Determination of MTBE, TBA and BTEX in Soil by Headspace Gas Chromatography-Mass Spectrometry

Shin, Ho‐Sang

doi:10.5012/bkcs.2012.33.5.1693

Cited by 13 publications

(7 citation statements)

References 19 publications

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“…Hydrocarbon analysis was performed on a Varian 450-GC instrument attached to a 240-IT mass spectrometer equipped with a programmed temperature vaporization/split-splitless injector and a CTCGCpal autosampler, as previously described (41). A static headspace gas chromatography mass spectrometer (Varian 450GC 240MS with a CTC CombiPal autosampler) was used for the determination of BTEX in contaminated sediments (44). Briefly, duplicate samples of 2 g of defrosted sediment were placed into 10-ml vials.…”

Section: Methodsmentioning

confidence: 99%

Diversity of Benzylsuccinate Synthase-Like ( bssA ) Genes in Hydrocarbon-Polluted Marine Sediments Suggests Substrate-Dependent Clustering

Acosta-González

Rosselló‐Móra

Marqués

2013

Appl Environ Microbiol

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bThe potential of hydrocarbon biodegradation in marine sediments was determined through the detection of a functional biomarker, the bssA gene, coding for benzylsuccinate synthase, the key enzyme of anaerobic toluene degradation. Eight bssA clone libraries (409 sequences) were constructed from polluted sediments affected by the Prestige oil spill in the Atlantic Islands National Park and from hydrocarbon-amended sediment microcosms in Mallorca. The amplified products and database-derived bssA-like sequences grouped into four major clusters, as determined by phylogenetic reconstruction, principal coordinate analysis (PCoA), and a subfamily prediction tool. In addition to the classical bssA sequences that were targeted, we were able to detect sequences homologous to the naphthylmethylsuccinate synthase gene (nmsA) and the alkylsuccinate synthase gene (assA), the bssA homologues for anaerobic 2-methylnaphthalene and alkane degradation, respectively. The detection of bssA-like variants was determined by the persistence and level of pollution in the marine samples. The observed level of gene diversity was lower in the Mallorca sediments, which were dominated by assA-like sequences. In contrast, the Atlantic Islands samples, which were highly contaminated with methylnaphthalene-rich crude oil, showed a high proportion of nmsA-like sequences. Some of the detected genes were phylogenetically related to Deltaproteobacteria communities, previously described as the predominant hydrocarbon degraders at these sites. Differences between all detected bssA-like genes described to date indicate separation between marine and terrestrial sequences and further subgrouping according to taxonomic affiliation. Global analysis suggested that bssA homologues appeared to cluster according to substrate specificity. We observed undetected divergent gene lineages of bssA homologues, which evidence the existence of new degrader groups in these environments.

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Section: Methodsmentioning

confidence: 99%

Diversity of Benzylsuccinate Synthase-Like ( bssA ) Genes in Hydrocarbon-Polluted Marine Sediments Suggests Substrate-Dependent Clustering

Acosta-González

Rosselló‐Móra

Marqués

2013

Appl Environ Microbiol

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“…The vials were placed in a heating block for 45 min at 50°C. Extraction of volatile compounds was done using a 100 μm Polydimethylsiloxane fibre in a SPME manual holder [8,11]. Direct injection in HS mode was performed in 280 o C in a PTV injector.…”

Section: Preparation Of Samples For Btex Analyzesmentioning

confidence: 99%

“…1μl extract in Dichloromethane (extracting solvent) were injected for PAH analyses. Quantification of BTEX and PAH was based on external standards [6,11].…”

Section: Apparatus and Chromatographymentioning

confidence: 99%

Determination of some organic pollutants in waters rivers of Tirana city

Nuro¹,

Marku²,

Murtaj³

et al. 2017

Zas Mat

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This study presented data about concentrations of some organic pollutants in two main rivers of Tirana city: Lana River that crosses through the city and Tirana River that lies in northeast of the city. Twenty stations were analyzed in this study. Sampling of water was realized in July 2016. These rivers present important aquatic areas of Tirana region in Albania. For both water river samples were analyzed organochlorine pesticides (OCPs), their degradation products, polychlorinated biphenyls (PCBs), BTEX (benzene, toluene, ethylbenzene and o-, m-, p-xylenes) and polyromantic hydrocarbons (PAH). For extraction of OCPs and PCBs by water samples were used liquid-liquid (LLE) extraction and n-Hexane as extracting solvent. After clean-up and concentration procedures for all samples were performed gas chromatographic analyzes using Rtx-5 capillary column (30 m x 0.33 mm x 0.25 um) and electron capture detector (GC/ECD). Headspace solid phase micro extraction (HS-SPME) technique was used to trace BTEX in water samples. For isolation of PAH, LLE extraction assisted with Dichloromethane as extraction solvent were used. The extracts were concentrated and then were injected in gas chromatograph. The analysis of BTEX and PAH in water samples was performed by gas chromatography technique using flame ionization detector (GC/FID). Injections of BTEX were done directly by using Head-Space mode of Polydimethyl Siloxane fiber. VF-1ms capillary column (30 m x 0.33 mm x 0.25 um) was used for separation of BTEX and PAH compounds. The higher concentrations of organochlorine pesticides and PCBs were found for water samples of Tirana River because of their previous use, in the agricultural areas near the basin of this river. The higher concentrations were for volatile PCBs because of their atmospheric deposition in Albanian territory. Relatively high concentrations of BTEX and more volatile PAH compounds were detected in water samples of Lana River. The presence of volatile organic pollutants could be mostly of automobilist transport near the stations, discharging of wastes from some gas stations or some mechanical industries neat the river.

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“…The vials were placed in a heating block for 45 min at 50°C. Extraction of volatile compounds was done using a 100 μm Polydimethylsiloxane fibre in a SPME manual holder [9,10]. Direct injection in HS mode was performed in 280oC in a PTV injector.…”

Section: Preparation Of Samples For Btex Analyzesmentioning

confidence: 99%

“…1 ul extract in Dichloromethane (extracting solvent) were injected for PAH analyses. Quantification of BTEX and PAH was based on external standards [6,9,10].…”

Section: Apparatus and Chromatographymentioning

confidence: 99%

Analysis of organic pollutants in water samples of White Drin, Black Drin and Fierza Reservoir

Nuro

Marku

Murtaj

2017

J. eng. process. manag.

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This study presented data about concentrations of some organic pollutants in Albanian part of Black Drin (BD), White Drin (WD) and Fierza Reservoir (FR). Thirteen stations were analyzed in this study. Sampling of water was realized in May 2017. Drin River presents important aquatic area in Albania. For all water samples were analyzed organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), benzene, toluene, ethylbenzene and o-, m-, p-xylenes (BTEX) and polyromantic hydrocarbons (PAH). Organochlorined pesticides and PCBs were found in higher level for Fierza Reservoir stations. Their concentrations for Black and White Drin samples were 5-10 times lower.

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Determination of MTBE, TBA and BTEX in Soil by Headspace Gas Chromatography-Mass Spectrometry

Cited by 13 publications

References 19 publications

Diversity of Benzylsuccinate Synthase-Like ( bssA ) Genes in Hydrocarbon-Polluted Marine Sediments Suggests Substrate-Dependent Clustering

Diversity of Benzylsuccinate Synthase-Like ( bssA ) Genes in Hydrocarbon-Polluted Marine Sediments Suggests Substrate-Dependent Clustering

Determination of some organic pollutants in waters rivers of Tirana city

Analysis of organic pollutants in water samples of White Drin, Black Drin and Fierza Reservoir

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