Determination of monoamine neurotransmitters in human urine by carrier-mediated liquid-phase microextraction based on solidification of stripping phase

Jiang, Liwei; Chen, Yibang; Chen, Yejun; Ma, Ming; Tan, Yueming; Tang, Hao; Chen, Bin

doi:10.1016/j.talanta.2015.06.068

Cited by 21 publications

(4 citation statements)

References 35 publications

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“…Currently, solidphase extraction (SPE) is the most frequently employed extraction technique as it offers high selectivity, precision and extraction yields of above 90% [21]. Liquid-phase micro-extraction (LPME) is a relatively newly developed extraction process described to have low solvent consumption [22]. As the extraction of hydrophilic compounds in the organic phase is more difficult, most studies focus on extracting hydrophobic compounds [23].…”

Section: Introductionmentioning

confidence: 99%

“…As the extraction of hydrophilic compounds in the organic phase is more difficult, most studies focus on extracting hydrophobic compounds [23]. To overcome the challenges involved in LPME, Jiang et al [22] developed a novel method to solve the collection of acceptor solutions through the solidification of floating organic drop. Another established sample preparation method for biological samples is sample dilution.…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of a simple, rapid and sensitive LC-MS/MS method for the measurement of urinary neurotransmitters and their metabolites

et al. 2017

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Neurotransmitters play crucial roles in physiological functions and their imbalances have demonstrated association in the pathology of several diseases. The measurement of neurotransmitters possesses a great potential as a significant clinical tool. This study presents the development and validation of an LC-MS/MS method for simultaneous quantification of multi-class neurotransmitters associated with dopamine, tryptophan and glutamate-γ-aminobutyric acid pathways. A total of ten neurotransmitters and their metabolites (dopamine, epinephrine, metanephrine, tryptophan, serotonin, kynurenic acid, kynurenine, anthranilic acid, GABA, glutamic acid) were determined based on a simple and rapid 'dilute and shoot' method using minimal urine volume. The chromatographic separation was achieved using a Poroshell 120 Bonus-RP LC Column in combination with a gradient elution within an 8.5-min time frame. The method exhibited good sensitivity as the limits of quantification ranged between 0.025 and 0.075 μg/mL with acceptable matrix effects (< ± 14.5%), no carryover and good linearity (r > 0.98). The accuracy and precision for all analytes were within tolerances, at < ± 9.9% mean relative error (MRE) and < 8.6% relative standard deviation (RSD), respectively. The method was successfully applied in measuring the neurotransmitter concentrations in urine of healthy donors. Furthermore, the undertaken stability experiments indicated that acidified urine specimens allowed the analytes to be stable for prolonged durations in comparison to those untreated. The study also reveals the performance of the method is unaffected by the absence of expensive deuterated reference standards under the experimental conditions employed which further simplifies the analytical procedures and provides a significant cost saving for running the assay. Graphical abstract The quantification of multi-class neurotransitters associated with the dopamine, tryptophan and GABA-glutamate pathways using a simple 'dilute and shoot' LC-MS/MS method.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and validation of a simple, rapid and sensitive LC-MS/MS method for the measurement of urinary neurotransmitters and their metabolites

et al. 2017

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“…The traditional methods for determination of DA and 5‐HT are high‐performance liquid chromatography–electrochemical detection (HPLC–ECD) (Jiang et al, ). However, the analysis process is too long because only isocratic elution is used in the separation process.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of N‐ethylpentylone, dopamine, 5‐hydroxytryptamine and their metabolites in rat brain microdialysis by liquid chromatography tandem mass spectrometry

Lin

Wang

et al. 2019

Biomedical Chromatography

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N-Ethylpentylone (NEP) is a popular synthetic cathinone abused worldwide. To obtain more information about its pharmacokinetics and pharmacodynamics, a rapid, simple and sensitive liquid chromatography-tandem mass spectrometry method was developed for the determination of NEP, two important neurotransmitters, dopamine and serotonin, and their metabolites, including 3,4-dihydroxyphenylacetic acid, 3methoxytyramine and 5-hydroxyindole-3-acetic acid, in rat brain microdialysate.The analytes were separated on a Phnomenex Polar C 18 column, with a mobile phase of 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B) under gradient elution to shorten the total chromatographic run time. A triple quadruple mass spectrometer coupled with an electrospray ionization source in both positive and negative ion mode was used to detect the analytes. This method showed excellent accuracy (87.4-113.5%) and precision (relative standard deviation <15%) at three quality control levels. The limits of detection were 0.2 ng/mL for NEP and 0.2-50 nM for the others and good linearity was obtained. This study pioneered a method to integrate exogenous drugs and endogenous neurotransmitters as the drugs act on the same determination system, which means that this innovation can provide support for further study of the addictive effects of NEP or other synthetic cathinones on extracellular levels of dopamine and 5-hydroxytryptamine.

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“…Generally, a sample preparation method is required prior to instrumental analysis. A liquid-phase micro-extraction is a relatively newly developed extraction process consuming low solvent consumption [6].…”

Section: Introductionmentioning

confidence: 99%

Transportation of Different Therapeutic Classes of Pharmaceuticals to the Surface Water, Sewage Treatment Plant, and Hospital Samples, Malaysia

Al-Qaim

Mussa

Yuzir

et al. 2018

Water

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All pharmaceuticals are separated chromatographically using the liquid chromatographytime of flight/mass spectrometry (LC-ToF/MS) on a 5 µm, 2.1 mm × 250 mm, C18 column at 0.3 mL/min. The recovery is investigated at two spiking levels, 10 and 1 ng/mL; the mean recovery is higher than 77, 84, and 93% in sewage treatment plants (STP) influent, STP effluent, and surface water, respectively. The limit of quantification (LOQ) averages 29, 16, 7, and 2 ng/L in STP influent, STP effluent, surface water, and drinking water, respectively. The matrix effect is also evaluated in STP influent and effluent. It is observed that sulfamethoxazole, prednisolone, ketoprofen, and glibenclamide are highly impacted compared to other compounds, −99, −110, 77, and 91%, respectively. The results show that six out of nine pharmaceuticals, namely atenolol, acetaminophen, theophylline, caffeine, metoprolol, and sulfamethoxazole are detected in STP influent, STP effluent, and surface water. However, the means of concentration are 561, 3305, 1805, 3900, 78, and 308 ng/L for atenolol, acetaminophen, theophylline, caffeine, metoprolol, and sulfamethoxazole, respectively, in STP influent. Caffeine and acetaminophen are detected with the highest concentration, reaching up to 8700 and 4919 ng/L, respectively, in STP influent.

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Determination of monoamine neurotransmitters in human urine by carrier-mediated liquid-phase microextraction based on solidification of stripping phase

Cited by 21 publications

References 35 publications

Development and validation of a simple, rapid and sensitive LC-MS/MS method for the measurement of urinary neurotransmitters and their metabolites

Development and validation of a simple, rapid and sensitive LC-MS/MS method for the measurement of urinary neurotransmitters and their metabolites

Simultaneous determination of N‐ethylpentylone, dopamine, 5‐hydroxytryptamine and their metabolites in rat brain microdialysis by liquid chromatography tandem mass spectrometry

Transportation of Different Therapeutic Classes of Pharmaceuticals to the Surface Water, Sewage Treatment Plant, and Hospital Samples, Malaysia

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