Determination of mycotoxins in bovine milk by liquid chromatography tandem mass spectrometry

Sørensen, Lambert K.; Elbaek, Tina H

doi:10.1016/j.jchromb.2005.03.020

Cited by 187 publications

(118 citation statements)

References 53 publications

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“…To evaluate the OTA in milk samples considered in the present paper, the method described by Sørensen and Elboek (2005) (defattening with hexane and extraction with acetonitrile) has been initially applied. In a second time, to verify the absence of compounds potentially interfering with the retention time of OTA, a number of representative blank milk samples of different origin were analysed.…”

Section: Resultsmentioning

confidence: 99%

“…Traces of OTA have been also found in human milk (Mantle, 2002;Turconi et al, 2004;Mantle, 2002, Turconi et al, 2004. With regard to ruminants' milk, several studies have demonstrated that OTA can be hydrolysed by rumen microflora and rumen pH to a less toxic metabolite, the ochratoxin α (Boudra & Morgavi, 2006;Sørensen & Elboek, 2005;Boudra and Morgavi, 2006;Sørensen and Elboek, 2005). So bovine milk should not be considered as an important source of OTA.…”

Section: Introductionmentioning

confidence: 99%

“…More recently, other authors have considered the same matter (Boudra & Morgavi, 2006;Sørensen & Elboek, 2005;Boudra and Morgavi, 2006;Sørensen and Elboek, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Detection and quantification of Ochratoxin A in milk produced in organic farms

2011

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection and quantification of Ochratoxin A in milk produced in organic farms

2011

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“…Chloramphenicol (CAP), a highly active, broad-spectrum antibiotic produced by Streptomyces venezuelae ( Van de Water and Haagsma 1987;Jeya Shakila et al 2007), has satisfactory inhibition on Gram-negative and Gram-positive bacteria (Sorensen et al 2003). Since CAP was first isolated in 1947 (Volini et al 1950), it had been used clinically for the treatment of bacteriosis and it had been used as a feed additive in animal breed and aquaculture because of its, excellent antibacterial and stable medicinal properties and low price (Holt et al 1993;Turton et al 1999;Farombi 2001).…”

Section: Introductionmentioning

confidence: 99%

Preparation and purification of monoclonal antibodies against chloramphenicol

Wang

et al. 2011

Cytotechnology

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Monoclonal antibodies (McAbs) against chloramphenicol (CAP) were produced to detect CAP residues, which could be toxic and possesses a potential threat to human health. The CAP-BSA conjugate was obtained by bovine serum albumin (BSA) coupled with CAP, and used to immunize the mice. The splenocytes from the immunized mice were fused with mouse myeloma cells SP2/0 to form hybridoma, which may secrete McAbs against CAP. Hybridomas 1D 1 and 3G 12 secreting McAbs against CAP were obtained by screening. Ascites containing McAbs were prepared by injecting 1 x 10 6 cells of hybridoma 1D 1 and 3G 12 into the abdomen of mice. Protein A affinity chromatography was used to purify McAbs against CAP in a single chromatographic step with recovery yield above 80% and purity above 95% and full recovery of antibody activity. Experiments showed that McAb 3G 12 was highly specific for CAP and had no cross-reactivity with analogues which have a structure similar to CAP. The IC 50 value was 50.8 ng/mL.

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“…One part of antibiotics was deposited to the pond bottom and the others remained in pond water. Chloramphenicol (CAP) is a broad spectrum antibiotic exhibiting activity against both gram-positive and gram-negative bacteria as well as other groups of micro-organisms (Sorensen et al, 2003;Park and Kim, 2006;Shakila et al, 2006). CAP is commonly used in aquaculture as a prophylactic or disinfectant to prevent diseases, or as a chemotherapeutic agent to control diseases.…”

Section: Introductionmentioning

confidence: 99%

Preliminary investigation of chloramphenicol in fish, water and sediment from freshwater aquaculture pond

Dang

Yang

2009

Int. J. Environ. Sci. Technol.

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Analytical methods of chloramphenicol in the aquaculture environment have been developed using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry. The contents of chloramphenicol were determined using high-performance liquid chromatography for sediment and liquid chromatography-tandem mass spectrometry for fish and water collected from a freshwater aquaculture pond in China. Chloramphenicol in the water and sediment were 112.3 ng/L and 0.1957 mg/kg, respectively. The chloramphenicol residues in 3 kinds of fish, including carp, chub and grass carp were different. Only the muscle and head of grass carp were under the minimum required performance limit (0.3 µg/kg) and were safe to eat. The chloramphenicol in other tissues of grass carp, carp and chub exceeded the minimum required performance limit. The highest content of chloramphenicol was in the branchia of carp and the lowest was in the head of grass carp. The results showed the chloramphenicol in the aquaculture environment was serious, although the government of China had banned the use of chloramphenicol in aquaculture a few years ago.

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Determination of mycotoxins in bovine milk by liquid chromatography tandem mass spectrometry

Cited by 187 publications

References 53 publications

Detection and quantification of Ochratoxin A in milk produced in organic farms

Detection and quantification of Ochratoxin A in milk produced in organic farms

Preparation and purification of monoclonal antibodies against chloramphenicol

Preliminary investigation of chloramphenicol in fish, water and sediment from freshwater aquaculture pond

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