Determination of mono- and diglycerides by capillary gas chromatography: results of a collaborative study and the standardized method

Abstract

Determination of mono-and diglycerides by capillary gas chromatography: results of a collaborative study and the standardized method Abstract-A method for the determination of mono-and diglycerides by capillary gas chromatography has been elaborated and tested in a collaborative study. The procedure involves conversion of the mono-and diolvcerides into more volatile trimethvlsilvl ether derivatives usino N,N-bis(trimet~visilvl)trifluoroacetamide (BSTFA). and trimethvlchlorosilane (TMCSJ (3:1) in pyridine.• n-Tetradecane was used as internal standard. Capillarv gas chromatographv with split or on-column injection was applied. The samples analysed consisted of two commercial mono-, diglyceride emulsifiers. two mixtures of known comoosition of mono-and diglycerides present in an excess of triglycerides. and of refined sunflower oil spiked with known amounts of mono-and diglvceriaes. Besides mono-and diglycerides other components of fats and oils such as glycerol, fattv acids, sterols etc. mav be converted into the trimethvlsilvl ether derivatives and ~nalysea bv the same GC procedure. For the identificati~n of. the components coupled gas chromatography/mass spectrometry is acvantageous.

Enzymatic synthesis of 1,3-dicaproyglycerol by esterification of glycerol with capric acid in an organic solvent system

“…When those undesirable dicaproyglycerols are esterified, tricaprin (CCC) is generated as product (more information on acyl migration is presented in Section 3.3). The capillary column used differentiates positional isomers, and their identification was possible using the work by Bruschweiler and Dieffenbacher [28], who proposed a method for determining mono-and diglycerides by gas chromatography.…”

In Vitro Digestibility and Quality of an Emulsified Meat Product Formulated With Animal Fat Encapsulated With Pectin

“…Chloroform–lipid lower layer was recovered, placed, in a 50 mL Falcon tube, washed with 4 mL of a water:methanol solution (2:2), and before storing the sample, all air was removed from the tube by flushing with nitrogen (industrial grade; Praxair, Monterrey, NL, Mexico). After that lipid extracts were derivatizated using the method reported by Bruschweiler and Dieffenbacher (). An aliquot of 20 mg of lipid extract was placed inside a 1.5 mL vial with 100 µL pyridine, 200 µL de Bis‐(trimethylsilyl)‐trifluoroacetamide with trimethylchlorosilane (BSTFA+TMCS; Sigma‐Aldrich), and 20 µL of butanietrol (Agilent Technologies, St. Clara, CA, USA) as an internal standard.…”

Food Emulsifiers and Their Applications

“…GLC analysis is accomplished by reaction of a dry sample with chlorotrimethylsilane and hexamethyldisilazane in the presence of pyridine (Nakanishi and Tsuda, 1983;Brueschweiler and Dieffenbacher, 1991;Firestone, 2005ah). GC/MS analysis has also been reported (Lee, 1988).…”