Determination of miRNA derived from exosomes of prostate cancer via toehold-aided cyclic amplification combined with HRP enzyme catalysis and magnetic nanoparticles

Wang, Qinjun; Liu, Jingjian; Zeng, Jiantao; Yang, Zhiming; Ran, Fengying; Wu, Lun; Yang, Ge; Mei, Quanxi; Wang, Xisheng; Chen, Qinhua

doi:10.1016/j.ab.2021.114336

Cited by 16 publications

(5 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Dysregulation of exosome-derived miRNAs has been documented in the progression of prostate cancer, contributing to the reprogramming of target cells by influencing gene expression [ 156 ]. miR-423-5p has been found to play a crucial role in the resistance of prostate cancer cells to chemotherapy.…”

Section: Exosomal Micrornas In Cancer Apoptosismentioning

confidence: 99%

Exosomal microRNAs in regulation of tumor cells resistance to apoptosis

Salehi,

Kamali,

Arab

et al. 2024

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

Section: Exosomal Micrornas In Cancer Apoptosismentioning

confidence: 99%

Exosomal microRNAs in regulation of tumor cells resistance to apoptosis

Salehi,

Kamali,

Arab

et al. 2024

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

“…Techniques for exosomal protein analysis include enzyme linked immunosorbent assay (ELISA), protein blotting and mass spectrometry ( Lee et al, 2021 ; Xu et al, 2021 ). Nucleic acid analysis techniques include second-generation sequencing, gene chips, and polymerase chain reaction (PCR) ( Yu et al, 2021b ; Wang et al, 2021c ; Zhang et al, 2021c ). These analytical techniques have greatly facilitated the research process of exosomes, but there are some limitations: ELISA requires a large number of highly concentrated samples and cannot achieve rapid analysis of complex samples.…”

Section: Detection Of Exosomesmentioning

confidence: 99%

Advances in the Application of Exosomes Identification Using Surface-Enhanced Raman Spectroscopy for the Early Detection of Cancers

Yang

Jia

2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Exosomes are small nanoscale vesicles with a double-layered lipid membrane structure secreted by cells, and almost all types of cells can secrete exosomes. Exosomes carry a variety of biologically active contents such as nucleic acids and proteins, and play an important role not only in intercellular information exchange and signal transduction, but also in various pathophysiological processes in the human body. Surface-enhanced Raman Spectroscopy (SERS) uses light to interact with nanostructured materials such as gold and silver to produce a strong surface plasmon resonance effect, which can significantly enhance the Raman signal of molecules adsorbed on the surface of nanostructures to obtain a rich fingerprint of the sample itself or Raman probe molecules with ultra-sensitivity. The unique advantages of SERS, such as non-invasive and high sensitivity, good selectivity, fast analysis speed, and low water interference, make it a promising technology for life science and clinical testing applications. In this paper, we briefly introduce exosomes and the current main detection methods. We also describe the basic principles of SERS and the progress of the application of unlabeled and labeled SERS in exosome detection. This paper also summarizes the value of SERS-based exosome assays for early tumor diagnosis.

show abstract

“…Moreover, the additional use of prostate-specific membrane antigen (PSMA) aptamer to detect and capture PSMA-positive sEVs from urine samples provided good diagnostic efficiency for PCa ( Li P. et al, 2019 ). Another distinct approach for sEVs carried miRNA was proposed by Wang et al (2021 ). In their study, a biosensor based on magnetic nanoparticles for immobilization of sEV-isolated miRNA and detection with toehold-aided cyclic amplification combined with horseradish peroxidase catalysis.…”

Section: Challenges Of Sevs As Biomarkers For Utcmentioning

confidence: 99%

Small extracellular vesicles as a multicomponent biomarker platform in urinary tract carcinomas

Szeliski

Drewa

Pokrywczyńska

2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

Extracellular vesicles are a large group of nano-sized vesicles released by all cells. The variety of possible cargo (mRNAs, miRNAs, lncRNAs, proteins, and lipids) and the presence of surface proteins, signaling molecules, and receptor ligands make them a rich source of biomarkers for malignancy diagnosis. One of the groups gathering the most interest in cancer diagnostic applications is small extracellular vesicles (sEVs), with ≤200 nm diameter, mainly composed of exosomes. Many studies were conducted recently, evaluating the diagnostic potential of sEVs in urinary tract carcinomas (UTCs), discovering and clinically evaluating various classes of biomarkers. The amount of research concerning different types of UTCs understandably reflects their incidence. sEV cargos getting the most interest are non-coding RNAs (miRNA and lncRNA). However, implementation of other approaches such as metabolomic and proteomic analysis is also evaluated. The results of many studies indicate that sEVs have an essential role in the cancer process and possess many possible diagnostic and prognostic applications for UTC. The relative ease of obtaining biofluids rich in sEVs (urine and blood) confirms that sEVs are essential for UTC detection in the liquid biopsy approach. A noticeable rise in research quality is observed as more researchers are aware of the research standardization necessity, which is essential for considering the clinical application of their findings.

show abstract

Determination of miRNA derived from exosomes of prostate cancer via toehold-aided cyclic amplification combined with HRP enzyme catalysis and magnetic nanoparticles

Cited by 16 publications

References 37 publications

Exosomal microRNAs in regulation of tumor cells resistance to apoptosis

Exosomal microRNAs in regulation of tumor cells resistance to apoptosis

Advances in the Application of Exosomes Identification Using Surface-Enhanced Raman Spectroscopy for the Early Detection of Cancers

Small extracellular vesicles as a multicomponent biomarker platform in urinary tract carcinomas

Contact Info

Product

Resources

About