Determination of Mycotoxin Production of Fusarium Species in Genetically Modified Maize Varieties by Quantitative Flow Immunocytometry

Abstract

Levels of mycotoxins produced by Fusarium species in genetically modified (GM) and near-isogenic maize, were determined using multi-analyte, microbead-based flow immunocytometry with fluorescence detection, for the parallel quantitative determination of fumonisin B1, deoxynivalenol, zearalenone, T-2, ochratoxin A, and aflatoxin B1. Maize varieties included the genetic events MON 810 and DAS-59122-7, and their isogenic counterparts. Cobs were artificially infested by F. verticillioides and F. proliferatum conidia, and contained F. graminearum and F. sporotrichoides natural infestation. The production of fumonisin B1 and deoxynivalenol was substantially affected in GM maize lines: F. verticillioides, with the addition of F. graminearum and F. sporotrichoides, produced significantly lower levels of fumonisin B1 (~300 mg·kg−1) in DAS-59122-7 than in its isogenic line (~580 mg·kg−1), while F. proliferatum, in addition to F. graminearum and F. sporotrichoides, produced significantly higher levels of deoxynivalenol (~18 mg·kg−1) in MON 810 than in its isogenic line (~5 mg·kg−1). Fusarium verticillioides, with F. graminearum and F. sporotrichoides, produced lower amounts of deoxynivalenol and zearalenone than F. proliferatum, with F. graminearum and F. sporotrichoides. T-2 toxin production remained unchanged when considering the maize variety. The results demonstrate the utility of the Fungi-Plex™ quantitative flow immunocytometry method, applied for the high throughput parallel determination of the target mycotoxins.

Detection of Aflatoxins in Different Matrices and Food-Chain Positions

“…Flow cytometry based competitive fluorescent microsphere immunoassay (CFIA) is a microbead-based competitive fluorescent immunoassay applying monoclonal antibodies of high affinity. It can simultaneously detect six mycotoxins (OTA, AFB1, FB1, DON, T2, ZEA) with increased sensitivity for aflatoxins (0.12 μg/kg) following a simple extraction procedure compared to an ELISA method ( Czéh et al, 2012 ; Czéh, 2014 ; Bánáti et al, 2017 ).…”

Aspergillus-Derived Mycotoxins In The Feed And Food Chain

“…The recovery was between 87 and 113%. AFB1, FB1, DON, T2, ZEA) with increased sensitivity for aflatoxins (0.12 µg/kg) following a simple extraction procedure compared to an ELISA method (Czéh et al, 2012;Czéh, 2014;Bánáti et al, 2017).…”