Determination of mutagenic sensitivity of hardwood cuttings of grapes ‘Red Globe’ and ‘Muscat’ ( Vitis vinifera L.) to gamma rays

Surakshitha, N.C.; Soorianathasundaram, K.; Ganesan, N. Meenakshi

doi:10.1016/j.scienta.2017.08.040

Cited by 21 publications

(14 citation statements)

References 5 publications

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“…Regarding to the effect of gamma ray doses, there were no significant differences among all doses of gamma rays in both seasons. The results obtained in the present investigation are similar to those of (Saiful et al,2015, Tayyer et al,2003, Dardeniz et al,2005, Surakshitha et al,2017.…”

Section: B Shoot Length (Cm)supporting

confidence: 92%

Genetic Diversity and Relationships Among Grapevine Rootstock Mutants Through Rapd Technique

Salama

Zayan

Shereif

et al. 2020

Journal of Productivity and Development

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Mutation using radiation has been shown to be an important tool in incorporating specific desirable agronomic value characteristics. In the present study, four grapevine rootstocks namely: Freedom, Harmony, SO4 and Ramsey along with commercial variety Thompson seedless were exposed to different doses of gamma rays (0, 10, 20 Gy) to obtain mutations with economic values.Results showed that both doses of 10 and 20 Gy were effective in obtaining mutations in all grapevine genotypes including Thompson seedless, which were confirmed by morphological markers such as bud burst percentage and shoot length and number of leaves, and genetic markers by RAPD technique.In addition, genetic variation and relationships in both irradiated and un-irradiated genotypes were determined. Six RAPD markers were able to generate polymorphic bands ranged from 1 to 9 among irradiated and un-irradiated grapevine genotypes. The similarity coefficients detected by RAPD markers ranged from 0.24 to 0.87, which revealed clear genetic variation among irradiated and un-irradiated grapevine genotypes. According to phylogenetic analysis, genotypes were divided into two clusters, where all five un-irradiated genotypes and irradiated Freedom genotype with 10 Gy were placed in one cluster and second cluster included the rest of nine irradiated genotypes.Conclusively, this study supports the use of mutation in breeding grapevine improvement, and RAPD technique could be an important tool for detecting mutations and genetic diversity among grapevine genotypes.

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Section: B Shoot Length (Cm)supporting

confidence: 92%

Genetic Diversity and Relationships Among Grapevine Rootstock Mutants Through Rapd Technique

Salama

Zayan

Shereif

et al. 2020

Journal of Productivity and Development

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“…Median lethal dose (LD 50 ) is an important indicator for analysing the sensitivity of plants to irradiation, which has been used as an important reference value to select the optimal irradiation dose in mutation breeding ( Surakshitha, Soorianathasundaram & Ganesan, 2017 ). Our study showed that the LD 50 was 54.28-Gy for the red freesia and 60.11-Gy for the purple freesia, suggesting that the red freesia is more sensitive to EBTTX irradiation than the purple freesia.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, under different EBTTX irradiation doses, the survival rates of the two freesia varieties slightly increased as the growth time increased from 30 to 80 days, suggesting that freesia alleviates the irradiation toxicity to themselves with increasing growth time by developing antioxidant defence systems for scavenging the overproduction of free radicals induced by irradiation (Wang et al, 2018). Median lethal dose (LD 50 ) is an important indicator for analysing the sensitivity of plants to irradiation, which has been used as an important reference value to select the optimal irradiation dose in mutation breeding (Surakshitha, Soorianathasundaram & Ganesan, 2017). Our study showed that the LD 50 was 54.28-Gy for the red freesia and 60.11-Gy for the purple freesia, suggesting that the red freesia is more sensitive to EBTTX irradiation than the purple freesia.…”

Section: Plant Growthmentioning

confidence: 99%

Biological effects of electron beam to target turning X-ray (EBTTX) on two freesia (Freesia hybrida) cultivars

