Determination of MMP‐2 and ‐9 activities in synovial fluid of horses with osteoarthritic and arthritic joint diseases using gelatin zymography and immunocapture activity assays

Fietz, S.; Einspanier, Ralf; Höppner, S.; Hertsch, B; Bondzio, Angelika

doi:10.2746/042516408x251921

Cited by 28 publications

(21 citation statements)

References 23 publications

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“…These observations suggest enhanced expression and activation of MMP-9 in septic knee arthritis compared with the aseptic, which is consistent with previous studies [2,4,7,20]. These observations suggest enhanced expression and activation of MMP-9 in septic knee arthritis compared with the aseptic, which is consistent with previous studies [2,4,7,20].…”

Section: Discussionsupporting

confidence: 92%

“…Arican et al found increased concentrations of MMP-9 activity in the synovial fluid of cows with septic arthritis in comparison with fluids from normal joints, using gelatin zymography and ELISA [2]. Two more animal studies confirmed the above data in horses and calves [7,9]. Two more animal studies confirmed the above data in horses and calves [7,9].…”

Section: Discussionmentioning

confidence: 79%

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Expression levels of matrix metalloproteinase (MMP)-9 and its specific inhibitor TIMP-1, in septic and aseptic arthritis of the knee

Fotopoulos

Τzinia

Tzurbakis

et al. 2011

Knee Surg Sports Traumatol Arthrosc

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 79%

Expression levels of matrix metalloproteinase (MMP)-9 and its specific inhibitor TIMP-1, in septic and aseptic arthritis of the knee

Fotopoulos

Τzinia

Tzurbakis

et al. 2011

Knee Surg Sports Traumatol Arthrosc

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“…Minocycline was tested at ranges from 0 to 4000 μg/mL (8.1 × 10 −6 M). Exhibiting close homology to equine MMPs, human‐recombinant MMP‐2 and MMP‐9 (rhMMP‐2 and ‐9, Calbiotech, Spring Valley, CA) was each used at 10 μg/mL . A stock solution of pure minocycline (Sigma‐Aldrich Fine Chemicals, St Louis, MO) was prepared in PBS.…”

Section: Methodsmentioning

confidence: 68%

The presence of minocycline in the tear film of normal horses following oral administration and its anticollagenase activity

Monk

Jeong

Gibson

et al. 2017

Veterinary Ophthalmology

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Purpose Tetracyclines have activity against matrix metalloproteinases (MMP). Oral medications with effects on the ocular surface are of interest in patients where repeated topical dosing is limited. The aim of this study was to characterize the concentration of minocycline in the tears of normal horses after oral administration and to determine if this level directly inhibits MMP activity. Methods Five healthy adult ponies were administered oral minocycline (Wedgewood Pharmacy; Swedesboro, NJ) at 4 mg/kg every 12 h for 5 days. Tears were collected at T = 2, 26, 50, 56, 74, 80, and 98 h. Tear minocycline concentrations were analyzed using high performance liquid chromatography. The inhibition of recombinant human MMP-2 and MMP-9 by minocycline was investigated using fluorescence resonance energy transfer.Results Minocycline was present in the tears of each pony at every measurement but with interpony variability. A mean concentration of 11.8 lg/mL was present 2 h after administration of the first dose. Minocycline did not directly inhibit MMP-2 or MMP-9 function at a concentration achieved in the pony tear film.Conclusions Minocycline was present in the tears of all ponies at each sampling point following oral administration. One pony of the five had consistently lower levels of minocycline secretion (P ≤ 0.05). The concentration secreted in the tears did not directly inhibit MMP-2 or MMP-9 when tested in vitro. The inconsistencies in the tear concentration and the inhibition activity suggest topical application may be necessary to attain direct inhibition of MMP with minocycline.

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“…It was decreased when the affected horses were successfully treated [32]. It has been reported that the potent stimulators of cartilage degradation such as IL-1 β and TNF- α upregulated MMP-2 gene expression by selectively inducing mitogen-activated protein kinase (MAPK) signaling pathway via MKK3/6-p38, leading to phosphorylation of the activating transcription factor 2 (AP-2) which consequently mediated transcriptional processing of MMP-2 gene [33, 34].…”

Section: Discussionmentioning

confidence: 99%

Andrographolide Exerts Chondroprotective Activity in Equine Cartilage Explant and Suppresses Interleukin-1β-Induced MMP-2 Expression in Equine Chondrocyte Culture

Tangyuenyong

Viriyakhasem

Peansukmanee

et al. 2014

International Scholarly Research Notices

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Cartilage erosion in degenerative joint diseases leads to lameness in affected horses. It has been reported that andrographolide from Andrographis paniculata inhibited cartilage matrix-degrading enzymes. This study aimed to explore whether this compound protects equine cartilage degradation in the explant culture model and to determine its effect on matrix metalloproteinase-2 (MMP-2) expression, a matrix-degrading enzyme, in equine chondrocyte culture. Equine articular cartilage explant culture was induced by 25 ng/mL interleukin-1β, a key inducer of cartilage degeneration, in cultures with or without andrographolide ranging from 10 to 50 μM. After 3–21 days, they were analyzed for the markers of cartilage degradation. It was found that interleukin-1β increased the release of sulfated glycosaminoglycans and hyaluronan from the explants into the culture media consistently with the decrease in uronic acid and collagen content in the cartilage explants. These catabolic effects were inhibited when cotreated with interleukin-1β and andrographolide. In primary equine chondrocytes, andrographolide suppressed interleukin-1β-induced MMP-2 mRNA expression and MMP-2 activity in the culture medium. These results confirmed the in vitro potent chondroprotective activities of this compound which were performed in cartilage explants and on a cellular level. These may indicate the application of andrographolide for therapeutic use in equine degenerative joint diseases.

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Determination of MMP‐2 and ‐9 activities in synovial fluid of horses with osteoarthritic and arthritic joint diseases using gelatin zymography and immunocapture activity assays

Abstract: These findings encourage the potential use of MMP-2 and -9 as additional aids to clinical investigation. Further work is required to validate the clinical significance of MMP activities in the progress of different joint diseases in horses.

Cited by 28 publications

References 23 publications

Expression levels of matrix metalloproteinase (MMP)-9 and its specific inhibitor TIMP-1, in septic and aseptic arthritis of the knee

Expression levels of matrix metalloproteinase (MMP)-9 and its specific inhibitor TIMP-1, in septic and aseptic arthritis of the knee

The presence of minocycline in the tear film of normal horses following oral administration and its anticollagenase activity

Andrographolide Exerts Chondroprotective Activity in Equine Cartilage Explant and Suppresses Interleukin-1β-Induced MMP-2 Expression in Equine Chondrocyte Culture

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