Determination of molecular species of lecithin from erythrocytes and plasma

Golde, L.M.G. Van; Tomasi, Víctor H.; Deenen, L.L.M. Van

doi:10.1016/0009-3084(67)90035-7

Cited by 68 publications

(27 citation statements)

References 18 publications

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“…Palmitoyloleoyl PC and palmitoyllinoleoyl PC, as well as egg PC and PC from rat liver microsomes (data not shown), are perfectly capable to replace the native erythrocyte PC without any noticeable effect on membrane permeability or stability. This type of mixed acid PC species in which one saturated fatty acid and one unsaturated fatty acid containing one or two double bonds, are present, is the most common PC in the human erythrocyte membrane and comprises up to 60% of the total species composition [3]. It is obvious that this PC offers the most favourable configuration for a stable membrane and that both increasing or decreasing the degree of unsaturation of the PC results in abnormalities.…”

Section: Discussionmentioning

confidence: 99%

“…phospholipid constituents, minor amounts of phosphatidylinositol, phosphatidic acid and lysophospholipids, and furthermore a large quantity of cholesterol [1]. The fatty acid composition of each of the four major phospholipid classes has been clarified, while of PC, phosphatidylethanolamine and sphingomyelin even the fatty acyl species composition is known [2,3]. Finally, the distribution of the different phospholipid classes as well as of their molecular species over 0005-2736/84/$03.00 © 1984 Elsevier Science Publishers B.V.…”

Section: Introductionmentioning

confidence: 99%

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The membrane of intact human erythrocytes tolerates only limited changes in the fatty acid composition of its phosphatidylcholine

Kuypers

Roelofsen

Kamp

et al. 1984

Biochimica et Biophysica Acta (BBA) - Biomembranes

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(1) Using the phosphatidylcholine specific transfer protein from bovine liver, native phosphatidylcholine from intact human erythrocytes was replaced by a variety of different phosphatidylcholine species without altering the original phospholipid and cholesterol content. (2) The replacement of native phospharidylcholine by the disaturated species, 1,2-dipalmitoyl-and 1,2-distearoylphosphatidyicholine, proceeded at a low rate and extensive replacement could only be achieved by repeatedly adding fresh donor vesicles. The replacement by disaturated molecules was accompanied by a gradual increase in osmotic fragility of the cells, finally resulting in hemolysis when 40% of the native PC had been replaced. Up to this lyric concentration, the replacement did not affect the permeability of the membrane for potassium ions. (3) Essentially, all of the PC in the outer monolayer of the membrane could be replaced by 1-palmitoyl-2-oleoyl-and 1-palmitoyl-2-1ino-leoylphosphatidylcholine. These replacements did not alter the osmotic fragility of the cells, nor the K + permeability of the membrane. (4) Increasing the total degree of unsaturation of the phosphatidyicholine species modified the properties of the membrane considerably. Replacement by 1,2-dilinoleoylphosphatidylcholine resulted in a progressive increase in osmotic fragility and hemolysis started to occur after 30% of the native PC had been replaced by this species. K ÷ permeability was found to be slightly increased in this case. Cells became leaky for K ÷ upon the introduction of 1-palmitoyl-2-arachidonoylphosphatidyicholine in the membrane. The increased permeability was also reflected by an apparent increase in the resistance of the cells against osmotic shock. (5) The conclusions to be drawn are that (i) 1-palmitoyl-2-oleoyi-and l-paimitoyi-2-1inoleoylphosphatidyicholine are species which fit most optimally into the erythrocyte membrane; (ii) loss of membrane stability results from an increase in the degree of saturation of phosphatidyicholine (unsaturation index > 0.5) and (iii) the permeability is enhanced by increasing the content of highly unsaturated species (unsaturation index > 1.0).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The membrane of intact human erythrocytes tolerates only limited changes in the fatty acid composition of its phosphatidylcholine

Kuypers

Roelofsen

Kamp

et al. 1984

Biochimica et Biophysica Acta (BBA) - Biomembranes

Self Cite

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“…The lecithins were then degraded with phospholipase A2 and assayed for radioactivity [17]. The details of this procedure have been described previously [19]. The positional distribution of the fatty acids incorporated into the lecithin located at the inside of the membrane is not different from that observed for the total lecithin of the erythrocyte.…”

Section: * Including P H O S P H a T I D Y L I N O S I T O Lmentioning

confidence: 99%

“…It is known [19] that lecithin of the erythrocyte represents a heterogeneous collection of molecules differing with respect to their fatty acylconstituents. In this respect it could be speculated that the various individual molecular species of lecithin could be distributed in a non-random way among the outer and inner layer of the erythrocyte membrane.…”

Section: * Including P H O S P H a T I D Y L I N O S I T O Lmentioning

confidence: 99%

Preferential incorporation of fatty acids at the inside of human erythrocyte membranes

Renooij

Golde

Zwaal

et al. 1974

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Summary1. Phospholipase A2 isolated from Naja naja venom was used as a tool to discriminate between the outer and inner lipid monolayer of the membranes of human erythrocytes.2. Incubation of human erythorcytes with radioactive fatty acids resulted in the formation of labelled lecithin in the membrane. The label was found predominantly in that pool of lecithin which is localized in the inner monolayer of the membrane.3. Lecithins isolated from the total erythrocyte membrane and from the inner monolayer of the membrane possess identical fatty acid patterns and identical positional distributions of their fatty acyl constituents.Circulating red blood cells should be capable of incorporating fatty acids into their phospholipids since variations in the diet can induce alterations in the fatty acid pattern of the phospholipids within a much shorter period than the average lifespan of the erythrocyte [1,2], Two mechanisms have been described to play a major role in the renewal of the phospholipids of the erythrocyte membrane: (1) an exchange of phospholipids between serum lipoproteins and the red cell membrane [3--5] and (2) the incorporation of fatty acids into lysophospholipids which are either formed in the membrane or supplied by the surrounding serum [6,7]. Shohet [8,9] proposed the existence of two compartments of lecithin in the erythrocyte membrane: one of these is thought to be in equilibrium with lecithin from

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“…This additional fractionation aided the identification and quantification of FAME which appeared in overlapping peaks on GLC. The fractions were made visible in ultraviolet light by spraying with rhodamine B [16,40].…”

Section: Separation Of Phospholipids and Fatty Acid Methyl Estersmentioning

confidence: 99%

Fatty Acid Composition of Phospholipids in Erythrocytes of Adults, Normal Newborn Infants, and Neonates with Rh Erythroblastosis

et al. 1972

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Determination of molecular species of lecithin from erythrocytes and plasma

Cited by 68 publications

References 18 publications

The membrane of intact human erythrocytes tolerates only limited changes in the fatty acid composition of its phosphatidylcholine

The membrane of intact human erythrocytes tolerates only limited changes in the fatty acid composition of its phosphatidylcholine

Preferential incorporation of fatty acids at the inside of human erythrocyte membranes

Fatty Acid Composition of Phospholipids in Erythrocytes of Adults, Normal Newborn Infants, and Neonates with Rh Erythroblastosis

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