Determination of Myriocin in Natural and Cultured Cordyceps cicadae Using 9-Fluorenylmethyl Chloroformate Derivatization and High-Performance Liquid Chromatography with UV-Detection

Yu, Jiawen; Xu, Huibi; Mo, Zhihong; Hua-li, Zhu; Mao, Xian-Bing

doi:10.2116/analsci.25.855

Cited by 22 publications

(20 citation statements)

References 22 publications

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“…Yu et al (2009) have reported that myriocin was detected in the wild sample from Chongqing (Latin name they used Cordyceps cicadae , but Chinese name they called vegetable cicada) and in its cultures, but not in the wild samples from Guizhou and Sichuan. In view of the fact that the content of myriocin in dozens of vegetable cicada from different laboratories (including Yu’s laboratory) and their cultures is less than the detection limit, so it may not be simply attributed to the differences among strains.…”

Section: Discussionmentioning

confidence: 99%

“…The residue was dissolved in 2 ml 50% methanol. After centrifugation at 12 000 r/min for 10 min with a low-temperature high-speed centrifuge (Thermo-Fisher, USA), the supernatant was transferred onto the ODS-solid-phase extraction (SPE) column (500 mg/3 ml, 40–60 μm, HongPu Scientific, Ningbo, China), which was pretreated with 10 ml methanol and 5 ml water (Yu et al 2009). The column was washed with 10% methanol (1 × 3 mL) and 50% methanol (2 × 3 mL) successively.…”

Section: Methodsmentioning

confidence: 99%

“…Many studies on the composition of vegetable cicada have been reported, but it is still controversy on whether it contains myriocin or not (Wang et al 2009; Yu et al 2009). Myriocin, a fungal metabolite, is a potent inhibitor of serine palmitoyltransferase (SPT), which is the key rate limiting enzyme in sphingolipid biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

“…However, long-term use of myriocin may produce adverse effects on health by modulating biological functions of cells as a result of persistent disruption of sphingolipid metabolism (He et al 2004). Several analytical methods to detect myriocin, such as HPLC-light scattering detection (Wang et al 2009), HPLC-fluorescence detection with a complex precolumn derivatisation by fluorenylmethyl-chloroformate (Yu et al 2009), HPLC-OPA-UV (Yu et al 2012) and HPLC-MS (Campisi et al, 2017), have been published. With the rapid development of high-performance liquid chromatography-mass spectrometry, a hybrid linear ion trap-quadrupole-Orbitrap high-resolution mass spectrometry (LTQ-Orbitrap-HRMS) combined with Fourier-transform technology, offers ultra-high resolving power with resolution up to 100,000.…”

Section: Introductionmentioning

confidence: 99%

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Identification and determination of myriocin in Isaria cicadae and its allies by LTQ-Orbitrap-HRMS

Cheng

Zhang

et al. 2017

Mycology

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A hybrid linear ion trap-quadrupole-Orbitrap high-resolution mass spectrometry (LTQ-Orbitrap-HRMS) was used to qualitatively and quantitatively analyse the myriocin in Isaria cicadae and its allies. The samples were prepared with 95% methanol for 30 min by ultrasonic-assisted extraction. The target compound was purified by ODS solid-phase extraction (SPE) column. The enriched samples were identified by mass spectrometry. The results showed that the contents of myriocin in both wild and artificial Isaria cicadae were below the detection limit, while a strain of Ophiocordyceps longissima and Cordyceps cicadae Shing (Dujiaolong), both closely related to the Isaria cicadae, and its asexual mycelia are rich in myriocin. It suggests that it may be wrong to consider C. cicadae as I. cicadae’s teleomorph in Genbank or Mycobank in many published reports based on chemical classification, and the species rich in myriocin is probably not Isaria cicadae.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and determination of myriocin in Isaria cicadae and its allies by LTQ-Orbitrap-HRMS

Cheng

Zhang

et al. 2017

Mycology

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“…In addition, a sphingosine-like immunosuppressant ISP-1 (myriocin) has been isolated from the natural fruit body and fermented mycelia of C. cicadae (Yu et al 2009). ISP-1 exhibited an immunosuppressive potency 10- to 100-fold greater than that of cyclosporin A, a widely used immunosuppressant, when the activity was measured in a mouse allogenic mixed lymphocyte reaction (Fujita et al 1994).…”

Section: Industry Of Fungi Associated With Chinese Cordycepsmentioning

confidence: 99%

Cordyceps industry in China

Dong

Guo²,

Wang

et al. 2015

Mycology

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Cordyceps, as a general term, describes a group of ascomycetous fungi growing on arthropods and other related fungi. Some cordyceps have been used in traditional Chinese medicine for centuries and cordyceps-derived products are currently a big industry in China. A number of medicinal and health products have been developed and extensively commercialized from natural Chinese cordyceps, its anamorphic fungus (Hirsutella sinensis), and other fungi known as Chinese cordyceps. The lack of a defined classification system for medicinal cordyceps fungi is a source of confusion in the industry and the public, and even among pharmaceutical scientists. This review summarizes the cordyceps fungi currently used in the industry in China with a special reference to clarify Chinese cordyceps and associated fungi. Cordyceps militaris, Cordyceps guangdongensis and Isaria cicadae are well recognized and commercialized cordyceps fungi in China. Except the natural Chinese cordyceps and its anamorphic fungus, Paecilomyces hepiali, Mortierella hepiali, Cephalosporium sinensis and Clonostachys rosea isolated from natural Chinese cordyceps are classified as Chinese cordyceps–associated fungi. P. hepiali is a cordyceps fungus based on current phylogenetic analysis of Hypocreales, while M. hepiali is a fungus in the Zygomycetes and should only be treated as associated fungus of Chinese cordyceps. C. sinensis and C. rosea belong to the Hypocreales and their relationship to cordyceps fungi should be further studied. The exploitation of the resources of cordyceps fungi and their quality control in the industry should be major topics for future studies. Cooperation between the industry and the research community will enhance the whole cordyceps industry.

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Determination of the serine palmitoyl transferase inhibitor myriocin by electrospray and Q‐trap mass spectrometry

Campisi

Signorelli

Rizzo

et al. 2017

Biomedical Chromatography

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Determination of Myriocin in Natural and Cultured Cordyceps cicadae Using 9-Fluorenylmethyl Chloroformate Derivatization and High-Performance Liquid Chromatography with UV-Detection

Cited by 22 publications

References 22 publications

Identification and determination of myriocin in Isaria cicadae and its allies by LTQ-Orbitrap-HRMS

Identification and determination of myriocin in Isaria cicadae and its allies by LTQ-Orbitrap-HRMS

Cordyceps industry in China

Determination of the serine palmitoyl transferase inhibitor myriocin by electrospray and Q‐trap mass spectrometry

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