidence: 99%

In-vitro Investigation of Antibiotics Efficacy Against Uropathogenic Escherichia coli Biofilms and Antibiotic Induced Biofilm Formation at Sub-Minimum Inhibitory Concentration of Ciprofloxacin

Rafaque¹,

Abid²,

Liaqat³

et al. 2020

IDR

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“…The concept of biofilm formation in veterinary medicine has been understood for some time and its presence when using veterinary orthopaedic implants and in the urinary tract of small animals is well known and studied. 8,9 Donlan 10 defines the concept of biofilm as an 'assemblage of surfaceassociated microbial cells that is enclosed in an extracellular polymeric substance matrix'. These microbial cells form strong attachments to the surface making them more resistant to antimicrobial therapy, and in some cases the antibiotic concentration necessary to kill these microbials is a thousand times that required to kill planktonic bacteria.…”

Section: Biofilmmentioning

confidence: 99%

Chronic axillary wounds in cats: what do we know and how should we manage them?

Paulino¹,

Williams²

2023

Journal of Feline Medicine and Surgery

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Practical relevance: Axillary wounds most often occur in outdoor cats that wear a collar, typically after having been missing. These wounds are commonly chronic and indolent in nature, and although there is so far no consensus on an explanation for this, it is likely that there are several factors involved. Clinical challenges: Axillary wounds are often difficult to manage due to the frequent presence of infection, their histopathological characteristics and their location, where there is excessive tension and movement of the axillary tissues. Initial surgical treatment has a high reported incidence of failure and complications in the literature, with wound breakdown reported most commonly. Giving due consideration to the difficulties of managing these wounds, however, will help practitioners to decrease the occurrence of complications and the need for multiple procedures, and therefore improve the outcome. Equipment: Initial approach and surgical management can be achieved using standard medical equipment and surgical kit available to general practitioners. Evidence base: This review discusses the surgical techniques reported in the literature to have successfully treated chronic axillary wounds and recommendations are also provided based on the authors’ clinical experience.

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“…Em comparação com medicamentos de primeira geração, esses agentes têm aplicações clínicas mais amplas no tratamento de infecções complicadas do trato urinário, pielonefrite, pneumonias, infecções de pele e tecidos moles, infecções por clamídia, prostatite, osteomielite, febres entéricas, cólera e salmonelose, infecções causadas por Pseudomonas aeruginosa, exacerbações agudas de bronquite crônica e pneumonia nosocomial (Eshar, et al, 2018;Kukanich, et al, 2016). Expressam um equilibrado espectro de atividade, com eficácia aumentada contra Streptococcus pneumoniae e menor para P. aeruginosa (Shimizu & Harada, 2017).…”

Section: Quinolonas De Segunda Geraçãounclassified

Uso das fluoroquinolonas em cães e gatos domésticos

Santos

Mariotto

Massitel

et al. 2021

RSD

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As fluoroquinolonas são drogas sintéticas que evoluíram ao longo do tempo, sendo formadas por um grupo de antibióticos desenvolvido a partir do ácido nalidíxico, contendo assim, quatro gerações com diferenças estruturais que alteram o espectro de atividade. As fluroquinolonas são utilizadas em todo o mundo no tratamento de infecções de origem bacteriana. Este estudo teve como objetivo reunir informações sobre o uso das fluoroquinolonas em cães e gatos domésticos. Para a realização desta revisão bibliográfica, foram coletados os dados do período de 2006 a 2019 na literatura disponível no Portal da Capes, no Google Acadêmico e em livros, utilizando-se os seguintes descritores: fluroquinolones in dogs e fluroquinolones in cats, também sendo empregadas estas palavras em português. A partir desta busca pode-se verificar que as fluroquinolonas são indicadas em terapias humanas e veterinárias, e possuem, atualmente, atividade antimicrobiana bastante satisfatória, boa disponibilidade através da via oral, boa difusão para os tecidos, meia-vida prolongada e toxicidade significativamente reduzida. A resistência a este antibiótico pode ser atribuída a fatores como: uso indiscriminado e abusivo, facilidade de acesso, uso errôneo em tratamentos não-infecciosos, entre outros fatores. São indicadas principalmente para cães e gatos com infecções bacterianas de organismos Gram-negativos, Gram-positivos e bactérias anaeróbias. Conclui-se que os antimicrobianos do grupo das quinolonas evoluíram ao longo do tempo, com a intenção de criar antimicrobianos eficazes, de ampla ação, mínimos efeitos adversos e preço acessível.

show abstract

Determination of minimum biofilm eradication concentrations of orbifloxacin for canine bacterial uropathogens over different treatment periods

Cited by 8 publications

References 28 publications

<p>In-vitro Investigation of Antibiotics Efficacy Against Uropathogenic <em>Escherichia coli</em> Biofilms and Antibiotic Induced Biofilm Formation at Sub-Minimum Inhibitory Concentration of Ciprofloxacin</p>

<p>In-vitro Investigation of Antibiotics Efficacy Against Uropathogenic <em>Escherichia coli</em> Biofilms and Antibiotic Induced Biofilm Formation at Sub-Minimum Inhibitory Concentration of Ciprofloxacin</p>

Chronic axillary wounds in cats: what do we know and how should we manage them?

Uso das fluoroquinolonas em cães e gatos domésticos

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