Liu

Wang

et al. 2021

PeerJ

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Electron beam to target turning X-ray (EBTTX) is an emerging irradiation technology that can potentially accelerate the breeding process of plants. The biological effects of EBTTX irradiation on the two freesia cultivars (the red freesia and the purple freesia) were investigated by establishing an irradiation-mediated mutation breeding protocol. The germination rate, survival rate, plant height, leaf number and area, root number and length of the two freesia cultivars decreased following different irradiation doses (25, 50, 75, and 100-Gy). A high irradiation dose exhibited stronger inhibition effects on these plant growth parameters, and the survival rate of the two freesia cultivars was 0.00% following the 100-Gy irradiation treatment. The median lethal dose (LD50) based on survival rates was 54.28-Gy for the red freesia and 60.11-Gy for the purple freesia. The flowering rate, flower number, and pollen vigor were significantly decreased by irradiation treatment. At 75-Gy irradiation, the flowering rate, flower number and pollen viability of the two varieties reached the minimum, exhibiting strong inhibitory effects. Meanwhile, 75-Gy irradiation significantly decreased the chlorophyll content and increased the malondialdehyde (MDA) content of the two freesia cultivars. Furthermore, as the irradiation dose increased, the changes in the micro-morphology of the leaf epidermis and pollen gradually increased according to a scanning electron microscope (SEM) analysis. These results are expected to provide useful information for the mutation breeding of different freesia cultivars and other flowering plants.

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“…Low radiation can cause a smaller or larger vitality improvement. Meanwhile, the high radiation doses induce radio inhibition by affecting growth regulators and secondary metabolites and, eventually, tissue destruction in most cases (Surakshitha and Soorianathasundaram, 2017;Mostafa et al, 2019). Many authors so far reported a reduction of regenerative capacity and malformation of plant tissues, as well as, tissue destruction due to the high dose of radiation (Chakravarty and Sen, 2001;Yamaguchi, 2018;Andrew-Peter-Leon et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Radio sensitivity tests are essential for determining the appropriate radiation dose to induce the highest genetic diversity with the most useful fixed mutations (Songsri et al, 2019;Riviello-Flores et al, 2022). Studies also stated that irradiation with low radiation doses stimulates the plants' vitality, which is beneficial for plant regeneration (Jala and Bodhipadma, 2011;Abubakar et al, 2017;Surakshitha and Soorianathasundaram, 2017;Aisyah et al, 2021). Some studies have reported radiation stimulation by GR in some plant varieties of Ocimum basilicum or cowpea (Enkhbileg et al, 2019;Aisyah et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Molecular, Phenotypic Marker Assays, and Radiosensitivity Tests of Gamma-Irradiated Celosia Argentea

KURUCZ¹,

ANDRE²,

FARI³

et al. 2022

SABRAOJBG

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Random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), and UPOV phenotype markers were used to study the DNA polymorphism in gamma-ray induced morphological mutants of Celosia argentea var. plumosa Hungarian variety ‘Arrabona.‘ In the experiments, the study determined the radio sensitivity and the genetic diversity of gamma radiation of C. argentea var. plumosa ‘Arrabona.‘ Seeds of C. argentea var. plumosa ‘Arrabona‘ were irradiated with gamma rays to increase their genetic diversity. The irradiation doses consisted of 0, 75, 150, 300, 450, and 600 Grays (Gy). The germination percentage, survival rate, and phenotype of irradiated plantlets underwent evaluation in the first (M1) and second (M2) generations. The investigation of genetic diversity used the ISSR and RAPD primers. Based on the results, the first-generation genetic distance increased as the doses increased. But the trend changed considerably through the generation due to the low condition and fertility of the high doses of gamma-irradiated plants. These individuals did not show at the next mutant generation, changing the population gene pool. In addition, open pollination has also changed genetic diversity. The RAPD and ISSR primers proved proper to evaluate the genetic diversity, nonetheless fewer direct connection occurred between the appearance and the used RAPD or ISSR markers. The LD50 dose between 150 and 300 Gray treatments and the radiation between 300450 Gray induced the median growth reduction in the mutant ‘Arrabona‘ population. Based on these results, the study concluded that both UPOV-based phenotyping and molecular marker analysis revealed appropriate for determining genetic divergence, but detecting greater genetic distance resulted in molecular markers.

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Determination of mutagenic sensitivity of hardwood cuttings of grapes ‘Red Globe’ and ‘Muscat’ ( Vitis vinifera L.) to gamma rays

Cited by 21 publications

References 5 publications

Genetic Diversity and Relationships Among Grapevine Rootstock Mutants Through Rapd Technique

Genetic Diversity and Relationships Among Grapevine Rootstock Mutants Through Rapd Technique

Biological effects of electron beam to target turning X-ray (EBTTX) on two freesia (Freesia hybrida) cultivars

Molecular, Phenotypic Marker Assays, and Radiosensitivity Tests of Gamma-Irradiated Celosia Argentea

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