Determination of multiple antibiotics in agricultural soil using a quick, easy, cheap, effective, rugged, and safe method coupled with ultra‐high performance liquid chromatography‐tandem mass spectrometry

Abstract

In this study, we combined ultra-high performance liquid chromatography with tandem mass spectrometry to establish a quick, easy, cheap, effective, rugged, and safe method of detecting 21 target antibiotics in agricultural soil samples.Antibiotics were extracted with mixed solvents consisting of ethylenediaminetetraacetic acid disodium salt dihydrate and phosphoric acid citric acid buffer and acetonitrile which were purified with octadecylsilyl as an adsorbent and anhydrous sodium sulfate as a desiccant. This method was able to effectively extract all of the target antibiotics from agricultural soils, with recovery efficiencies ranging from 55 to 108% and limits of detection between 0.09-0.68 μg/kg. We also validated this new method for selectivity, sensitivity, and reliability of detecting multiple antibiotics in 12 samples. Considering the potential environmental and public health effects of antibiotics in agricultural soils, our new method can help analyze the degree of antibiotic contamination and provide valuable information for soil quality and risk assessment.

Controlled synthesis of Cu-MOF possessing peroxidase-mimetic activity for the colorimetric detection of tetracycline in aqueous solution

“…Currently, several approaches have been developed for the detection of antibiotics, such as the enzyme-linked immunosorbent assay (ELISA), 8 Raman spectroscopy, 9 surface plasmon resonance (SPR) sensors, 10 fluorometric detection, 11 high-performance liquid chromatography (HPLC), 12 capillary electrophoresis, 13 colorimetric sensors, 14 and electrochemical detection. 15 Nonetheless, while most of these conventional techniques offer high sensitivity and accuracy, they can only be performed in well-equipped laboratories by trained personnel.…”

Ethanol‐ammonium sulfate system based modified quick, easy, cheap, effective, rugged, and safe method for the determination of four neonicotinoid pesticide and metabolite residues in two canned fruits

“…Thus, an effective clean-up step is needed to remove these high solubility impurities [18]. A series of cleanup sorbents including graphitized carbon black, N-octadecylsilane (C18), primary secondary amine, and carbon nanotubes (CNTs) have been investigated in QuEChERS methods for the removal of various impurities such as sugar, fat, lipids and pigments [19][20][21]. Multiwall CNTs (MWCNTs) and their derivatives with numerous hydroxyl groups, unique structure, large surface area, and ease of modification are especially attractive sorbents in the modified QuEChERS methods for the detection of pesticides in complex processed foods such as canned fruit [22].…”

Quantification of 16 cephalosporins in the aquatic environment by liquid chromatography‐tandem mass spectrometry

“…Furthermore, it has been found that antibiotics can cause mitochondrial proteotoxic stress and alter chloroplast function, gene expression, and cell proliferation [8]. Antibiotic concentrations in agricultural soils may also disturb germination, diminish biomass, and consequently lower production in farms fertilized with contaminated manure [9,10]. Furthermore, antibiotic residues can affect the human microbiota and produce health problems such as allergic reactions, persistent toxic effects after long-term exposure, and digestive system disturbance [10,11].…”