Determination of modification degree in BDDE-modified hyaluronic acid hydrogel by SEC/MS

Yang, Biao; Guo, Xia; Zang, Hengchang; Jian-jian, Liu

doi:10.1016/j.carbpol.2015.05.050

Cited by 45 publications

(22 citation statements)

References 21 publications

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“…As very few studies in the literature have reported, our data indicated that attention must be paid when ranking the gels in terms of their HA modification parameters that are based only on the BDPE/HA d ratio [3,12,19,31]. In fact, since the bridging BDPE/total BDPE ratio greatly varied from one product to another (Table 1 (D)), the rankings in the HA crosslinking degree (G) can be variously marked or even completely different compared to those for the BDPE/HA ratio (A) or total HA modification level (I).…”

Section: Discussionmentioning

confidence: 88%

“…Conversely, characterization studies of the structural modifications obtained after the reaction of HA with BDDE have been performed to a lower extent [2][3][4][5]9,12,19,20,[29][30][31]. Only some of them have discriminated between the BDPE-bridging and BDPE-pendant moieties.…”

Section: Introductionmentioning

confidence: 99%

“…Only some of them have discriminated between the BDPE-bridging and BDPE-pendant moieties. Furthermore, these analyses were mostly performed for homemade gels, and the acquired chemical data have never been considered in relation to hydrogel rheology [3][4][5]12,19,31].…”

Section: Introductionmentioning

confidence: 99%

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Hyaluronan Hydrogels: Rheology and Stability in Relation to the Type/Level of Biopolymer Chemical Modification

et al. 2022

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BDDE (1,4-butanediol-diglycidylether)-crosslinked hyaluronan (HA) hydrogels are widely used for dermo-aesthetic purposes. The rheology and stability of the gels under physiological conditions greatly affect their clinical indications and outcomes. To date, no studies investigating how these features are related to the chemistry of the polymeric network have been reported. Here, four available HA-BDDE hydrogels were studied to determine how and to what extent their rheology and stability with respect to enzymatic hydrolysis relate to the type and degree of HA structural modification. 1H-/13C-NMR analyses were associated for the quantification of the “true” HA chemical derivatization level, discriminating between HA that was effectively crosslinked by BDDE, and branched HA with BDDE that was anchored on one side. The rheology was measured conventionally and during hydration in a physiological medium. Sensitivity to bovine testicular hyaluronidase was quantified. The correlation between NMR data and gel rheology/stability was evaluated. The study indicated that (1) the gels greatly differed in the amounts of branched, crosslinked, and overall modified HA, with most of the HA being branched; (2) unexpectedly, the conventionally measured rheological properties did not correlate with the chemical data; (3) the gels’ ranking in terms of rheology was greatly affected by hydration; (4) the rheology of the hydrated gels was quantitatively correlated with the amount of crosslinked HA, whereas the correlations with the total HA modification level and with the degree of branched HA were less significant; (5) increasing HA derivatization/crosslinking over 9/3 mol% did not enhance the stability with respect to hyaluronidases. These results broaden our knowledge of these gels and provide valuable information for improving their design and characterization.

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Hyaluronan Hydrogels: Rheology and Stability in Relation to the Type/Level of Biopolymer Chemical Modification

et al. 2022

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“…The reaction consists of the epoxide ring opening to form ether bonds with the HA hydroxyl groups. 29 …”

Section: Modification Of Hamentioning

confidence: 99%

“…The reaction consists of the epoxide ring opening to form ether bonds with the HA hydroxyl groups. 29 HA crosslinking with divinyl sulfone (DVS) [30][31][32] was performed at room temperature in high pH conditions (pH > 8). The advantage of this system is limitation of the degradation of HA in alkaline solutions compared to that observed at high temperature.…”

Section: Chemical Modifications and Crosslinking Of Hamentioning

confidence: 99%

Crosslinking method of hyaluronic-based hydrogel for biomedical applications

2017

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In the field of tissue engineering, there is a need for advancement beyond conventional scaffolds and preformed hydrogels. Injectable hydrogels have gained wider admiration among researchers as they can be used in minimally invasive surgical procedures. Injectable gels completely fill the defect area and have good permeability and hence are promising biomaterials. The technique can be effectively applied to deliver a wide range of bioactive agents, such as drugs, proteins, growth factors, and even living cells. Hyaluronic acid is a promising candidate for the tissue engineering field because of its unique physicochemical and biological properties. Thus, this review provides an overview of various methods of chemical and physical crosslinking using different linkers that have been investigated to develop the mechanical properties, biodegradation, and biocompatibility of hyaluronic acid as an injectable hydrogel in cell scaffolds, drug delivery systems, and wound healing applications.

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Modification and crosslinking strategies for hyaluronic acid‐based hydrogel biomaterials

Luo,

Wang,

et al. 2023

Smart Medicine

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Hyaluronic acid (HA) is an attractive extracellular matrix‐derived polymer. The related HA‐based hydrogels are emerging to be the hotspots in the cutting edge of biomaterials. The continuous sights concentrate on exploring modification methods and crosslinking strategies to promote the advancement of HA‐based hydrogels with enhanced physical/chemical properties and enriched biological performance. Here, the advances on modification methods and crosslinking strategies for fabricating HA‐based hydrogels with diverse capacities are summarized. Firstly, the modification reactions that occur on the active hydroxyl, carboxyl and N‐acetyl groups of HA molecule are discussed. Next, the emphasis is put on various crosslinking strategies including physical crosslinking, covalent crosslinking and dynamic covalent crosslinking. Finally, we provide a general summary and give a critical viewpoint on the remaining challenges and the future development of HA‐based hydrogels. It is hoped that this review can provide new proposals for the specific design of functional hydrogel biomaterials.

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Determination of modification degree in BDDE-modified hyaluronic acid hydrogel by SEC/MS

Cited by 45 publications

References 21 publications

Hyaluronan Hydrogels: Rheology and Stability in Relation to the Type/Level of Biopolymer Chemical Modification

Hyaluronan Hydrogels: Rheology and Stability in Relation to the Type/Level of Biopolymer Chemical Modification

Crosslinking method of hyaluronic-based hydrogel for biomedical applications

Modification and crosslinking strategies for hyaluronic acid‐based hydrogel biomaterials

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