Determination of Mogroside V in Luohanguo Extract for Daily Quality Control Operation Using Relative Molar Sensitivity to Single-Reference Caffeine

Masumoto, Naoko; Ishizuki, Kyoko; Nishizaki, Yuzo; Ohtsuki, Takashi; Kuroe, Miho; Yamazaki, Taichi; Numata, Masahiko; Matsufuji, Hiroshi; Sugimoto, Naotoshi; Sato, Kyoko

doi:10.1248/cpb.c20-00245

Cited by 7 publications

(2 citation statements)

References 10 publications

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“…Modern pharmacological research has identified mogrosides as the main active components of siraitia grosvenorii, with MV being one of the major bioactive components (Xiao et al., 2021), which is constructed by linking mogrol with five glucopyranosides (Figure 1) (Xu et al., 2015; Yan et al., 2018). MV is widely used in various applications such as food additives, anti‐ageing products, anti‐inflammatory agents, and novel drug carriers (Masumoto et al., 2021; Zhang et al., 2022; Zhou et al., 2018). Recent studies have confirmed that MV can improve the quality of porcine oocytes by suppressing inflammation and scavenging free radical ions (Pan et al., 2022; Yan et al., 2021).…”

Section: Introductionmentioning

confidence: 99%

Smart seq2 revealed distinct molecular responses during in vitro porcine oocyte maturation before or after the addition of mogroside V

Nie,

Xiao,

Xiong

et al. 2024

Reprod Domestic Animals

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Oocyte maturation involves both nuclear and cytoplasmic maturation. Mogroside V (MV) has been shown to enhance nuclear maturation, mitochondrial content, and developmental potential of porcine oocyte during in vitro maturation (IVM). However, the impact of MV on cytoplasmic maturation and its underlying mechanisms are not understood. This study aimed to assess the effect of MV on cytoplasmic maturation. Germinal vesicle (GV) oocytes treated with MV exhibited a noticeable increase in cortical granules (CGs) formation. Additionally, MV enhanced the expression of NNAT and improved glucose uptake in mature oocytes. Further insights were gained through Smart‐seq2 analysis of RNA isolated from 100 oocytes. A total of 11,274 and 11,185 transcripts were identified in oocytes treated with and without MV, respectively. Among quantified genes, 438 differentially expressed genes (DEGs) were identified for further analysis. Gene Ontology (GO) enrichment analysis indicated that these DEGs were primarily involved in DNA repair regulation, cellular response to DNA damage, intracellular components, and organelles. Furthermore, the DEGs were significantly enriched in three KEGG pathways: fatty acid synthesis, pyruvate metabolism, and WNT signalling. To validate the results, lipid droplets (LD) and triglyceride (TG) were examined. MV led to an increase in the accumulation of LD and TG production in mature oocytes. These findings suggest that MV enhances cytoplasmic maturation by promoting lipid droplet synthesis. Overall, this study provides valuable insights into the mechanisms through which MV improves oocyte quality during IVM. The results have significant implications for research in livestock reproduction and offer guidance for future studies in this field.

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Section: Introductionmentioning

confidence: 99%

Smart seq2 revealed distinct molecular responses during in vitro porcine oocyte maturation before or after the addition of mogroside V

Nie,

Xiao,

Xiong

et al. 2024

Reprod Domestic Animals

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“…13) Single reference (SR)-LC assay based on relative molar sensitivity (RMS) is a recently applied method for the quantitative determination of natural compounds in various food materials. [14][15][16][17] Each natural analyte could be quantitated according its intensity ratio with SR coefficient on a one-running chromatogram. 18) Compare to conventional LC evaluation, SR-LC assay demonstrates that the calibration analysis of RMS using 1 H-quantitative nuclear magnetic resonance spectroscopy (qNMR) is a simple and reliable quantification that does not require these certified references.…”

Section: Introductionmentioning

confidence: 99%

Study on the Synthesis of Methylated Reference and Their Application in the Quantity of Curcuminoids Using Single Reference Liquid Chromatography Based on Relative Molar Sensitivity

Takahashi

Nishizaki²,

Masumoto³

et al. 2022

CHEMICAL & PHARMACEUTICAL BULLETIN

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We report on the recommendation of the simple and versatility of methylated reference (MR) to improve applications in the single reference (SR)-LC based on relative molar sensitivity (RMS). Three curcuminoids (Curs) such as curcumin, demethoxycurcumin and bisdemethoxycurcumin in turmeric products were determined using authentic standards and methylated curcumin. In addition, high-speed countercurrent chromatography (HSCCC) purification is necessary to separate Curs for indicating the RMS. For HSCCC separation, a biphasic solvent system was used to obtain these fractions, which were then subjected to 1 H quantitative NMR to determine their contents in each test solution. Using these solutions, the RMS of Curs are calculated from slopes ratios of calibration curves (three ranges from 0-100 µmol/L, r 2 > 0.998). The averaged RMS of Curs were 8.92 (relative standard deviation (RSD), 1.17%), 8.97 (2.18%), and 9.61 (0.77%), respectively. Cur concentrations in turmeric products can be determined using RMS, peak area, and MR content added in these samples. This proposed method, which is based on chemical methylation and the SR-LC assay has been successfully applied for the simple and reliable estimation of Curs in turmeric products.

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Quantitative NMR in Quality Control

Liu

2024

Quality Control of Chinese Medicines

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Determination of Mogroside V in Luohanguo Extract for Daily Quality Control Operation Using Relative Molar Sensitivity to Single-Reference Caffeine

Cited by 7 publications

References 10 publications

Smart seq2 revealed distinct molecular responses during in vitro porcine oocyte maturation before or after the addition of mogroside V

Smart seq2 revealed distinct molecular responses during in vitro porcine oocyte maturation before or after the addition of mogroside V

Study on the Synthesis of Methylated Reference and Their Application in the Quantity of Curcuminoids Using Single Reference Liquid Chromatography Based on Relative Molar Sensitivity

Quantitative NMR in Quality Control

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